Role of LH-induced cell migration and cofilin dephosphorylation in ovulation

LH 诱导的细胞迁移和丝动蛋白去磷酸化在排卵中的作用

基本信息

项目摘要

Project Abstract: In the ovary, luteinizing hormone (LH) signals through the luteinizing hormone receptor (LHR) to initiate the transition from preovulatory follicle to corpus luteum. Essential to this transition are the movement of the oocyte to the site of ovulation, basal lamina breakdown, and reorganization of the granulosa cells to form the corpus luteum. LHR-expressing cells migrate inwards in response to LH and at later time points after LH stimulation, the basal lamina is pulled inwards in regions of high HA-LHR expression. These results have led to the hypothesis that LH-induced granulosa cell migration aids in regulating the transition from preovulatory follicle to corpus luteum. Previous studies in isolated granulosa cells have indicated that LH signaling disrupts actin to allow cytoskeletal rearrangement and induces granulosa cell motility. One potential mediator of these actions is cofilin, an actin-depolymerizing protein. Cofilin activity is required for directional cell motility in multiple cell types, and expression of a dominant-negative form of the protein in granulosa cell inhibits LH-induced actin reorganization. However, the role of cofilin dephosphorylation in vivo has yet to be investigated. This project investigates the hypothesis that LH induces cell migration through dephosphorylation of cofilin, and that the migration pulls the basal lamina inward to aid in rupture during ovulation and reorganizes the cells to begin corpus luteum formation. In aim 1, live-tissue confocal and two-photon microscopy will be used to analyze granulosa cell migration in intact follicles and determine how granulosa cell migration contributes to basal lamina invaginations and ovulation. In aim two, a novel mouse line that expresses a dominant-negative form of cofilin will be used to explore the hypothesis that cofilin dephosphorylation regulates granulosa cell migration. These studies will provide novel information about the regulation of ovulation and could lead to clinical treatments for anovulatory diseases.
项目摘要: 在卵巢中,黄体生成素 (LH) 通过黄体生成素受体 (LHR) 发出信号,启动 从排卵前卵泡到黄体的转变。这种转变的关键是卵母细胞的运动 到达排卵、基底层破裂和颗粒细胞重组形成语料库的部位 黄体。 LHR 表达细胞响应 LH 向内迁移,并在 LH 刺激后的较晚时间点迁移, HA-LHR 高表达区域的基底层被向内拉。这些结果导致 LH 诱导的颗粒细胞迁移有助于调节从排卵前卵泡到排卵前卵泡的转变。 黄体。先前对分离颗粒细胞的研究表明,LH 信号传导会破坏肌动蛋白,从而 允许细胞骨架重排并诱导颗粒细胞运动。这些行动的一个潜在调解者是 cofilin,一种肌动蛋白解聚蛋白。丝动蛋白活性是多种细胞类型中定向细胞运动所必需的, 颗粒细胞中该蛋白的显性失活形式的表达抑制 LH 诱导的肌动蛋白 重组。然而,肌动蛋白丝切蛋白去磷酸化在体内的作用仍有待研究。这个项目 研究了 LH 通过 cofilin 去磷酸化诱导细胞迁移的假设,并且 迁移将基底层向内拉,以帮助排卵期间破裂并开始重组细胞 黄体形成。在目标 1 中,将使用活组织共焦和双光子显微镜来分析 完整卵泡中的颗粒细胞迁移并确定颗粒细胞迁移如何促进基底层 内陷和排卵。目标二是表达丝切蛋白显性失活形式的新型小鼠品系 将用于探索肌动蛋白丝切蛋白去磷酸化调节颗粒细胞迁移的假设。这些 研究将提供有关排卵调节的新信息,并可能导致临床治疗 无排卵疾病。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle†.
黄体生成素刺激小鼠排卵前卵泡内壁颗粒细胞的侵入。
  • DOI:
    10.1093/biolre/ioad142
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    3.6
  • 作者:
    Owen,CorieM;Jaffe,LaurindaA
  • 通讯作者:
    Jaffe,LaurindaA
Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle.
黄体生成素刺激小鼠排卵前卵泡内壁颗粒细胞的侵入。
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Corie Marie Owen其他文献

