Core E Research Support

摘要 由于数十年的重工业活动，北伯明翰地区受到了严重的 有毒化学物质，包括重金属，如镉、砷和锰。世界上的人口 建议的北伯明翰国家优先事项清单(NPL)地点(受影响地区；邮政编码35207和35217)是 主要是非洲裔美国人，慢性阻塞性肺疾病(COPD)的发病率较高，较低 呼吸道感染(LRTI)和哮喘与杰斐逊工业化程度较低地区(控制区)的比较 县(邮政编码35214)。考虑到美国肺部疾病的高患病率，特别是 对于慢性阻塞性肺病、哮喘和呼吸道感染，拟议的研究与公共卫生相关。《临床》 生物库和动物暴露核心(核心E)有两个具体目标：具体目标1：管理 鉴定、招募、知情同意程序、登记、临床数据收集和生物标本 收集生活在疫区和对照地区的受试者；具体目标2：暴露成年小鼠(C57BL/6) 男女接触砷、锰、镉或来自疫区和控制区的实际过滤粉尘4-6 每天工作三个小时；MICE将被转移到不同的项目，这样他们就可以完成他们提议的 明确的目标。临床生物资料库和动物暴露核心的活动将对总体 这项超级基金计划的成功。人体标本的综合生物储存库将构成一个 将推动NIEHS/超级基金使命的独特资源。此外，使小鼠暴露在重金属中 在环境空气中存在的浓度将创建一个模型，反映和概括 核电厂址的环境暴露。核心将由一名具有丰富经验的肺科医生组成 在临床研究中，一位在老鼠吸入和肺损伤研究方面拥有丰富经验的毒理学家 协调员和一名研究助理。核心活动将紧密结合在一起并得到数据的支持 管理和分析核心(DMAC)、社区参与核心(CEC)、生物信息库基础设施 UAB临床翻译科学中心(CCTS)和UAB内的吸入暴露设施 动物设施。所有研究人员都将被要求满足并保持UAB的培训要求 IRB和IACUC。IRB和IACUC将审查并监督所有与研究有关的活动。 程序将到位，以确保人类受试者的安全和对脊椎动物的人道处理。这个 UAB CCTS生物库遵循国际协会定义的最佳实践，以确保验证和 为样品处理和样品完整性制定了可靠的程序和协议。集中式、 此核心的标准化和全面的专业知识和组织代表着基于价值的 为整个拟建中心所做的努力。

ABSTRACT Due to decades of heavy industrial activity, the North Birmingham area has been severely polluted with toxic chemicals including heavy metals such as cadmium, arsenic, and manganese. The population in the proposed North Birmingham National Priorities List (NPL) site (Affected Areas; zip codes 35207 and 35217) is predominantly African American and has higher rates of chronic obstructive pulmonary disease (COPD), lower respiratory tract infection (LRTI), and asthma compared to less industrialized areas (Control Area) of Jefferson County (zip code 35214). Considering the high prevalence of lung disease in the United States, particularly COPD, asthma, and respiratory tract infections, the proposed research is relevant to public health. The Clinical Biorepository and Animal Exposure Core (Core E) has two specific aims: Specific Aim 1: Manage the identification, recruitment, informed consent process, enrollment, clinical data collection, and biological specimen collection from subjects living in the Affected and Control Areas; Specific Aim 2: Expose adult mice (C57BL/6) of both sexes to arsenic, manganese, cadmium or real-life filtered dust from Affected and Control Areas for 4-6 hrs per day for three weeks; mice will be transferred to various projects so they can complete their proposed specific aims. The activities of the Clinical Biorepository and Animal Exposure Core will be essential to the overall success of this Superfund Program. The comprehensive biorepository of human specimens would constitute a unique resource that would further the NIEHS/Superfund mission. Furthermore, exposing mice to heavy metals in concentrations that are present in ambient air will create a model that mirrors and recapitulates the environmental exposures at the NPL site. The Core will be comprised of a pulmonologist with ample experience in clinical research, a toxicologist with extensive experience in mouse inhalation and lung injury study, a research coordinator, and a research associate. Core activities will be closely integrated and supported by the Data Management and Analysis Core (DMAC), Community Engagement Core (CEC), the biorepository infrastructure of the UAB Center for Clinical Translational Science (CCTS), and the Inhalation Exposure Facility within the UAB Animal Facility. All research personnel will be required to fulfill and maintain the training requirements of the UAB IRB and the IACUC. The IRB and the IACUC will review and provide oversight of all research-related activities. Procedures will be in place to ensure safety of human subjects and humane handling of vertebrate animals. The UAB CCTS Biorepository follows best practices defined by international societies to ensure that validated and reliable procedures and protocols are in place for specimen handling and sample integrity. The centralized, standardized and comprehensive expertise and organization of this Core represents a value-based, economy of effort for the overall proposed center.