F31 Microtubule function in the onset of whole-body regeneration

F31 微管在全身再生开始时的功能

• 批准号: 10607754
• 负责人: Xavier N Anderson
• 金额: $ 4.36万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10607754
• 关键词: Adult; Animals; Anterior; Cell Differentiation process; Cell Maturation; Cells; Chemicals; Cicatrix; Data; Decapitation; Ectoderm; Endoderm; Event; Eye; Fluorescent in Situ Hybridization; Fresh Water; Gene Expression; Genes; Genetic Epistasis; Germ Layers; Goals; Growth; Head; Human; In Situ Hybridization; Injury; Instruction; Kinesin; Limb structure; Mammals; Mesoderm; Microtubules; Minus End of the Microtubule; Modeling; Molecular; Motor; Muscle; Muscle Cells; Muscle Fibers; Natural regeneration; Organ; Organism; Pathway interactions; Pattern; Pharmaceutical Preparations; Phenotype; Planarians; Platyhelminths; Pluripotent Stem Cells; Process; Proteins; RNA Interference; Regenerative capacity; Regenerative pathway; Regenerative response; Regulation; Role; Signal Induction; Signal Transduction; Site; Specific qualifier value; Structure; Surgical Injuries; Surgical wound; Tail; Testing; Tissues; Transport Process; WNT Signaling Pathway; Wnt proteins; beta catenin; cell type; experimental study; eye formation; eye regeneration; inhibitor; injured; insight; neuronal cell body; novel; response; response to injury; restoration; small molecule; stem cells; stem-like cell; tissue regeneration; wound

Project Summary Tissue regeneration is the process of renewal, restoration and growth that allows an organism to re-grow limbs and organs after injury. Tissue regeneration abilities are present in diverse organisms, suggesting they may have been ancient, so the mechanisms controlling regeneration have been important to understand. The freshwater planarian flatworm Schmidtea mediterranea regenerates from nearly any surgical injury, including decapitation, and is a model for understanding the mechanistic basis of animal regeneration. After injury, tissue near wound sites signals to nearby adult pluripotent stem cells called neoblasts, which differentiate to replace all cell types of missing tissue. The highly conserved Wnt signaling pathway is essential for controlling regeneration of anterior-versus-posterior tissue identity in planarian regeneration. The earliest event in head versus tail determination is the injury induced expression of the secreted Wnt inhibitor notum selectively at anterior-facing and not posterior-facing wound sites. However, the mechanism directing this symmetry breaking event is unknown. Injury induced notum expression occurs from the soma of body wall muscle cells possessing longitudinally aligned muscle fibers, suggesting a role of muscle cell orientation and polarity in the onset of regeneration. However, the molecular signals and structure regulating this polarity are unknown, but classic small molecule inhibition studies in planaria suggest a possible role for microtubules in this process. The goal of this project is to uncover the mechanisms by which microtubules participate in the polarity of the regeneration response in the following specific aims. Aim 1 utilizes RNAi to test roles for microtubule regulatory factors expressed in muscle to control injury-induced gene expression at the onset of tissue regeneration. Aim 2 uses epistasis analysis with RNAi to uncover the mechanism by which a kinesin microtubule motor protein relates to a Wnt pathway for head and eye regionalization used in regeneration. These experiments will provide insight on the cellular and molecular mechanism of microtubules during injury induced regeneration. My results will provide new insights into understanding the other factors necessary for proper injury signal and help form a better understanding on what regulates polarity.

项目总结