Corie Marie Owen的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Corie Marie Owen', 18)}}的其他基金

Role of LH-induced cell migration and cofilin dephosphorylation in ovulation
LH 诱导的细胞迁移和丝动蛋白去磷酸化在排卵中的作用
  • 批准号:
    10386571
  • 财政年份:
    2022
  • 资助金额:
    $ 4.19万
  • 项目类别:

相似海外基金

University of Aberdeen and Vertebrate Antibodies Limited KTP 23_24 R1
阿伯丁大学和脊椎动物抗体有限公司 KTP 23_24 R1
  • 批准号:
    10073243
  • 财政年份:
    2024
  • 资助金额:
    $ 4.19万
  • 项目类别:
    Knowledge Transfer Partnership
Role of Natural Antibodies and B1 cells in Fibroproliferative Lung Disease
天然抗体和 B1 细胞在纤维增生性肺病中的作用
  • 批准号:
    10752129
  • 财政年份:
    2024
  • 资助金额:
    $ 4.19万
  • 项目类别:
CAREER: Next-generation protease inhibitor discovery with chemically diversified antibodies
职业:利用化学多样化的抗体发现下一代蛋白酶抑制剂
  • 批准号:
    2339201
  • 财政年份:
    2024
  • 资助金额:
    $ 4.19万
  • 项目类别:
    Continuing Grant
Isolation and characterisation of monoclonal antibodies for the treatment or prevention of antibiotic resistant Acinetobacter baumannii infections
用于治疗或预防抗生素耐药鲍曼不动杆菌感染的单克隆抗体的分离和表征
  • 批准号:
    MR/Y008693/1
  • 财政年份:
    2024
  • 资助金额:
    $ 4.19万
  • 项目类别:
    Research Grant
Discovery of novel nodal antibodies in the central nervous system demyelinating diseases and elucidation of the mechanisms through an optic nerve demyelination model
发现中枢神经系统脱髓鞘疾病中的新型节点抗体并通过视神经脱髓鞘模型阐明其机制
  • 批准号:
    23K14783
  • 财政年份:
    2023
  • 资助金额:
    $ 4.19万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Elucidation of the mechanisms controlling the physicochemical properties and functions of supercharged antibodies and development of their applications
阐明控制超电荷抗体的理化性质和功能的机制及其应用开发
  • 批准号:
    23KJ0394
  • 财政年份:
    2023
  • 资助金额:
    $ 4.19万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Developing first-in-class aggregation-specific antibodies for a severe genetic neurological disease
开发针对严重遗传神经系统疾病的一流聚集特异性抗体
  • 批准号:
    10076445
  • 财政年份:
    2023
  • 资助金额:
    $ 4.19万
  • 项目类别:
    Grant for R&D
PLA2G2D Antibodies for Cancer Immunotherapy
用于癌症免疫治疗的 PLA2G2D 抗体
  • 批准号:
    10699504
  • 财政年份:
    2023
  • 资助金额:
    $ 4.19万
  • 项目类别:
Genetic adjuvants to elicit neutralizing antibodies against HIV
基因佐剂可引发抗艾滋病毒中和抗体
  • 批准号:
    10491642
  • 财政年份:
    2023
  • 资助金额:
    $ 4.19万
  • 项目类别:
Novel Immunogens to Elicit Broadly Cross-reactive Antibodies That Target the Hemagglutinin Head Trimer Interface
新型免疫原可引发针对血凝素头三聚体界面的广泛交叉反应抗体
  • 批准号:
    10782567
  • 财政年份:
    2023
  • 资助金额:
    $ 4.19万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了