Novel role of mitotic kinesin KIF11 in structural plasticity and memory

有丝分裂驱动蛋白 KIF11 在结构可塑性和记忆中的新作用

• 批准号: 10607093
• 负责人: Jenna Lynne Wingfield
• 金额: $ 6.76万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10607093
• 关键词: Alzheimer' s Disease; Architecture; Behavior; Behavioral Paradigm; Binding; Binding Proteins; Biological Assay; Brain; COX7A2L Protein; Cells; Data; Defect; Dendrites; Dendritic Spines; Development; Disease; Dorsal; Dynein ATPase; Electrophysiology (science); Ensure; Exhibits; Extinction (Psychology); Face; Frequencies; Genes; Glutamates; Growth; Health; Hippocampus (Brain); Human; Image; Intellectual functioning disability; Intracellular Transport; Kinesin; Learning; Learning Disorders; Lymphedema; Mediating; Mediator of activation protein; Memory; Mental Retardation; Microcephaly; Microtubule Proteins; Microtubules; Mitosis; Mitotic; Morphology; Motor; Mus; Mutation; Neurobiology; Neurons; Operative Surgical Procedures; Penetrance; Physiological; Protein Family; Proteins; RNA Interference; Regulation; Role; Synapses; Synaptic Transmission; Synaptic Vesicles; Synaptic plasticity; Synaptophysin; Syndrome; Testing; Training; Vertebral column; Work; autism spectrum disorder; density; experimental study; gain of function; in vivo; insight; knock-down; long term memory; loss of function; member; mental function; nervous system disorder; novel; overexpression; pleiotropism; polarized cell; preservation; protein function; synaptic function; therapeutic development; two photon microscopy

Project Summary Mitotic cells dynamically regulate microtubule (MT) architecture through the use of the MT motors: kinesin and dynein. Intriguingly, many of the canonical mitotic kinesins are expressed in post-mitotic neurons. These kinesins are thought to participate in repositioning MTs in neurons, yet their precise function and how they are regulated in neurons are unknown. Notably, mutations in kinesin-5 result in disorders associated with intellectual disabilities in humans, however, there is a lack of mechanistic understanding of why this occurs. Previously, the Puthanveettil lab revealed that members of the kinesin family of proteins (KIFs) are physiologically regulated in neurons and are required for long-term memory storage. Recent studies from the lab uncovered that KIF11 (Kinesin-5), acts as an inhibitory constraint on excitatory synaptic transmission in hippocampal neurons. RNAi- mediated loss-of-function analysis of KIF11 resulted in increased mini excitatory post-synaptic currents frequencies, increased dendritic branching, and increased synapse density. KIF11 is the only known homotetrameric kinesin, with four, slow motor domains that face outward to bind interpolar MTs during mitosis, and brake against other kinesins to ensure proper spindle formation. It is unknown how KIF11 mediates structural changes in hippocampal neurons, and whether KIF11 has a role in long-term memory storage. My central hypothesis is that KIF11 mediated regulation of MT dynamics is required for the activity-dependent structural changes in dendritic spines and long-term memory. To test this, in Aim1 I will uncover the mechanism behind how KIF11 regulates structural plasticity through examining activity-dependent changes in spine morphology following glutamate uncaging in WT, KIF11 KD, and KIF11 overexpression neurons by two-photon microscopy (2P) imaging while simultaneously visualizing MT dynamics via EB1. In Aim 2 I will test the effects of the loss of function and gain of function of KIF11 in dorsal CA1 neurons in learning and memory behavior assays. Completion of these aims will bring novel insights into the mechanism and regulation of KIF11 in structural plasticity and memory. Moreover, these studies will significantly advance our understanding of mitotic kinesins in post-mitotic neurons.

项目概要 有丝分裂细胞通过使用 MT 马达动态调节微管 (MT) 结构：驱动蛋白和 动力蛋白。有趣的是，许多典型的有丝分裂驱动蛋白在有丝分裂后神经元中表达。这些驱动蛋白 被认为参与神经元中 MT 的重新定位，但它们的精确功能及其调节方式 神经元中的情况未知。值得注意的是，驱动蛋白 5 的突变会导致与智力障碍相关的疾病 然而，在人类中，人们对为什么会发生这种情况缺乏机械性的理解。此前， Puthanveettil 实验室揭示了驱动蛋白家族 (KIF) 的成员在生理学上受到调节 神经元，是长期记忆存储所必需的。实验室最近的研究发现 KIF11 (Kinesin-5)，作为海马神经元兴奋性突触传递的抑制性约束。 RNAi- KIF11 介导的功能丧失分析导致微小兴奋性突触后电流增加 频率、树突分支增加和突触密度增加。 KIF11 是唯一已知的 同四聚体驱动蛋白，具有四个慢速运动结构域，在有丝分裂期间面向外结合极间 MT， 并制动其他驱动蛋白以确保纺锤体的正确形成。目前尚不清楚 KIF11 如何介导结构 海马神经元的变化，以及 KIF11 是否在长期记忆存储中发挥作用。我的中央 假设 KIF11 介导的 MT 动力学调节是活性依赖的结构所必需的 树突棘和长期记忆的变化。为了测试这一点，在 Aim1 中我将揭示背后的机制 KIF11 如何通过检查脊柱形态的活动依赖性变化来调节结构可塑性 通过双光子显微镜观察 WT、KIF11 KD 和 KIF11 过表达神经元中谷氨酸解笼锁后的情况 (2P) 成像，同时通过 EB1 可视化 MT 动态。在目标 2 中，我将测试损失的影响 KIF11 在学习和记忆行为测定中背侧 CA1 神经元的功能和功能获得。 这些目标的完成将为KIF11的结构机制和调控带来新的见解。 可塑性和记忆力。此外，这些研究将显着增进我们对有丝分裂驱动蛋白的理解 在有丝分裂后神经元中。