"Role of the cytotoxin, CptA, from the emerging bacterial pathogen Sneathia vaginalis, in pathogenesis"
“来自新兴细菌病原体阴道球菌的细胞毒素 CptA 在发病机制中的作用”
基本信息
- 批准号:10593631
- 负责人:
- 金额:$ 23.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-11-04 至 2024-10-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAmino AcidsAnaerobic BacteriaAnimalsAntibodiesAntibody ResponseBacteremiaBindingBiological ModelsBiologyBlood specimenClinical MicrobiologyCulture-independent methodsCytotoxinDNADataDegenerative polyarthritisDetectionDevelopmentEndometritisEnzyme-Linked Immunosorbent AssayEpithelial CellsEtiologyExhibitsFemaleFetal DeathFetal MembranesGenesGoalsHealthy People 2020HistophilusHumanImmuneImmune responseImmunoglobulin AImmunoglobulin GImmunoglobulin MIn VitroInbred BALB C MiceIncidenceInfectionInvadedMeasurementMediatingMembraneMeningitisMethodsModelingMothersMusNamesNaturePathogenesisPeripheralPhylogenetic AnalysisPilot ProjectsPlacentaPlasmaPlayPregnancyPregnancy ComplicationsPregnancy OutcomePremature BirthProteinsRecombinantsRoleRouteRuptureSamplingSepsisSerumSpecimenSurfaceSurveysSwabTerm BirthTestingTimeToxinUmbilical Cord BloodUnited States National Institutes of HealthUrethritisUterine cavityUterusVaginaVirulence Factorsdesignemerging pathogenfetalhuman subjectin vivoinfant deathinfant morbidity/mortalitymicrobiome researchmouse modelmutantneonatal morbidityneonatenovelpathogenpathogenic bacteriapregnantpreterm premature rupture of membranesreceptorreproductive tracttrophoblastvaginal microbiota
项目摘要
PROJECT SUMMARY:
Sneathia vaginalis (Sv) is emerging as a pathogen that is significantly associated with preterm birth and can
cause numerous infections including amnionitis, osteoarthritis, meningitis, bacteremia, and urethritis. Due to its
fastidious nature, it has only recently been recognized as a pathogen through the use of DNA-based methods.
Because it has been under-recognized as a pathogen, the biology and the pathogenesis of S. vaginalis remain
almost entirely uncharacterized. We have identified a hemolytic and cytopathogenic toxin produced by S.
vaginalis that we have named CptA. Antiserum against CptA abrogates traversal of Sv across intact human fetal
membranes suggesting that the toxin plays a role in pathogenesis. This project has three main goals. The first
is to assess the maternal and fetal antibody response to CptA during natural colonization/infection. The second
is to establish the role of CptA in traversal of and damage to fetal membranes by Sv. The third is to determine
whether the association between Sv and preterm birth is causal by developing a mouse model of Sv infection
during pregnancy. pathogenesis Characterization of this novel pore-forming toxin will break ground in our
understanding of this emerging pathogen.
项目概要:
迷走神经鞘炎(Sv)是一种与早产显著相关的病原体,
引起许多感染,包括淋病、骨关节炎、脑膜炎、菌血症和尿道炎。由于其
由于其挑剔的性质,直到最近才通过使用基于DNA的方法被确认为病原体。
由于其作为一种病原体的认识不足,目前对该菌的生物学特性和致病机理的研究还处于起步阶段。迷走神经残余
几乎完全没有特征。我们已经鉴定出由S.
我们称之为CptA。抗CptA抗血清阻断Sv穿过完整人胎儿的能力
这表明毒素在发病机制中起作用。该项目有三个主要目标。第一
评估自然定殖/感染期间母体和胎儿对CptA的抗体应答。第二
目的是确定CptA在Sv穿过和损伤胎膜中的作用。三是确定
通过建立小鼠Sv感染模型,研究Sv与早产之间的关系是否是因果关系
孕期这种新的成孔毒素的发病机制特征将在我们的研究中开辟新的领域。
了解这种新出现的病原体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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KIMBERLY Kay JEFFERSON其他文献
KIMBERLY Kay JEFFERSON的其他文献
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{{ truncateString('KIMBERLY Kay JEFFERSON', 18)}}的其他基金
The Virulome of Infectious Preterm Birth (Project 2; PI: Jefferson)
传染性早产的病毒组(项目 2;负责人:Jefferson)
- 批准号:
8655803 - 财政年份:2014
- 资助金额:
$ 23.29万 - 项目类别:
The Virulome of Infectious Preterm Birth (Project 2; PI: Jefferson)
传染性早产的病毒组(项目 2;负责人:Jefferson)
- 批准号:
8354916 - 财政年份:2012
- 资助金额:
$ 23.29万 - 项目类别:
Post-transcriptional regulation of biofilm formation S. aureus
生物膜形成的转录后调控金黄色葡萄球菌
- 批准号:
8147498 - 财政年份:2010
- 资助金额:
$ 23.29万 - 项目类别:
Post-transcriptional regulation of biofilm formation S. aureus
生物膜形成的转录后调控金黄色葡萄球菌
- 批准号:
7391713 - 财政年份:2007
- 资助金额:
$ 23.29万 - 项目类别:
Post-transcriptional regulation of biofilm formation S. aureus
生物膜形成的转录后调控金黄色葡萄球菌
- 批准号:
7796865 - 财政年份:2007
- 资助金额:
$ 23.29万 - 项目类别:
Post-transcriptional regulation of biofilm formation S. aureus
生物膜形成的转录后调控金黄色葡萄球菌
- 批准号:
7599211 - 财政年份:2007
- 资助金额:
$ 23.29万 - 项目类别:
Post-transcriptional regulation of biofilm formation S. aureus
生物膜形成的转录后调控金黄色葡萄球菌
- 批准号:
7210257 - 财政年份:2007
- 资助金额:
$ 23.29万 - 项目类别:
Post-transcriptional regulation of biofilm formation S. aureus
生物膜形成的转录后调控金黄色葡萄球菌
- 批准号:
8038279 - 财政年份:2007
- 资助金额:
$ 23.29万 - 项目类别:
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