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Eco-evolutionary drivers of clonal dynamics during UV-induced skin carcinogenesis (PQ3)

紫外线诱发皮肤癌过程中克隆动力学的生态进化驱动因素（PQ3）

基本信息

批准号：
10599889
负责人：
Joel Brown
金额：
$ 62.72万
依托单位：
H. LEE MOFFITT CANCER CTR & RES INST
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10599889
关键词：
Actinic keratosis Architecture Binding Biological Models Cancer Center Carcinogens Carcinoma Cell division Cells Characteristics Chronic Clinical Clonal Evolution Development Ecology Environment Epithelium Event Genetic Genetic Transcription Genomics Goals Heritability Heterogeneity Human Individual Investigation Lesion Ligands Link Malignant - descriptor Malignant Neoplasms Mathematics Measures Mediating Modeling Molecular Mutation Normal tissue morphology Oncology Pathogenicity Phase Proliferating Running Skin Skin Cancer Skin Carcinogenesis Skin Carcinoma Stress Testing Time Tissues Transplantation UV Radiation Exposure UV induced Ultraviolet Rays Variant Work cancer initiation carcinogenesis driver mutation experience fitness in vivo mathematical model molecular dynamics multidisciplinary novel permissiveness premalignant pressure quantitative imaging receptor single cell sequencing skin squamous cell carcinoma trait tumor tumor microenvironment

项目摘要

ABSTRACT Cancers have readily-defined characteristics often referred to as “hallmarks”. Nevertheless, the question of how the sequence of cancer development progresses -- from normal tissue to carcinogen-damaged tissue to precancerous lesion and finally to malignant tumors – remains unanswered. Classically, these steps are attributed to the sequential acquisition of discrete genetic events such as driver mutations. However, in humans, the clonal dynamics governing cancer development happen over years, remain largely invisible even in model systems, and have been difficult to link to specific molecular changes. This rubric fails to account for clonal dynamics in the context of tissue architecture and fails to explain the consequences of large numbers of mutations present in normal tissue. Our long-term goal is to apply ecological and evolutionary principles to cancer initiation and development in order to test whether the hallmarks of cancer are acquired in three distinct phases each with distinct selective pressures and manifestations of cell competition and cooperation. Nowhere is this more accessible to investigation than in skin. For skin carcinomas, the most important carcinogen is ultraviolet radiation. Cutaneous squamous cell carcinoma (cuSCC) has the most tractable and clinically well-characterized progression sequence of any human cancer, from normal tissue, to a distinct precancerous lesion (the actinic keratosis), to invasive carcinoma. Therefore, it is ideal for establishing an eco- evolutionary paradigm of cancer initiation and development with respect to modelling clonal dynamics, genetic composition and the dynamics of molecular traits. Our central hypothesis is that cancer initiation and development occurs in three phases, each with specific and recognizable clonal dynamics. In the first phase, tissue disruption from UV exposure provides a permissive environment where extrinsically-driven mechanisms allow for some clones to experience unusually long runs of cell division and turnover. This greatly increases the variance among clone sizes with larger clones accumulating greater heritable variation. The second phase sees the emergence of intrinsic mechanisms where mutations that confer a competitive advantage allow for clonal selection with directed expansion of some clones at the expense of others. In the third phase, one or several clones escape local tissue control, acquire a distinct fitness function, and form tumors. Within the emerging tumor microenvironments, selection pressures will promote ecological and molecular diversification of the malignant clade (or clades). Our approach uses novel combinations of serial in-vivo quantitative imaging, mathematical modeling, and deep single-cell molecular interrogation to discern the ecological and molecular drivers of clonal dynamics, cell-to- cell competition and cooperation, and clonal evolution, producing a fundamentally unprecedented view of cancer initiation.

摘要