Facile screening for esophageal cancer in LMICs
Facile screening for esophageal cancer in LMICs
批准号:
10238011
负责人:
Stephen J Meltzer
金额:
$93.63万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-05-01 至 2024-08-31
关键词:
Aberrant DNA MethylationBackBenignBiological AssayBiological MarkersCar PhoneCellsCellular PhoneCessation of lifeChIP-on-chipChargeClinicClinicalCold ChainsCommunity HealthCoupledCytologyDNADNA MethylationDataDefectDeglutitionDepositionDetectionDevicesDiagnosisDiagnosticDiagnostic TrialEarly DiagnosisElectromagneticsEndoscopyEsophageal Squamous Cell CarcinomaEsophageal TissueEsophagusEventFeedbackFeesFreeze DryingGoldGuidelinesHealth PersonnelHumanHypermethylationImageInterventionLesionLiquid substanceMalignant NeoplasmsMalignant neoplasm of esophagusMeasurementMediatingMedicalMethodsMethylationMicrofluidic MicrochipsMucous MembraneNanotechnologyPatientsPerformancePhasePolymethyl MethacrylatePopulationPoriferaPrognosisProtocols documentationQuality ControlReagentRehydrationsResourcesSamplingScreening for cancerSensitivity and SpecificityServicesSilicon DioxideSiteSpecimenSystemTalentsTelephoneTestingTimeTubeUgandaUniversitiesValidationadvanced diseasebasebisulfitecarcinogenesisclinical translationcostcost effectivecost effectivenessdetection platformdiagnostic accuracydiagnostic platformearly detection biomarkersepigenetic markeresophageal squamous cell cancerfollow-uphigh riskimprovedinstrumentisothermal amplificationlow and middle-income countriesmethylation testingmicrochipminimally invasivemortalitynoninvasive diagnosispoint of carepoint-of-care diagnosticsportabilityprinted circuit boardprototypesample collectionscreeningsuperparamagnetic beadsuser-friendly
中文摘要
点击翻译按钮获取中文摘要
英文摘要
Esophageal squamous cell carcinoma (ESCC) ranks sixth among all cancers worldwide, with 450,000 new
cases diagnosed per year and a very poor prognosis. Low-cost, minimally invasive point-of-care population
screening for ESCC is badly needed, particularly in LMICs, where 5-year ESCC survival is less than 10%.
Altered methylation occurs frequently in human malignancies, including EC, constituting an early event that
can serve as an early cancer detection biomarker. However, for DNA methylation to be used in this manner,
we need cost-effective, user-friendly and robust tests that permit clinical translation in LMICs. We propose an
early ESCC diagnostic strategy comprising a single-use, swallowable sponge to collect esophageal specimens
coupled with a smartphone-manipulated microfluidic chip for automated sample processing and methylation
detection. This strategy does not require endoscopy (EGD), can be administered by healthcare workers
without medical degrees, and uses an on-phone analytic app. The sponge is cheaper (approximately $30
each), less invasive, and easier to perform than EGD ($1500 total cost, including facility fees); there are no
room charges, unlike EGD. The microchip integrates DNA extraction, bisulfite DNA conversion, and
methylation analysis into a single device. In addition, the microchip interfaces with a cellphone, for both device
control and methylation detection and analysis. The integrated device enables detection of DNA methylation
from crude samples in a “sample-to-answer” manner, without the need for sending data back to an analytic
center off-site. Thus, the proposed platform promises a cost-effective and user-friendly POC strategy for early
ESCC detection that is implementable in LMICs. We have also assembled a talented interdisciplinary,
intercontinental team to execute this strategy. Our task will be achieved in 2 phases via the following Aims:
UG3 PHASE: Aim 1: To assess a streamlined protocol for sample collection, processing and methylation
detection. Aim 2: To implement DNA sample processing and methylation detection into a mobile phone-
manipulated microfluidic chip system. Aim 3: To test a prototype ESCC diagnostic strategy integrating the DNA
methylation detection system with the swallowable sponge for sample collection. UH3 PHASE: Aim 1: To
improve the cost-effectiveness and robustness of the methylation diagnostic system for use in LMICs. Aim 2:
To develop a method for ambient chip storage and perform on-chip QC tests to verify assay functionality. Aim
3: To conduct an ESCC diagnostic trial in Uganda using our point-of-care strategy.
