Experimental Integration, Design and Analysis Core

实验集成、设计和分析核心

• 批准号: 10608063
• 负责人: Janet L Stein
• 金额: $ 44.06万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10608063
• 关键词: Acceleration; Achievement; Antibodies; Area; Bioinformatics; Biological Models; Biometry; Biopsy Specimen; Cancer Biology; Cell Line; Cell model; Cells; Clinical; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Complement; Computer Analysis; Data; Data Analyses; Data Set; Data Storage and Retrieval; Dimensions; Ensure; Epigenetic Process; Experimental Designs; Faculty; Future; Generations; Genome; Goals; Human Resources; Individual; Informatics; Investigation; Laboratories; Libraries; Methods; Molecular; Organoids; Output; Pathology; Patients; Positioning Attribute; Procedures; Property; Protocols documentation; Quality Control; Reagent; Reproducibility; Research; Research Personnel; Resources; Sampling; Services; Source; Standardization; Technology; Testing; Tissue Microarray; Tissues; Translating; Tumor Tissue; Validation; Work; biobank; clinical application; clinical care; data integration; data sharing; data standards; design; epigenetic regulation; genome editing; genomic data; high throughput analysis; imaging study; innovation; insight; knowledge base; malignant breast neoplasm; next generation sequencing; novel; programs; public database; reagent standardization; synergism; translational applications; treatment response

Summary: Experimental Integration, Design and Analysis Core The Experimental Integration, Design, and Analysis (EIDA) Core is an essential component of the program project that is uniquely designed to implement novel cellular, molecular and informatic strategies, and to synergize collaboration and integration of Program investigation to drive scientific discoveries. The Program is thematically centered on novel mechanisms of epigenetic regulation that are relevant to breast cancer. The EIDA Core is designed to capture the momentum of the Program, as well as to provide cohesive experimental and analytical design that will ensure rigor and reproducibility. The EIDA Core will centralize experimental design, bioinformatics support for data sharing and integration, and biostatistical analyses for patient-based, cell- and organoid-derived findings. The Core will ensure that every project is using standardized reagents, protocols and bioinformatics methods, such that data comparisons among projects can be made. Each project uses cutting- edge omics-based approaches; the application of these technologies necessitates significant expertise and implementation of best practices for scientific rigor and transparency, which will be provided by the EIDA Core. Support by the Core is essential for discoveries that will be generated by each of the projects. The goal of the EIDA core is to maximize integration of experimental effort and output. This innovative multi- dimensional core will accelerate research progress of the Program. The Aims of the EIDA Core are to provide: 1) Generation and authentication of biological models and reagents. Stringent quality control of reagents and uniform experimental protocols are essential for data comparison across all projects. 2) Analyses and integration of next generation sequencing data will assure that multiple types of genomic data can be compared for biologically relevant interpretation. Centralization of bioinformatics analysis in the Core positions the Program bioinformatician to perform analyses with the overall Program strategy and goals in mind, so that data generated by each Project can be used to inform and integrate results with the other projects. 3) Rigor and reproducibility of experimental strategies through statistical validation of the findings will enhance and strengthen discoveries that can be translated to clinical care in future studies. Impact: The EIDA core will enable integration of datasets by connecting the shared and distinct properties of epigenetic control and genome organization with breast cancer onset, progression and treatment response.

总结：实验集成，设计和分析核心 实验集成，设计和分析（EIDA）核心是该计划的重要组成部分 该项目是独特的设计，以实现新的细胞，分子和信息战略，并 协同合作和整合项目调查，推动科学发现。该计划是 主题集中于与乳腺癌相关的表观遗传调控的新机制。EIDA 核心旨在捕捉该计划的势头，以及提供有凝聚力的实验和 分析设计，以确保严谨性和再现性。EIDA核心将集中实验设计， 生物信息学支持数据共享和整合，以及基于患者、细胞和 类器官衍生的发现核心将确保每个项目都使用标准化的试剂、方案和 生物信息学方法，这样就可以在项目之间进行数据比较。每个项目都使用切割- 基于边缘组学的方法;这些技术的应用需要大量的专业知识， 实施科学严谨性和透明度的最佳实践，这将由EIDA核心提供。 核心的支持对于每个项目产生的发现至关重要。 EIDA核心的目标是最大限度地整合实验工作和输出。这种创新的多- 三维核心将加速该计划的研究进展。EIDA核心的目标是： 1)生物模型和试剂的生成和验证。严格的试剂质量控制， 统一的实验方案对于所有项目的数据比较至关重要。2)分析和整合 下一代测序数据将确保可以比较多种类型的基因组数据， 生物学相关的解释。将生物信息学分析集中在核心位置， 生物信息学家执行分析与整体计划战略和目标铭记，使数据生成 每个项目都可以用来通知和整合其他项目的结果。3)严格性和重现性 通过对研究结果进行统计验证的实验策略将提高和加强发现 可以在未来的研究中转化为临床护理。 影响：EIDA核心将通过连接共享的和不同的属性， 表观遗传控制和基因组组织与乳腺癌发病、进展和治疗反应。