Portable Scaled Sample Preparation Device for Concentration and Recovery of Bacterial Contaminants

用于细菌污染物浓缩和回收的便携式规模样品制备装置

• 批准号: 10603220
• 负责人: Xiaoli Su
• 金额: $ 25.96万
• 依托单位: VIC FOUNDRY, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-25 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10603220
• 关键词: Adhesives; Agriculture; Bacteria; Biological Assay; Buffers; Cells; Cessation of life; Chemicals; Collaborations; Colony-forming units; Consumption; Decontamination; Detection; Device Designs; Devices; Disease Outbreaks; Disinfectants; Early Diagnosis; Electrolyses; Emergency department visit; Escherichia coli; Escherichia coli O157:H7; Food; Food Contamination; Food Safety; Goals; Guidelines; Hawaii; Health Care Costs; Hospitalization; Hour; Immunoassay; Irrigation; Legal patent; Letters; Link; Liquid substance; Mediating; Methods; Microbubbles; Modeling; Modernization; Molecular; Nucleic Acid Amplification Tests; Phase; Preparation; Process; Public Health; Recovery; Recreation; Research; Risk; Salmonella typhimurium; Sample Size; Sampling; Site; Small Business Innovation Research Grant; Techniques; Technology; Testing; Time; United States; Universities; Water; Work; commercial prototype; commercialization; detection limit; detection platform; drinking water; farmer; field study; foodborne infection; improved; isothermal amplification; manufacture; microbial; microorganism; nonpathogenic Escherichia coli; pathogen; pathogenic bacteria; phase 1 study; portability; prototype; safety testing; scale up; wasting; water quality; water sampling; waterborne infection

Project Summary The overall goal of this SBIR project is to establish the technical feasibility for commercialization of a patent-pending sample preparation technology that concentrates and recovers microbial contaminants from appropriately scaled water samples. The proposed product is affordable, versatile, reusable, portable, and automated device with no moving parts, suitable for on-site or in-field sample preparation. Recovered eluents resulted from the sample preparation can be delivered into microscale tests. Phase I Specific aims are to evaluate the feasibility of developing a portable automated sample preparation device that can be scaled up to process larger volume samples, develop ready-to-use buffer concentrates for improving concentration and recovery of bacteria, and obtain a proof of concept for detecting low levels of bacterial contaminants, by combination of the proposed sample preparation device and rapid molecular test. In addition to outbreaks linked to recreational and drinking water, produce and food contaminations by pathogens from irrigation and other agricultural water are an increasing concern. Early detection and routine surveillance is of paramount importance for effective management of water or foodborne infectious diseases. However, current standard practices are time consuming, largely due to the requirement of extensive sample preparation. The proposed technology would be able to overcome major limitations of conventional sample preparation methods, enable detection of low levels of bacteria in different water or liquid sample types, and expand the widespread and meaningful use of rapid, portable detection platforms.

项目摘要 该SBIR项目的总体目标是建立以下技术可行性： 正在申请专利的样品制备技术的商业化， 从适当规模的水样中回收微生物污染物。拟议 产品是经济实惠、多功能、可重复使用、便携式和自动化的设备，没有移动部件， 适用于现场或现场样品制备。从样品中获得的洗脱液 制备可以交付到微型测试。第一阶段的具体目标是评估 开发可按比例放大的便携式自动样品制备装置的可行性 为了处理更大体积的样品，开发即用型缓冲液浓缩物， 细菌的浓缩和回收，并获得用于检测低水平细菌的概念验证。 细菌污染物，通过结合所提出的样品制备装置和快速 分子测试 除了与娱乐和饮用水、农产品和食品有关的疫情外， 来自灌溉和其他农业用水的病原体污染正在增加 关心早期发现和常规监测对于有效预防艾滋病毒/艾滋病至关重要。 水或食源性传染病的管理。然而，目前的标准做法 是耗时的，主要是由于需要大量的样品制备。的 所提出的技术将能够克服传统采样的主要局限性， 制备方法，能够检测不同水或液体中的低水平细菌 样品类型，并扩大广泛和有意义的使用快速，便携式检测 平台