Core E Research Support

摘要 由于数十年的重工业活动，北伯明翰地区受到严重污染， 有毒化学物质，包括镉、砷和锰等重金属。的人口 拟议的北伯明翰国家优先事项清单（NPL）网站（受影响地区;邮政编码35207和35217）是 主要是非洲裔美国人，慢性阻塞性肺疾病（COPD）的发病率较高， 呼吸道感染（LRTI）和哮喘与杰斐逊工业化程度较低的地区（对照区）相比， 县（邮编35214）。考虑到美国肺部疾病的高患病率，特别是 COPD、哮喘和呼吸道感染，拟议的研究与公共卫生有关。临床 生物储存库和动物暴露核心（核心E）有两个具体目标：具体目标1：管理 识别、招募、知情同意过程、入组、临床数据收集和生物标本 从生活在受影响地区和对照地区的受试者中采集;具体目标2：暴露成年小鼠（C57 BL/6） 受影响地区和控制地区的砷、锰、镉或实际过滤粉尘的影响4-6 三周内，小鼠将被转移到不同的项目中，以便它们能够完成他们的计划。 具体目标。临床生物储存库和动物暴露核心的活动对于整个 这个超级基金项目的成功。人类标本的综合生物库将构成一个 独特的资源，将进一步NIEHS/超级基金使命。此外，将老鼠暴露在重金属中 将创建一个模型，反映和概括 NPL现场的环境暴露。核心将由经验丰富的肺科医生组成 在临床研究方面，一位在小鼠吸入和肺损伤研究方面具有丰富经验毒理学家， 协调员和研究助理。核心活动将与数据紧密结合，并得到数据的支持。 管理和分析核心（DMAC），社区参与核心（CEC），生物储存库基础设施 UAB临床转化科学中心（CCTS）和UAB内的吸入暴露机构 动物设施。所有研究人员都必须满足并保持UAB的培训要求 IRB和IACUC。IRB和IACUC将审查并监督所有研究相关活动。 将制定程序，以确保人类受试者的安全性和脊椎动物的人道处理。的 UAB CCTS生物储存库遵循国际协会定义的最佳实践，以确保经过验证和 对于样本处理和样本完整性，有可靠的程序和协议。中央集权， 标准化和全面的专业知识和组织，这一核心代表了一个基于价值的经济， 为整个中心的建设而努力。

ABSTRACT Due to decades of heavy industrial activity, the North Birmingham area has been severely polluted with toxic chemicals including heavy metals such as cadmium, arsenic, and manganese. The population in the proposed North Birmingham National Priorities List (NPL) site (Affected Areas; zip codes 35207 and 35217) is predominantly African American and has higher rates of chronic obstructive pulmonary disease (COPD), lower respiratory tract infection (LRTI), and asthma compared to less industrialized areas (Control Area) of Jefferson County (zip code 35214). Considering the high prevalence of lung disease in the United States, particularly COPD, asthma, and respiratory tract infections, the proposed research is relevant to public health. The Clinical Biorepository and Animal Exposure Core (Core E) has two specific aims: Specific Aim 1: Manage the identification, recruitment, informed consent process, enrollment, clinical data collection, and biological specimen collection from subjects living in the Affected and Control Areas; Specific Aim 2: Expose adult mice (C57BL/6) of both sexes to arsenic, manganese, cadmium or real-life filtered dust from Affected and Control Areas for 4-6 hrs per day for three weeks; mice will be transferred to various projects so they can complete their proposed specific aims. The activities of the Clinical Biorepository and Animal Exposure Core will be essential to the overall success of this Superfund Program. The comprehensive biorepository of human specimens would constitute a unique resource that would further the NIEHS/Superfund mission. Furthermore, exposing mice to heavy metals in concentrations that are present in ambient air will create a model that mirrors and recapitulates the environmental exposures at the NPL site. The Core will be comprised of a pulmonologist with ample experience in clinical research, a toxicologist with extensive experience in mouse inhalation and lung injury study, a research coordinator, and a research associate. Core activities will be closely integrated and supported by the Data Management and Analysis Core (DMAC), Community Engagement Core (CEC), the biorepository infrastructure of the UAB Center for Clinical Translational Science (CCTS), and the Inhalation Exposure Facility within the UAB Animal Facility. All research personnel will be required to fulfill and maintain the training requirements of the UAB IRB and the IACUC. The IRB and the IACUC will review and provide oversight of all research-related activities. Procedures will be in place to ensure safety of human subjects and humane handling of vertebrate animals. The UAB CCTS Biorepository follows best practices defined by international societies to ensure that validated and reliable procedures and protocols are in place for specimen handling and sample integrity. The centralized, standardized and comprehensive expertise and organization of this Core represents a value-based, economy of effort for the overall proposed center.