Comparative Pathology Shared Resource

比较病理学共享资源

• 批准号: 10576828
• 负责人: M. Gerard O'Sullivan
• 金额: $ 10.31万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10576828
• 关键词: Animal Model; Animals; Applications Grants; Autopsy; Blood; Board Certification; Cancer Center; Cancer Center Support Grant; Clinical Chemistry; Comparative Pathology; Consult; Data; Doctor of Philosophy; Ensure; Evaluation; Feedback; Formalin; Freezing; Funding; Grant; Hematology; Histologic; Histology; Histopathology; Image Analysis; Immunohistochemistry; Label; Laboratories; Laboratory Animals; Malignant Neoplasms; Manuscripts; Measurement; Microscopic; Mission; Pathologic; Pathologist; Pathology; Phenotype; Postdoctoral Fellow; Preparation; Process; Proteins; Protocols documentation; Publications; Research; Research Design; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Specimen; Techniques; Testing; Tissues; Transgenic Animals; Veterinary Pathology; animal tissue; bone; comparative; digital imaging; experience; impression; interest; meetings; member; morphometry; operation; tissue fixing

Comparative Pathology Shared Resource Summary The mission of the Comparative Pathology Shared Resource (CPSR) is to provide comprehensive pathology support for Masonic Cancer Center (MCC) members who use laboratory animals in their research. The CPSR is a critical resource for projects using animal tissues and is essential for publications involving pathological changes in tissues. The CPSR operation has been led by M. Gerard O'Sullivan, MVB, PhD, since 2009 and is supported by Technical Coordinator Katalin Kovacs, PhD; histotechnologist Paula Overn, HT, QIHC; and postdoctoral research pathologist, Flaviu Tabaran DVM, PhD. Dr. O'Sullivan has more than 25 years of experience in comparative and experimental veterinary pathology. The laboratory prepares appropriately processed fixed or frozen tissues for histology and immunohistochemistry (IHC). Particular strengths of the laboratory are in doing non-routine, challenging, or tedious histological preparations; performing IHC preparations on multiple markers; developing new IHC protocols for markers of interest; and performing IHC on bone. Board-certified veterinary pathologists provide expert gross and histopathologic analysis, generate high- resolution digital images of specimens, and provide intellectual input on study design and manuscript preparation for research projects and grant applications. The CPSR also has considerable experience in developing new protocols and techniques and has developed 121 new IHC markers since 2012. In addition, the CSPR has developed double-labeling techniques to simultaneously detect several combinations of proteins. Requests for quantification of marker expression and histomorphometric evaluations have increased, and CSPR has developed expertise to accommodate these requests. Weekly staff meetings and annual feedback from CPSR users ensure that the resource is able to meet the needs of MCC members.

比较病理学共享资源摘要 比较病理学共享资源(CPSR)的使命是提供全面的病理学 为共济会癌症中心(MCC)的成员提供支持，他们在研究中使用实验室动物。CPSR 是使用动物组织的项目的关键资源，对于涉及病理学的出版物也是必不可少的 组织的变化。CPSR业务自2009年以来一直由M.Gerard O‘Sullivan，MVB，PhD领导，目前 由技术协调员Katalin Kovacs博士、组织技术学家Paula Overn、HT、QIHC支持； Flaviu Tabaran DVM博士，博士后研究病理学家。奥沙利文博士有超过25年的 具有比较和实验兽医病理学方面的经验。实验室做了适当的准备 经处理的固定或冷冻组织，用于组织学和免疫组织化学(IHC)。具有独特优势的 实验室正在进行非常规的、具有挑战性的或繁琐的组织学准备；进行IHC 对多个标记进行准备；为感兴趣的标记开发新的IHC方案；以及对 骨头。委员会认证的兽医病理学家提供专业的大体和组织病理学分析，产生高 解析标本的数字图像，并为研究设计和手稿提供智力输入 准备研究项目和拨款申请。CPSR还在以下方面拥有丰富的经验 开发新的方案和技术，并自2012年以来开发了121个新的IHC标记物。此外, CSPR已经开发了双重标记技术来同时检测几种组合的 蛋白质。对标记物表达和组织形态计量学评估的量化要求增加， CSPR已经开发了专门知识来满足这些要求。每周员工会议和年度会议 CPSR用户的反馈确保资源能够满足MCC成员的需求。