Rapid and low-cost PFAS detection with advanced solid-state nanopore chips

利用先进的固态纳米孔芯片进行快速、低成本的 PFAS 检测

• 批准号: 10603099
• 负责人: Zehui Xia
• 金额: $ 27.57万
• 依托单位: GOEPPERT, LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10603099
• 关键词: Address; Authorization documentation; Carbon; Characteristics; Chemical Structure; Chemicals; Collaborations; Complex; Computer software; DNA; Data Analyses; Data Collection; Detection; Development; Diameter; Electronics; Endocrine disruption; Engineering; Environment; Environmental Pollutants; Environmental Protection; Exhibits; Fire - disasters; Foundations; Genes; Health; Hepatotoxicity; Home; Human; Individual; Industrialization; Israel; Japan; Knowledge; Life; Liquid substance; Mass Fragmentography; Measurement; Measures; Membrane; Methods; Modality; Modification; Molecular Diagnosis; Monitor; Netherlands; Pharmacologic Substance; Phase; Planet Mars; Plants; Poly-fluoroalkyl substances; Privatization; Proteins; RNA; Regulation; Resolution; Sampling; Seawater; Signal Transduction; Silanes; Sodium Chloride; Soil; Solid; Solvents; Switzerland; System; Techniques; Technology; Testing; Thick; Thinness; Thyroid Gland; Translating; Work; analog; analytical tool; aqueous; authority; cost; detection limit; detection method; developmental toxicity; experience; fabrication; fetal; improved; innovation; instrument; ion mobility; landfill; manufacture; mobile sensor; nanoelectronics; nanofabrication; nanopore; nephrotoxicity; novel; operation; perfluorooctane sulfonate; perfluorooctanoic acid; portability; scale up; sensor; single molecule; small molecule; solid state; stem; symposium; technology platform; water sampling; water testing

Project Summary/Abstract Perfluoroalkyl and polyfluoroalkyl compounds (PFASs) represent a class of emerging environmental contaminants, resulting from their use in fire suppressants, and presence in landfill leachates and wastewater treatment plant effluent and solids. The presence of PFAS in the environment is a serious concern since they have exhibited hepatotoxicity, nephrotoxicity, thyroid damage, fetal and developmental toxicity, and endocrine disruption. As we begin to understand more about their application and spread and how toxic they can be, it is important and urgent to have a rapid and inexpensive way to detect PFAS. Multiple methods, including LC-MS/MS, GC-MS and ion mobility exist for sensitive and selective detection of PFAS in a variety of modalities. Unfortunately, these methods suffer from limited sample throughput, high detection limits, high operational costs, complex operation, and require knowledge of the specific PFAS chemical structure making them unsuitable for quantifying total PFASs. Several other detection techniques are emerging but are not yet themselves able to reliably determine total PFASs in samples. We seek to enable faster and lower-cost, but still reliable and robust, detection and quantification of total PFASs. This project will chart as-yet unexplored territory in develop an advanced solid-state nanopore chip to meet these urgent and comprehensive needs. Our nanopore instrument features a single-molecule precision and has been used to detect DNA, RNA, proteins and small pharmaceuticals at low concentrations from different forms of samples (liquid, solid, etc.). The approach will be enabled by a unique integration of advanced nanofabrication, high-throughput data collection and reliable data analysis software to improve the overall PFAS detection capabilities. Nanopore chips with tailored pore characteristics (diameter, thickness, coating, etc.) will be fabricated, tested and validated and their storage, stability and reusability will be systematically evaluated. We envision this platform as a first line of defense against PFAS contamination for individuals and private and public environmental protection organizations, and the engineering of an advanced solid-state nanopore chip with single molecule resolution for even broader applications.

项目总结/摘要 全氟烷基和多氟烷基化合物（PFAS）代表一类新兴的环境污染物。 污染物，由于其在灭火剂中的使用，以及在垃圾填埋场渗滤液和废水中的存在 污水处理厂污水和固体。PFAS在环境中的存在是一个严重的问题， 它们表现出肝毒性、肾毒性、甲状腺损伤、胎儿和发育毒性， 内分泌紊乱当我们开始更多地了解它们的应用和传播以及它们的毒性 因此，有一种快速、廉价的方法来检测PFAS是非常重要和紧迫的。多 方法，包括LC-MS/MS，GC-MS和离子迁移率存在的灵敏和选择性检测 PFAS以多种方式进行。不幸的是，这些方法的缺点是有限的样品通量、高通量和高通量。 检测限高，运行成本高，操作复杂，需要了解特定的PFAS 化学结构使其不适合用于定量总PFAS。其他几种检测技术 正在出现，但它们本身还不能可靠地测定样品中的总PFAS。我们寻求 能够更快、更低成本、但仍然可靠和稳健地检测和量化总PFAS。这 该项目将绘制尚未探索的领域，开发先进的固态纳米孔芯片，以满足这些 迫切而全面的需求。我们的纳米孔仪器具有单分子精度， 已被用于检测DNA，RNA，蛋白质和小药物在低浓度从不同的 样品的形式（液体、固体等）。该方法将通过独特的集成先进的 纳米纤维，高通量数据收集和可靠的数据分析软件，以提高整体 PFAS检测能力。具有定制的孔特征（直径， 厚度、涂层等）将被制造，测试和验证，其储存，稳定性 并对可重用性进行系统评估。我们把这个平台设想为 为个人、私人和公共部门防御PFAS污染 环境保护组织，和先进的固态工程 具有单分子分辨率的纳米孔芯片，适用于更广泛的应用。