Comparative Pathology Shared Resource

比较病理学共享资源摘要 比较病理学共享资源（CPSR）的使命是提供全面的病理学 支持共济会癌症中心（MCC）成员谁使用实验室动物在他们的研究。CPSR 是使用动物组织的项目的关键资源，对于涉及病理学的出版物是必不可少的。 组织的变化。CPSR行动由M. Gerard O 'Sullivan，MVB，博士，自2009年以来， 由技术协调员Katalin Kovacs博士、组织技术专家Paula Overn、HT、QIHC提供支持; 博士后研究病理学家，Flaviu Tabaran DVM，PhD。奥沙利文博士有超过25年的 具有比较和实验兽医病理学经验。实验室做好准备 经处理的固定或冷冻组织用于组织学和免疫组织化学（IHC）。特别优势的 实验室正在进行非常规的、具有挑战性的或繁琐的组织学制备;进行IHC 针对多种标记物的免疫组化制备;针对感兴趣的标记物开发新的免疫组化方案;以及 骨头委员会认证的兽医病理学家提供专家的大体和组织病理学分析，产生高- 标本的高分辨率数字图像，并为研究设计和手稿提供智力输入 准备研究项目和赠款申请。CPSR在以下方面也有相当丰富的经验： 自2012年以来，该公司开发了121种新的IHC标记物。此外，本发明还提供了一种方法， CSPR已经开发了双标记技术，以同时检测以下几种组合： proteins.对标记物表达和组织形态学评价的定量要求有所增加， CSPR已经开发了专门知识来满足这些要求。每周工作人员会议和 CPSR用户的反馈确保资源能够满足MCC成员的需求。

Comparative Pathology Shared Resource Summary The mission of the Comparative Pathology Shared Resource (CPSR) is to provide comprehensive pathology support for Masonic Cancer Center (MCC) members who use laboratory animals in their research. The CPSR is a critical resource for projects using animal tissues and is essential for publications involving pathological changes in tissues. The CPSR operation has been led by M. Gerard O'Sullivan, MVB, PhD, since 2009 and is supported by Technical Coordinator Katalin Kovacs, PhD; histotechnologist Paula Overn, HT, QIHC; and postdoctoral research pathologist, Flaviu Tabaran DVM, PhD. Dr. O'Sullivan has more than 25 years of experience in comparative and experimental veterinary pathology. The laboratory prepares appropriately processed fixed or frozen tissues for histology and immunohistochemistry (IHC). Particular strengths of the laboratory are in doing non-routine, challenging, or tedious histological preparations; performing IHC preparations on multiple markers; developing new IHC protocols for markers of interest; and performing IHC on bone. Board-certified veterinary pathologists provide expert gross and histopathologic analysis, generate high- resolution digital images of specimens, and provide intellectual input on study design and manuscript preparation for research projects and grant applications. The CPSR also has considerable experience in developing new protocols and techniques and has developed 121 new IHC markers since 2012. In addition, the CSPR has developed double-labeling techniques to simultaneously detect several combinations of proteins. Requests for quantification of marker expression and histomorphometric evaluations have increased, and CSPR has developed expertise to accommodate these requests. Weekly staff meetings and annual feedback from CPSR users ensure that the resource is able to meet the needs of MCC members.