Conformational Energetics and Heterogeneity to Reveal Gating Mechanisms of TRPV and TRPM Ion Channels

项目总结/摘要 这一促进多样性的行政补充请求将使Teresa M Swanson博士能够接受培训 戈登实验室的博士后学者她将在R35赠款范围内从事TRPM 2项目。 本项目旨在研究钙离子和ADP核糖驱动构象的分子机制， TRPM 2通道中的重排。斯旺森博士将在戈登博士的直接指导下 所需方法：使用稳态和时间分辨荧光的过渡金属离子FRET 测量;使用琥珀密码子抑制引入非天然氨基酸;和膜片钳 荧光测定法这个项目的工作将为斯旺森博士提供良好的培训，以促进她的职业生涯。她将 还参加职业发展机会和计划，以提高生物医学的多样性 如培训计划中所述。

Project Summary/Abstract This request for an administrative supplement to promote diversity will allow Dr. Teresa M Swanson to train as a postdoctoral scholar in the Gordon lab. She will work on the TRPM2 project in the scope of the parent R35 grant. This project addresses the molecular mechanisms governing Ca2+- and ADP Ribose-driven conformational rearrangements in TRPM2 channels. Dr. Swanson will train directly under Dr. Gordon in the experimental methods required: transition metal ion FRET using both steady-state and time-resolved fluorescence measurements; using amber codon suppression to introduce unnatural amino acids; and patch-clamp fluorometry. Work on this project will provide Dr. Swanson with excellent training to advance her career. She will also participate in career development opportunities and programs to enhance diversity of the biomedical workforce, as described in the training plan.