Using auxin to understand context-dependent hormone response

使用生长素了解背景依赖性激素反应

• 批准号: 10605909
• 负责人: JOSEPH CAMMARATA
• 金额: $ 1.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2023-03-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10605909
• 关键词: ATAC-seq; Affect; Algorithms; Arabidopsis; Automobile Driving; Auxins; Bayesian Analysis; Binding; Binding Sites; Biological Assay; Biology; Categories; Cells; Chromatin; Complex; DNA; DNA Binding; Darkness; Data; Dependence; Development; Disease; Early identification; Engineering; Environment; Environmental Risk Factor; Event; Faculty; Future; Gene Expression Regulation; Genes; Genetic; Genetic Transcription; Germination; Goals; Growth; Hormones; Hypocotyl; Label; Laboratories; Life; Light; Malignant Neoplasms; Mediator; Modeling; Mouse-ear Cress; Mutagens; Outcome; Output; Pathway Analysis; Pathway interactions; Pattern; Plant Growth Regulators; Plants; Research; Resistance; Seedling; Signal Pathway; Signal Transduction; Stimulus; System; Systems Biology; Testing; Time; Tissue-Specific Gene Expression; Tissues; Training; Transcription Coactivator; Variant; Work; career; causal variant; cell fate specification; cell type; cofactor; falls; fascinate; gene regulatory network; genetic corepressor; genetic resource; mutant; novel; nuclease; plant growth/development; programs; protein complex; response; reverse genetics; tenure track; transcription factor; transcriptome sequencing

Title: Using auxin to understand context-dependent hormone response Project Summary Cells constantly integrate a suite of intrinsic and extrinsic information to coordinate their activities. The suite of stimuli perceived by a cell – the context – can drastically alter the response to a subsequent stimulus. The plant hormone auxin provides a fascinating example of this context-dependent response: auxin regulates diverse phenomena from cell expansion to cell fate specification during all aspects of a plant's life. How the same hormone can elicit such diverse responses depending on the context is unknown. Here I propose work to discover genetic factors required for context-depending auxin response. Seedlings of Arabidopsis thaliana respond oppositely to exogenous auxin when grown in the light versus when grown in darkness. I hypothesize that auxin triggers distinct genetic pathways in light and darkness. Using Arabidopsis as a model, I will test this hypothesis first by performing a mutant screen that will isolate novel factors required for context-dependent and context-independent auxin signaling. I will subsequently assay how the DNA-binding landscape of AUXIN RESPONSE FACTOR (ARF) transcriptional effectors compares in light and dark-grown hypocotyls. If ARF binding varies substantially due to context, I will test whether differences in chromatin accessibility underlies this variation. If ARF DNA-binding is similar across contexts, then other factors in complex with the ARFs are likely responsible for modulating auxin response; these will be investigated using TURBO-ID to identify ARF protein complex components. Finally, I will perform time course RNA-seq followed by Gene Regulatory Network (GRN) analysis to assess how the transcriptional response to auxin changes in the light versus in the darkness. Altogether the mutant screen, ARF DNA-binding profiles, and GRNs will elucidate how context affects the response to a stimulus and identify important factors in context-dependent signaling that can be investigated in the future.

题目：利用生长素了解环境依赖性激素反应