Pseudomonas-Macrophage IL-1β Interactions in Lung Transplant Recipients

肺移植受者中假单胞菌-巨噬细胞 IL-1β 的相互作用

• 批准号: 10606768
• 负责人: Rachel Noel Britton
• 金额: $ 6.95万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10606768
• 关键词: Address; Allografting; Alveolar; Alveolar Macrophages; Bacteria; Bioinformatics; Bronchoalveolar Lavage; CASP1 gene; Categories; Cell Line; Cessation of life; Chronic; Chronic Lung Injury; Clinical; Critical Thinking; Data; Development; Diagnosis; Exposure to; Fellowship; Functional disorder; Future; Human; IL18 gene; IL8 gene; Immune; Immunophenotyping; Impairment; Incidence; Individual; Infection; Inflammasome; Inflammation; Inflammatory; Inflammatory Response; Injury; Innate Immune Response; Interleukin-1 beta; Interleukin-6; Kinetics; Knowledge; Laboratories; Life; Link; Lung; Lung Transplantation; Lung diseases; Macrophage; Measures; Mediating; Mediator; Methods; Modeling; National Research Service Awards; Outcome; Phagocytes; Phenocopy; Phenotype; Play; Population; Preventive; Production; Proteins; Pseudomonas; Pseudomonas aeruginosa; Pulmonary Inflammation; Research; Ribosomal RNA; Risk Factors; Role; Sampling; Scientist; TNF gene; Testing; Transplant Recipients; Transplantation; Upper respiratory tract; bactericide; biobank; career; clinical diagnosis; cytokine; graft failure; host-microbe interactions; immune activation; inhibitor; lung allograft; lung colonization; lung injury; lung microbiome; lung microbiota; microbial; microbiome; microbiome alteration; monocyte; novel; pathogen; pharmacologic; prevent; pulmonary function; research and development; response; sensor; skills; therapeutic target

The objectives of this NRSA individual fellowship are to 1) facilitate the development of research skills necessary for the applicant to become an effective and independent scientist and 2) investigate the mechanisms behind Pseudomonas aeruginosa-induced interleukin-1 beta (IL-1β) production in lung transplantation. Lung transplantation is a life-extending treatment for individuals with end-stage lung diseases; however, long-term outcomes are limited by allograft injury and chronic lung allograft dysfunction (CLAD). While CLAD is defined as a clinical diagnosis with median onset several years after transplantation, the antecedent risk factors may occur months or years prior to diagnosis, supporting a hypothesis that early inflammatory mechanisms could represent important triggers for lung inflammation and injury. Increased bacterial burden, decreased bacterial diversity, and prominence of P. aeruginosa are associated with worsening lung function, graft failure, and CLAD through pathogen-driven inflammatory triggers and impaired innate responses impacting bacterial clearance. The applicant has preliminary data demonstrating that P. aeruginosa upregulates sustained IL-1β production in lung transplant recipient-derived alveolar macrophages compared to other common upper respiratory tract bacteria. Elevated IL-1β has been associated with a decline in lung function and lung inflammation, and chronic lung injury, but few studies have examined the role of pathogen-driven inflammatory triggers on human activation of alveolar macrophage innate immune responses in lung transplantation. This proposal focuses on the mechanisms underlying the interaction between the lung microbiota and its innate sensing by macrophages to address this knowledge gap. We hypothesized that the lung microbiome, particularly those dominated by P. aeruginosa, following transplantation mediates sustained alveolar macrophage proinflammatory responses. To assess the effects of the lung microbiome on macrophages, we used a novel method to isolate lung microbiota from lung transplant recipients for stimulation of macrophages. This proposal uses two approaches to evaluate the impact of P. aeruginosa and Pseudomonas-dominant lung microbiota on immune activation and inflammation: 1) focusing on IL-1β production through activation of the inflammasome using specific canonical sensor proteins and 2) focusing on macrophage immunophenotype. Rigorous immunophenotyping and advanced bioinformatic analysis will be used to elucidate the relationship between P. aeruginosa and inflammasome-activated macrophage IL-1β production in lung transplant recipients. This proposal will add a novel understanding of cellular mechanisms linking microbial lung populations to aberrant innate immune activation, which is critical to developing future therapeutic targets and preventing immune-mediated lung injury. This proposal will provide a rich opportunity for the applicant to establish the laboratory and critical thinking skills necessary for a successful research career.

NRSA个人奖学金的目标是1)促进必要研究技能的发展