Multiplexed electronic counting of scarce protein targets using nucleic acid nanoparticles
使用核酸纳米粒子对稀有蛋白质靶标进行多重电子计数
基本信息
- 批准号:10611370
- 负责人:
- 金额:$ 18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:AddressAffinityAntibodiesBindingBinding ProteinsBiologicalBiological AssayBiological MarkersCancer DiagnosticsCharacteristicsClinicalCollaborationsComplexComplex MixturesDNA-Binding ProteinsDetectionDevelopmentDevicesDimensionsDiseaseEarly DiagnosisElasticityElectronicsElementsEmerging Communicable DiseasesEnzyme-Linked Immunosorbent AssayEventFoundationsIncubatedIndividualLaboratoriesLibrariesLifeLiquid substanceLiteratureMeasurementMeasuresMembraneMicroscopicModelingNanostructuresNanotechnologyNucleic AcidsPathogenicityPatient CareProteinsRNA-Binding ProteinsResearchResearch PersonnelResearch ProposalsSamplingShapesSignal TransductionSpecificityStructureTechniquesTechnologyTimeTissuesUniversitiesantibody conjugateaptamerclinical applicationcombinatorialcostcost effectivedesigndimerelectric fieldexperimental studyhigh rewardhigh riskimprovedindividual patientinnovationinstrumentminimally invasivemultiplex detectionnanonanoparticlenanoporenovel strategiesparticlepersonalized medicineportabilityprenatalprotein biomarkersrapid diagnosisresidenceresponseself assemblysensor technologysimulationsingle moleculesolid statesynergismtechnology developmentvirtual
项目摘要
PROJECT SUMMARY
This interdisciplinary project synergizes the expertise of three research groups for a proof-of-principle
demonstration of a novel approach for accurate and sensitive detection of scarce protein biomarkers. The key
innovative element of the approach is the use of wireframe-like nucleic acid nanoparticles (NANPs) to bind
protein targets with high affinity either directly or by means of auxiliary antibody proteins. Binding of the protein
targets is detected by first incubating the sample with a cocktail of NANPs and then examining them using a
nanopore in a solid-state membrane. Protein detection relies on measurement of the ionic current flowing through
the nanopore: the ionic current and particle dwell time decreases by a characteristic amount when a NANP of a
certain type enters the nanopore. Importantly, by matching the physical dimensions of the NANP to the physical
dimension of the nanopore, we expect to dramatically increase the residence type of the nucleic acid
nanoparticles within the nanopore and thereby achieve ultra-sensitive (sub-picomolar range) detection of the
protein-bound biomarkers. By designing our NANP probes to produce distinct ionic signatures when bound to
their protein targets, we will achieve multiplex detection of several protein species using the same nanopore as
well as combinatorial detection of biomarkers by assembling the NANP probes and protein biomarkers into a
sandwich like structures. The project will be carried out by the Afonin group at UNC Charlotte that will design
NANPs, the Wanunu group at Northeastern University that will perform the nanopore detection experiments, and
the Aksimentiev group that will use an arsenal of modeling techniques to optimize and improve the detection
strategy. Our ultra-sensitive, portable, rapid, and potentially low-cost technology for quantification of protein
levels is expected to find broad use for the analysis of biological samples, eventually offering sensitive, reliable,
and minimally invasive identification of disease-indicative biomarkers that could be important innovations for
early-stage diagnostics of cancer and other diseases.
项目总结
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kirill A Afonin其他文献
Kirill A Afonin的其他文献
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{{ truncateString('Kirill A Afonin', 18)}}的其他基金
Multiplexed electronic counting of scarce protein targets using nucleic acid nanoparticles
使用核酸纳米粒子对稀有蛋白质靶标进行多重电子计数
- 批准号:
10353490 - 财政年份:2022
- 资助金额:
$ 18万 - 项目类别:
SMART NANPs: new molecular platform for communication with human immune system and modulation of therapeutic responses
SMART NANP:与人体免疫系统通讯和调节治疗反应的新分子平台
- 批准号:
10331771 - 财政年份:2021
- 资助金额:
$ 18万 - 项目类别:
SMART NANPs: new molecular platform for communication with human immune system and modulation of therapeutic responses
SMART NANP:与人体免疫系统通讯和调节治疗反应的新分子平台
- 批准号:
10557793 - 财政年份:2021
- 资助金额:
$ 18万 - 项目类别:
Administrative Supplement to SMART NANPs: new molecular platform for communication with human immune system and modulation of therapeutic responses
SMART NANP 的行政补充:用于与人体免疫系统沟通和调节治疗反应的新分子平台
- 批准号:
10798083 - 财政年份:2021
- 资助金额:
$ 18万 - 项目类别:
Characterization of various multifunctional nucleic acid nanoparticles and understanding their immunotoxicity
各种多功能核酸纳米粒子的表征并了解其免疫毒性
- 批准号:
10013239 - 财政年份:2017
- 资助金额:
$ 18万 - 项目类别:
Characterization of various multifunctional nucleic acid nanoparticles and understanding their immunotoxicity
各种多功能核酸纳米粒子的表征并了解其免疫毒性
- 批准号:
9384048 - 财政年份:2017
- 资助金额:
$ 18万 - 项目类别:
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