Diversity in a Dish: Pluripotent Stem Cells in Genetic Analysis and Disease Modeling

培养皿中的多样性：遗传分析和疾病建模中的多能干细胞

• 批准号: 10608751
• 负责人: Christopher Lee Baker
• 金额: $ 2万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10608751
• 关键词: Acceleration; Active Learning; Address; Adopted; Attention; Automobile Driving; Award; Awareness; Bioinformatics; Biological; Cell Differentiation process; Cell Line; Cell Therapy; Cell model; Cells; Cellular biology; Collaborations; Communities; Community Developments; Computer Analysis; Creativeness; Data; Development; Discipline; Disease; Disease model; Disparity population; Educational workshop; Engineering; Ensure; Environment; Equity; Ethics; Ethnic Origin; Faculty; Fostering; Funding; Future; Genetic; Genetic Diseases; Genetic Research; Genetic Variation; Genomics; Goals; Grant; Health; Heterogeneity; High-Throughput Nucleotide Sequencing; Human; Human Biology; Human Cell Line; Human Genetics; Hybrids; In Vitro; Interdisciplinary Study; Laboratories; Medical; Medicine; Mentorship; Methodology; Methods; Modeling; Molecular; Mus; Mutant Strains Mice; Nature; Patient Recruitments; Persons; Phenotype; Pluripotent Stem Cells; Population; Population Heterogeneity; Problem Solving; Public Health; Published Comment; Reagent; Recording of previous events; Recruitment Activity; Regenerative Medicine; Reproducibility; Research; Research Personnel; Research Project Grants; Research Project Summaries; Resource Development; Resource Sharing; Resources; Scholarship; Science; Scientist; Statistical Methods; Stem Cell Research; Study models; System; Technical Expertise; Technology; Teratoma; The Jackson Laboratory; Tissues; Training; Travel; Underrepresented Populations; Undifferentiated; United States National Institutes of Health; Variant; Work; biomedical resource; career; career development; design; directed differentiation; embryonic stem cell; functional genomics; functional outcomes; genetic analysis; genetic approach; genome wide association study; human disease; human model; induced pluripotent stem cell; interdisciplinary collaboration; mouse genetics; multidisciplinary; next generation; personalized therapeutic; pluripotency; precision genetics; programs; rational design; research and development; response; screening; skill acquisition; stem cell biology; stem cells; symposium; tool; virtual

PROJECT SUMMARY Research and experimental use of pluripotent stem cells has expanded rapidly, and these cells now provide important research models of human disease and personalized regenerative medicine. However, the majority of research to date that has utilized stem cells has employed genetically-limited murine cells or clonal human cell lines with little attention paid to the impact of cellular genetic diversity on the phenotypic and functional outcomes and its relevance to human diversity. Importantly, recent data from murine and human studies of pluripotent stem cells indicate that genetic variability is the most significant driver of molecular and cellular differences in phenotype, including the ease of establishment of pluripotent cell lines, the rate of spontaneous differentiation, reprogramming differentiated cells to a pluripotent state, and the future use of stem cells as personalized therapeutics. Thus, there is now an unmet need, not rigorously addressed at current conferences, to understand the consequences of genetic variation on stem cell biology and their impact on development. To fill this gap, we propose the unique and timely conference “Diversity in a Dish: Pluripotent Stem Cells in Genetic Analysis and Disease Modeling” to facilitate interdisciplinary interactions and collaborations between experts in mouse and human genetics, cell biology, and pluripotency, and to engage diverse learners who are the next generation of stem cell researchers. We propose an annual, intensive 3-day conference beginning in 2023 at The Jackson Laboratory in Bar Harbor, ME. We will bring together 50 scientists, spanning career stages, from the fields of genetics, cell biology, and pluripotency, as well as provide a virtual attendance option for those that cannot or choose not to attend in person. For Aim 1, we will convene an interdisciplinary symposium organized around cutting-edge research seminars, workshops that focus on technical skill acquisition, and rigor and reproducibility as applied to stem cell research and genomic analyses. In Aim 2, we will promote interactive and inclusive activities, to promote networking, mentorship, learner involvement, collaborative research, and career development. These activities include trainee presentations and short talks, and evening discussions focused on research projects, rigor and ethics, and grantsmanship. These objectives are facilitated by the residential nature of the JAX Highseas Conference Center. In Aim 3, we will promote diversity in both the recruitment of participants and in the tools being generated to study models of human disease. Diverse attendees will be actively recruited, scholarships will be provided to enable in-person attendance, and an inclusive virtual option will enable participation by those that are unable to travel. Diversity will also be emphasized in the context of best practices for research, including the need for studies of genetically diverse panels of stem cells, and fair acquisition, representation, and distribution of cell lines from wider representation of backgrounds to ensure the benefits discovered from these biomedical resources are shared equitably.

项目摘要 多能干细胞的研究和实验应用已经迅速扩大，这些细胞现在提供了 人类疾病和个性化再生医学的重要研究模型。但大多数 迄今为止利用干细胞的研究已经采用了遗传有限的鼠细胞或克隆的人细胞 很少关注细胞遗传多样性对表型和功能结果的影响 以及它与人类多样性的相关性。重要的是，最近来自小鼠和人类多能干细胞研究的数据 细胞表明，遗传变异性是分子和细胞差异的最重要驱动因素， 表型，包括建立多能细胞系的容易性，自发分化的速率， 将分化的细胞重新编程为多能状态，以及将来将干细胞用作个性化的细胞。 治疗学因此，现在有一个未得到满足的需要，在目前的会议上没有得到严格处理， 遗传变异对干细胞生物学的后果及其对发育的影响。为了填补这一空白，我们 提出独特和及时的会议“多样性在一个菜：多能干细胞在遗传分析和 疾病建模”，以促进跨学科的互动和合作之间的专家在小鼠和 人类遗传学，细胞生物学和多能性，并吸引不同的学习者谁是下一代的 干细胞研究者我们建议从2023年开始在杰克逊酒店举行为期3天的年度密集会议 我们将汇集50名科学家，跨越职业阶段，来自以下领域： 遗传学，细胞生物学和多能性，以及为那些不能或 选择不亲自参加。为了实现目标1，我们将召开一次跨学科研讨会， 尖端研究研讨会，专注于技术技能获取的研讨会，以及严谨性和可重复性 应用于干细胞研究和基因组分析。在目标2中，我们将促进互动和包容 活动，以促进网络，导师，学习者参与，合作研究和职业生涯 发展这些活动包括学员介绍和简短的会谈，以及晚上的讨论， 关于研究项目，严谨性和道德，以及保证金。这些目标是由住宅促进 JAX Highseas会议中心在目标3中，我们将在招聘 参与者和正在生成的研究人类疾病模型的工具。不同的与会者将 积极招募，将提供奖学金，使亲自出席，并提供一个包容性的虚拟选项 将使那些无法旅行的人能够参加。多样性也将在以下方面得到强调： 研究的最佳做法，包括需要研究遗传多样性的干细胞小组， 从更广泛的背景代表性中获取、代表和分配细胞系，以确保 从这些生物医学资源中发现的利益得到公平分享。