Viral Testing Core

病毒检测核心

• 批准号: 10252432
• 负责人: Carolina Allers
• 金额: $ 39.16万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10252432
• 关键词: Acquired Immunodeficiency Syndrome; Animals; Antigens; Biological Assay; Breeding; Cercopithecine Herpesvirus 1; Chickenpox; Cytomegalovirus; Data; Detection; Development; Diagnostic Services; Frequencies; Funding; Future; Grant; Infant; Laboratories; Lymphocryptovirus; Maintenance; Microbiology; Mission; Parents; Pathogen detection; Performance; Primates; Research; Research Personnel; Research Support; Rhadinovirus; Rhesus; Rivers; SIV; Sampling; Shipping; Simian Foamy Virus; Simian Retroviruses; Simian virus 40; Site; Source; Subgroup; Surveillance Program; Techniques; Testing; Time; United States National Institutes of Health; Validation; Validity of Results; Veterinary Medicine; Viral; Viral Physiology; Virus; animal colony; cost; diagnostic assay; interest; nonhuman primate; offspring; pathogen; performance tests; programs; quality assurance; screening; success; targeted agent; working group

Project Summary/Abstract - Viral Testing Core Continuous, accurate surveillance for agents of concern is an essential requirement for maintenance of a SPF breeding colony. In 2009 we transitioned from testing at off-site laboratories to performing all screening assays at the Tulane National Primate Research Center's (TNPRC) Pathogen Detection and Quantification Core (PDQC). The PDQC is administratively located in the Division of Microbiology and was established at the TNPRC in 2004. The primary mission of the PDQC is to provide diagnostic services to the Division of Veterinary Medicine for establishing and maintaining SPF nonhuman primate colonies. A secondary mission is to provide research support to investigators. The development of onsite viral testing capabilities has resulted in reduced costs, eliminated shipping of samples, reduced turnaround time, and provided a platform for easy expansion for detection of additional agents. Quality assurance of the TNPRC SPF surveillance program is supported by the participation of the PDQC in proficiency panels testing from the Pathogen Detection Working Group (PDWG), a subgroup of the Breeding Colony Management Consortium (BCMC) in the NIH Nonhuman Primate Research Consortium (NHPRC), and from the External Quality Assurance Program Oversight Laboratory (EQAPOL) Luminex program. This section of the application focuses on the function of the Viral Testing Core, techniques used for identification and detection of the target viruses, assay performance and validation, and current and future expansion of testing capabilities. The Aim of this core is to assure the SPF status of the colony through continued viral testing of existing breeding colony animals and all offspring. The SPF4 colony will be tested a minimum of twice each year for all four target viruses: Type D simian retrovirus (SRV), simian T-lymphotropic virus (STLV), simian immunodeficiency virus (SIV), and herpes B virus (BV) for SPF4. The eSPF colony will be tested at the same frequency for rhesus rhadinovirus (RRV), simian virus 40 (SV40), simian foamy virus (SFV), cytomegalovirus (CMV), simian varicella virus (SVV), and lymphocryptovirus (LCV) in addition to the SPF4 agents. Infants derived from SPF4 parents for inclusion in the eSPF colony will require quarterly testing. Characterization of the colony beyond the targeted agents supported by the U42 grant will be funded through other sources and require the development and use of additional assays for other pathogens of potential interest.

项目总结/摘要-病毒检测核心 持续、准确地监测所关注的病原体是维持SPF的基本要求 繁殖群体2009年，我们从在非现场实验室进行检测过渡到进行所有筛查检测 在杜兰国家灵长类动物研究中心（TNPRC）的病原体检测和定量中心， （PDQC）。PDQC在行政上位于微生物学部，并在TNPRC成立 2004年PDQC的主要使命是为兽医部门提供诊断服务 用于建立和维持SPF非人灵长类动物群体。第二个使命是提供研究 支持调查员。现场病毒检测能力的发展降低了成本， 消除了样品运输，缩短了周转时间，并提供了一个易于扩展的平台， 检测其他试剂。TNPRC SPF监测计划的质量保证得到以下方面的支持： PDQC参与病原体检测工作组（PDWG）的熟练度小组测试，a NIH非人灵长类研究中的繁殖群管理联盟（BCMC）亚组 联合体（NHPRC）和外部质量保证计划监督实验室（EQAPOL） Luminex计划。本节的应用程序侧重于功能的病毒检测核心，技术 用于鉴定和检测靶病毒、检测试剂盒性能和验证，以及当前和 未来的测试能力。 该核心的目的是通过对现有育种进行持续的病毒检测，确保殖民地的SPF状态 群体动物和所有后代。对于所有四个目标，SPF 4菌落每年至少检测两次 病毒：D型猿猴逆转录病毒（SRV）、猿猴嗜T淋巴细胞病毒（STLV）、猿猴免疫缺陷病毒 (SIV)和用于SPF 4的疱疹B病毒（BV）。将以与恒河猴相同的频率检测eSPF菌落 杆状病毒（RRV）、猿猴病毒40（SV 40）、猿猴泡沫病毒（SFV）、巨细胞病毒（CMV）、猿猴水痘 病毒（SVV）和淋巴隐病毒（LCV）。来自父母SPF 4的婴儿 包含在eSPF菌落中的细菌将需要每季度进行一次检测。目标以外的殖民地表征 由U42赠款支持的代理商将通过其他来源获得资金，并要求开发和使用 其他潜在病原体的额外检测。