Portable Scaled Sample Preparation Device for Concentration and Recovery of Bacterial Contaminants

用于细菌污染物浓缩和回收的便携式规模样品制备装置

• 批准号: 10603220
• 负责人: Xiaoli Su
• 金额: $ 25.96万
• 依托单位: VIC FOUNDRY, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-25 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10603220
• 关键词: Adhesives; Agriculture; Bacteria; Biological Assay; Buffers; Cells; Cessation of life; Chemicals; Collaborations; Colony-forming units; Consumption; Decontamination; Detection; Device Designs; Devices; Disease Outbreaks; Disinfectants; Early Diagnosis; Electrolyses; Emergency department visit; Escherichia coli; Escherichia coli O157:H7; Food; Food Contamination; Food Safety; Goals; Guidelines; Hawaii; Health Care Costs; Hospitalization; Hour; Immunoassay; Irrigation; Legal patent; Letters; Link; Liquid substance; Mediating; Methods; Microbubbles; Modeling; Modernization; Molecular; Nucleic Acid Amplification Tests; Phase; Preparation; Process; Public Health; Recovery; Recreation; Research; Risk; Salmonella typhimurium; Sample Size; Sampling; Site; Small Business Innovation Research Grant; Techniques; Technology; Testing; Time; United States; Universities; Water; Work; commercial prototype; commercialization; detection limit; detection platform; drinking water; farmer; field study; foodborne infection; improved; isothermal amplification; manufacture; microbial; microorganism; nonpathogenic Escherichia coli; pathogen; pathogenic bacteria; phase 1 study; portability; prototype; safety testing; scale up; wasting; water quality; water sampling; waterborne infection

Project Summary The overall goal of this SBIR project is to establish the technical feasibility for commercialization of a patent-pending sample preparation technology that concentrates and recovers microbial contaminants from appropriately scaled water samples. The proposed product is affordable, versatile, reusable, portable, and automated device with no moving parts, suitable for on-site or in-field sample preparation. Recovered eluents resulted from the sample preparation can be delivered into microscale tests. Phase I Specific aims are to evaluate the feasibility of developing a portable automated sample preparation device that can be scaled up to process larger volume samples, develop ready-to-use buffer concentrates for improving concentration and recovery of bacteria, and obtain a proof of concept for detecting low levels of bacterial contaminants, by combination of the proposed sample preparation device and rapid molecular test. In addition to outbreaks linked to recreational and drinking water, produce and food contaminations by pathogens from irrigation and other agricultural water are an increasing concern. Early detection and routine surveillance is of paramount importance for effective management of water or foodborne infectious diseases. However, current standard practices are time consuming, largely due to the requirement of extensive sample preparation. The proposed technology would be able to overcome major limitations of conventional sample preparation methods, enable detection of low levels of bacteria in different water or liquid sample types, and expand the widespread and meaningful use of rapid, portable detection platforms.

项目摘要 这个SBIR项目的总体目标是建立技术可行性 专利的样品制备技术的商业化，该技术集中于和 从适当尺度的水样品中恢复微生物污染物。提议 产品负担得起，多功能，可重复使用，便携式和自动化设备，没有活动部件， 适用于现场或场地样品制备。恢复的洗脱液是从样本中产生的 准备工作可以输送到显微镜测试中。第一阶段的特定目的是评估 开发可以扩展的便携式自动样品制备设备的可行性 要处理较大的体积样品，请开发现成的缓冲浓度以改进 细菌的浓度和恢复，并获得检测低水平的概念证明 细菌污染物，通过提议的样品制备装置的结合和快速 分子测试。 除了与娱乐和饮用水，农产品和食物有关的爆发 灌溉和其他农业水的病原体受到病原体的污染正在增加 忧虑。早期检测和常规监视对有效至关重要 水或食源性传染病的管理。但是，当前的标准实践 耗时，主要是由于需要大量样本准备。这 拟议的技术将能够克服常规样本的主要局限性 制备方法，能够检测不同水或液体中低水平的细菌 样本类型，并扩展广泛而有意义的快速检测 平台。