Viral Testing Core

病毒检测核心

• 批准号: 10331080
• 负责人: Carolina Allers
• 金额: $ 22.85万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-09 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10331080
• 关键词: Acquired Immunodeficiency Syndrome; Animals; Antigens; Biological Assay; Breeding; Cercopithecine Herpesvirus 1; Data; Detection; Development; Diagnostic Services; Future; Grant; Laboratories; Macaca; Maintenance; Microbiology; Mission; Pathogen detection; Performance; Primates; Research; Research Personnel; Research Support; Rivers; SIV; Sampling; Shipping; Simian Retroviruses; Site; Subgroup; Surveillance Program; Techniques; Testing; Time; United States National Institutes of Health; Validation; Validity of Results; Veterinary Medicine; Viral; Viral Physiology; Virus; animal colony; cost; diagnostic assay; interest; nonhuman primate; offspring; pathogen; performance tests; programs; quality assurance; screening; success; targeted agent; working group

Project Summary/Abstract - Viral Testing Core Continuous, accurate surveillance for agents of concern is an essential requirement for maintenance of a SPF breeding colony. In 2009 we transitioned from testing at off-site laboratories to performing all screening assays at the Tulane National Primate Research Center’s (TNPRC) Pathogen Detection and Quantification Core (PDQC). The PDQC is administratively located in the Division of Microbiology and was established at the TNPRC in 2004. The primary mission of the PDQC is to provide diagnostic services to the Division of Veterinary Medicine for establishing and maintaining SPF nonhuman primate colonies. A secondary mission is to provide research support to investigators. The development of onsite viral testing capabilities has resulted in reduced costs, eliminated shipping of samples, reduced turnaround time, and provided a platform for easy expansion for detection of additional agents. Quality assurance of the TNPRC SPF surveillance program is supported by the participation of the PDQC in proficiency panels testing from the Pathogen Detection Working Group (PDWG) of the NIH Nonhuman Primate Research Consortium (NHPRC) and the External Quality Assurance Program Oversight Laboratory (EQAPOL) Luminex program. This section of the application focuses on the function of the Viral Testing Core, techniques used for identification and detection of the target viruses, assay performance and validation, and current and future expansion of testing capabilities. The Aim of this core is to assure the SPF status of the colony through continued viral testing of existing breeding colony animals and all offspring. The SPF4 colony will be tested a minimum of twice each year for all four target viruses: Type D simian retrovirus (SRV), simian T-lymphotropic virus (STLV), simian immunodeficiency virus (SIV), and herpes B virus (BV) for SPF4. Characterization of the colony beyond the targeted agents supported by the U42 grant will continue through the development and use of additional assays for other pathogens of potential interest.

项目摘要/摘要-病毒测试核心 持续、准确地监测受关注的制剂是维持SPF的基本要求 繁殖地。2009年，我们从在非现场实验室进行测试过渡到进行所有筛查分析 在杜兰国家灵长类研究中心(TNPRC)的病原体检测和量化核心 (PDQC)。PDQC设在微生物学处，成立于TNPRC 在2004年。兽医质量控制中心的主要任务是向兽医司提供诊断服务 用于建立和维护SPF非人类灵长类动物群体。次要任务是提供研究 对调查人员的支持。现场病毒检测能力的发展降低了成本， 消除了样品运输，缩短了周转时间，并提供了易于扩展的平台 检测其他代理。TNPRC SPF监测计划的质量保证由 PDQC参与由病原体检测工作组(PDWG)进行的能力小组测试 NIH非人灵长类研究联盟(NHPRC)和外部质量保证计划 监督实验室(EQAPOL)Luminex计划。应用程序的这一部分重点介绍 病毒检测核心、用于识别和检测目标病毒的技术、检测性能和 验证，以及测试能力的当前和未来扩展。 这个核心的目的是通过对现有育种进行持续的病毒测试来确保群体的SPF状态 群居动物和所有后代。对于所有四个目标，SPF4菌落每年将至少接受两次测试 病毒：D型猴逆转录病毒(SRV)、猴T淋巴细胞白血病病毒(STLV)、猴免疫缺陷病毒 (SIV)和SPF4的疱疹B病毒(BV)。所支持的靶向制剂以外的菌落特征 将继续通过开发和使用其他检测其他病原体的方法。 潜在的兴趣。