SBIR Fast Track: Development of a High-throughput Magnetic Cytometer for Single Cell Sorting
SBIR 快速通道:开发用于单细胞分选的高通量磁力细胞仪
基本信息
- 批准号:10599601
- 负责人:
- 金额:$ 99.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-04-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAlgorithmsB-LymphocytesBasic ScienceBiological AssayBiological SciencesBloodBlood CellsBlood specimenBudgetsBusinessesCell Culture TechniquesCell LineCell SeparationCell SurvivalCellsCellular StructuresClinicalComplexComputer softwareConsumptionDetectionDevelopmentDevicesGoalsHealthcareHourInnovation CorpsInterviewLegal patentMagnetismMechanicsMicrofluidicsMicromanipulationMolecular AnalysisMovementNational Institute of General Medical SciencesNeoplasm Circulating CellsOpticsPatientsPhasePopulationPopulation HeterogeneityProtocols documentationRecoveryResearchResearch Project GrantsResolutionSamplingSemiconductorsSmall Business Innovation Research GrantSorting - Cell MovementSpeedStainsSystemTechniquesTechnologyTimeTranslational ResearchUnited States National Institutes of HealthValidationantigen-specific T cellsbasecell fixationclinical applicationcommercializationcost effectivedesignfetus cellfluorescence imagingimaging systeminnovationinstrumentationinterestmicrosensormonoclonal antibody productionnext generationnoveloptical imagingparticleprecision medicineprocessing speedprotocol developmentprototyperapid growthsample fixationsensorsingle cell analysissingle-cell RNA sequencingsoftware developmentstem cellstooltumoruser-friendlywaiver
项目摘要
Project Summary
This Small Business Innovation Research project aims to develop a high-throughput easy-to-use cell sorter
MagiCyte™ to rapidly and precisely sort intact and viable rare cells at single-cell resolution.
Single-cell sorting from a heterogeneous cell population has been essential to life science applications such as
monoclonal antibody production and cell line development. Recently, with the rapid growth of precision
medicine, there has also been a strong demand for single-cell sorting technologies for rare cells, such as
circulating tumor cells (CTCs), circulating fetal cells, stem cells and antigen-specific T- or B-cells. However,
collecting such rare cells at single-cell resolution to meet the requirements of single-cell analysis is not feasible
for conventional single-cell sorting techniques. Micromanipulation and serial dilution are time consuming and
low-throughput, while fluorescent-activated cell sorting (FACS) suffers from remarkable cell loss and is
inefficient in isolating rare cells. In addition, many such techniques require cell fixation and/or fluorescent
staining, which decreases cell viability and thus prohibits many downstream molecular analyses, as well as
and cell culture. As such, there is an unaddressed need for high throughput single-cell sorting of rare cells.
We aim to develop a high-throughput chip-based cytometer MagiCyte™ to rapidly isolate, identify and sort
single intact and viable target cells from heterogeneous cell population. The core component of MagiCyte is a
semiconductor chip MagiChip™ which is used for single cell identification and single cell sorting. In this fast-
track project, we will first develop the control software and demonstrate automated single-cell sorting; In Phase
II, we will develop the next-generation MagiChip, build the MagiCyte beta product and validate the system on
clinical samples with our collaborators.