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DOI:
10.1002/cncr.30837
发表时间:
2017-10-15
期刊:
Cancer
影响因子:
6.2
作者:
[Wang Z, Cheng Y, Abraham JM, Yan R, Liu X, Chen W, Ibrahim S, Schroth GP, Ke X, He Y, Meltzer SJ]
通讯作者:
Meltzer SJ
DOI:
10.1371/journal.pone.0180988
发表时间:
2017
期刊:
PloS one
影响因子:
3.7
作者:
[Song Y, Kilburn D, Song JH, Cheng Y, Saeui CT, Cheung DG, Croce CM, Yarema KJ, Meltzer SJ, Liu KJ, Wang TH]
通讯作者:
Wang TH
DOI:
10.1016/j.jcmgh.2017.02.003
发表时间:
2017-05
期刊:
Cellular and molecular gastroenterology and hepatology
影响因子:
7.2
作者:
[Liu X, Meltzer SJ]
通讯作者:
Meltzer SJ
Droplet Magnetofluidic Assay Platform for Quantitative Methylation-Specific PCR.
用于定量甲基化特异性 PCR 的液滴磁流控检测平台。
DOI:
10.1007/978-1-0716-1811-0_13
发表时间:
2022
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
作者:
[Stark,Alejandro, Trick,Alexander, Pisanic2nd,ThomasR, Wang,Tza-Huei]
通讯作者:
Wang,Tza-Huei
DOI:
10.1053/j.gastro.2017.04.046
发表时间:
2017-07
期刊:
Gastroenterology
影响因子:
29.4
作者:
[Abraham JM, Meltzer SJ]
通讯作者:
Meltzer SJ
共 8 条
Point-of-Care Diagnosis of Esophageal Cancer in LMICs
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批准号:10649166
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项目类别:
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资助金额:$62.05万
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财政年份:2023
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负责人:Stephen J Meltzer
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依托单位:
Academic-Industrial Partnership for Non-invasive Barrett's Esophagus Detection
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批准号:10456192
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资助金额:$74.78万
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财政年份:2018
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负责人:Stephen J Meltzer
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依托单位:
Academic-Industrial Partnership for Non-invasive Barrett's Esophagus Detection
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批准号:10015265
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项目类别:
-
资助金额:$74.78万
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财政年份:2018
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负责人:Stephen J Meltzer
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依托单位:
Facile screening for esophageal cancer in LMICs
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批准号:9221673
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(PQC-1) Driver Events In IBD-Associated Neoplastic Progression
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Inflammatory Bowel Disease-Associated Malignant Transformation
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批准号:8107870
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资助金额:$29.11万
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财政年份:2009
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依托单位:
The temporal epigenomic program of Barrett's neoplastic progression
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批准号:8495325
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项目类别:
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资助金额:$34.9万
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财政年份:2009
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负责人:Stephen J Meltzer
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依托单位:
Inflammatory Bowel Disease-Associated Malignant Transformation
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批准号:7929479
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项目类别:
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资助金额:$30.01万
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财政年份:2009
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负责人:Stephen J Meltzer
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依托单位:
The Role of microRNA Alterations in Barrett's Carcinogenesis
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批准号:8192921
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项目类别:
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资助金额:$33.01万
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财政年份:2009
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负责人:Stephen J Meltzer
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依托单位:
The temporal epigenomic program of Barrett's neoplastic progression
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批准号:8102924
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项目类别:
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资助金额:$34.88万
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Inflammatory Bowel Disease-Associated Malignant Transformation
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批准号:8517027
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资助金额:$27.36万
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财政年份:2009
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The Role of microRNA Alterations in Barrett's Carcinogenesis
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批准号:8310930
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项目类别:
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资助金额:$33.01万
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财政年份:2009
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依托单位:
The temporal epigenomic program of Barrett's neoplastic progression
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批准号:7726344
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资助金额:$38.18万
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财政年份:2009
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依托单位:
The temporal epigenomic program of Barrett's neoplastic progression
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批准号:8288227
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项目类别:
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资助金额:$36.17万
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财政年份:2009
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负责人:Stephen J Meltzer
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依托单位:
Inflammatory Bowel Disease-Associated Malignant Transformation
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批准号:8300953
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项目类别:
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资助金额:$29.11万
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财政年份:2009
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负责人:Stephen J Meltzer
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依托单位:
Inflammatory Bowel Disease-Associated Malignant Transformation
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批准号:7582019
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项目类别:
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资助金额:$30.01万
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财政年份:2009
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负责人:Stephen J Meltzer
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依托单位:
The Role of microRNA Alterations in Barrett's Carcinogenesis
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资助金额:$34.03万
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财政年份:2009
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The temporal epigenomic program of Barrett's neoplastic progression
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