项目摘要
这个小型企业创新研究项目旨在开发一种高通量、易于使用的细胞分选器
MagiCyte™可在单细胞分辨率下快速、准确地分选完整和可存活的稀有细胞。
从异质细胞群中进行单细胞分离对于生命科学的应用是必不可少的,例如
单抗的产生和细胞系的建立。近年来,随着精确度的快速增长
在医学方面,对稀有细胞的单细胞分选技术也有强烈的需求,例如
循环中的肿瘤细胞(CTCs)、循环中的胎儿细胞、干细胞和抗原特异性T或B细胞。然而,
以单细胞分辨率收集这种稀有细胞来满足单细胞分析的要求是不可行的
用于传统的单细胞分选技术。微操作和连续稀释很耗时,而且
低吞吐量,而荧光激活细胞分选(FACS)遭受显著的细胞损失和
在分离稀有细胞方面效率低下。此外,许多这样的技术需要细胞固定和/或荧光
染色,这会降低细胞活力,从而阻止许多下游分子分析,以及
和细胞培养。因此,存在对稀有细胞的高通量单细胞分选的未解决的需求。
我们的目标是开发一种基于芯片的高通量细胞分析仪MagiCyte™,用于快速分离、鉴定和分选
来自异种细胞群体的单个完整且有活力的靶细胞。MagiCyte的核心组件是
半导体芯片MagiChip™,用于单细胞识别和单细胞分类。在这么快的速度下-
Track项目,我们将首先开发控制软件并演示自动单细胞分选;在
第二,我们将开发下一代MagiChip,构建MagiCyte测试版产品,并在
与我们的合作者一起提供临床样本。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Peng Liu其他文献
Geodynamic setting of Late Cretaceous Sn–W mineralization in southeastern Yunnan and northeastern Vietnam
云南东南部和越南东北部晚白垩世Sn-W矿化的地球动力学背景
- DOI:
10.1016/j.sesci.2016.12.001 - 发表时间:
2016-12 - 期刊:
- 影响因子:2
- 作者:
Yanbo Cheng;Jingwen Mao;Peng Liu - 通讯作者:
Peng Liu
Peng Liu的其他文献
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{{ truncateString('Peng Liu', 18)}}的其他基金
SBIR Fast Track: Development of a High-throughput Magnetic Cytometer for Single Cell Sorting
SBIR 快速通道:开发用于单细胞分选的高通量磁力细胞仪
- 批准号:
10385619 - 财政年份:2022
- 资助金额:
$ 99.97万 - 项目类别:
SBIR Fast Track: Development of a High-throughput Magnetic Cytometer for Single Cell Sorting
SBIR 快速通道:开发用于单细胞分选的高通量磁力细胞仪
- 批准号:
10703514 - 财政年份:2022
- 资助金额:
$ 99.97万 - 项目类别:
Computational Models for Reactivity and Selectivity in Transition Metal-Catalyzed Olefin Functionalization
过渡金属催化烯烃官能化反应性和选择性的计算模型
- 批准号:
10242139 - 财政年份:2018
- 资助金额:
$ 99.97万 - 项目类别:
Computational Models for Reactivity and Selectivity in Transition Metal-Catalyzed Olefin Functionalization
过渡金属催化烯烃官能化反应性和选择性的计算模型
- 批准号:
9769078 - 财政年份:2018
- 资助金额:
$ 99.97万 - 项目类别:
Development of A Novel Magnetic Exosomal Assay for Diagnosis of Diseases with Impaired Vascular Integrity
开发一种用于诊断血管完整性受损疾病的新型磁性外泌体测定法
- 批准号:
9410664 - 财政年份:2017
- 资助金额:
$ 99.97万 - 项目类别:
Differential regulation of autoimmune arthritis by monocyte subsets
单核细胞亚群对自身免疫性关节炎的差异调节
- 批准号:
9114096 - 财政年份:2012
- 资助金额:
$ 99.97万 - 项目类别:
Differential regulation of autoimmune arthritis by monocyte subsets
单核细胞亚群对自身免疫性关节炎的差异调节
- 批准号:
8345744 - 财政年份:2012
- 资助金额:
$ 99.97万 - 项目类别:
Differential regulation of autoimmune arthritis by monocyte subsets
单核细胞亚群对自身免疫性关节炎的差异调节
- 批准号:
8708501 - 财政年份:2012
- 资助金额:
$ 99.97万 - 项目类别:
Differential regulation of autoimmune arthritis by monocyte subsets
单核细胞亚群对自身免疫性关节炎的差异调节
- 批准号:
8518169 - 财政年份:2012
- 资助金额:
$ 99.97万 - 项目类别:
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