Project 2: Leveraging Metagenomics of the Microbiome to predict colonization/infection by antimicrobial-resistant pathogens
项目 2:利用微生物组的宏基因组学来预测耐药病原体的定植/感染
基本信息
- 批准号:10614694
- 负责人:
- 金额:$ 54.31万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-01 至 2026-07-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAnimal ModelAntimicrobial ResistanceAreaCharacteristicsClassificationClinicalClinical DataClinical ResearchClostridium difficileCohort StudiesCommunicable DiseasesCritical IllnessDataData AnalysesDevelopmentElementsExtended-spectrum β-lactamaseFecesFunctional disorderGenomicsGoalsHealthHematopoietic Stem Cell TransplantationHospitalsHumanHuman MicrobiomeImmunocompromised HostInfectionIntensive Care UnitsIntestinesKnowledgeLength of StayLongitudinal StudiesMediatingMedical centerMetagenomicsMusNatureOrganismPathogenesisPatientsPhysiciansPredispositionPreventiveProtocols documentationPublic HealthPublishingResearchResistanceRibosomal RNARiskSamplingScientistScourgeSignal TransductionSiteSystemSystems BiologyTestingTexasTherapeuticTransplant RecipientsVancomycin resistant enterococcusWorkantimicrobialantimicrobial resistant infectionantimicrobial resistant pathogenbasecarbapenem-resistant Enterobacteriaceaecohortcolonization resistancecommensal microbesdeep sequencingdrug resistant pathogengulf coastgut colonizationgut microbiomegut microbiotahigh riskindexinginsightmicrobiomemicrobiome analysismicrobiome compositionmicrobiotamicrobiota profilesmulti-drug resistant pathogennew technologynovelpathogenpatient populationpatient subsetsprogramsrisk stratificationstool samplesynergismtool
项目摘要
ABSTRACT
Leveraging Metagenomics of the Microbiome to Predict Colonization/Infection by Antimicrobial-
Resistant Pathogens (Project #2)
Antimicrobial resistance is a growing, global threat to public health. Vancomycin-resistant enterococci (VRE),
extended spectrum β-lactamase producing/carbapenem-resistant Enterobacteriaceae (ESBL-E/CRE) and
Clostridiodes difficile are key antimicrobial resistant (AMR) pathogens that share the intestine as the initial site
of colonization. Therefore, colonization resistance provided by the commensal microbiota of the intestines is a
critical aspect of the pathophysiology of these organisms. The completion of the initial stages of the Human
Microbiome Project has provided new understanding of how the microbiome impacts infections and has
generated novel tools for further advances in this critical area of human health. Additionally, unbiased
approaches to bacterial identification have resulted in increasing appreciation that VRE, ESBL-E/CRE, and C.
difficile often co-colonize patients suggesting that these organisms are interacting with each other in addition to
the commensal microflora. The long term goals of this project, in synergy with other portions of this P01 proposal,
are to dissect the mechanisms underlying how interactions among the commensal microflora, the host, and VRE,
ESBL-E/CRE, and C. difficile impact intestinal colonization and subsequent infection by these AMR pathogens.
Although it is well known that microbiome disruption by antimicrobials is a key initial step in colonization by these
pathogens, we seek to address the key knowledge gap of why only a subset of patients receiving antimicrobials
become colonized and eventually infected by these organisms. To this end, we propose performing longitudinal
studies of intensive care unit and hematopoietic stem cell transplant patients at two distinct hospitals in the Texas
Medical Center. Patients will be classified depending on both initial and longitudinal colonization status, and
these classifications will be correlated with metagenomics based microbiome analyses of serial stool samples.
In concert with computational biologists, the metagenomics data will be mined for particularly species or
combinations of species that are either protective against or positively associated with colonization and infection,
including co-colonization. Additionally, we will test whether samples from the clinical cohort can protect mice
from AMR pathogen challenge to validate associations observed clinically. Finally, we will also use animal
models to test how pre-existing colonization with a particular organism under study impacts subsequent
colonization by a distinct AMR pathogen. By synergizing with microbiota experts, computational biologists, and
physician-scientists from the highly integrated Gulf Coast Consortium on Antimicrobial Resistance, this proposal
seeks to sharpen understanding of how critical AMR pathogens colonize and infect humans in order to provide
a critical platform for novel preventive or therapeutic approaches to mitigate the AMR scourge.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
SAMUEL A SHELBURNE其他文献
SAMUEL A SHELBURNE的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('SAMUEL A SHELBURNE', 18)}}的其他基金
Role of β-lactamase encoding gene amplification in the development of non-carbapenemase producing, carbapenem-resistant Enterobacteriaceae
β-内酰胺酶编码基因扩增在产生非碳青霉烯酶、耐碳青霉烯肠杆菌科细菌中的作用
- 批准号:
10373951 - 财政年份:2021
- 资助金额:
$ 54.31万 - 项目类别:
Impact of regulatory cross-talk on the pathophysiology of emergent acapsular group A streptococcus
监管串扰对新出现的无荚膜 A 族链球菌病理生理学的影响
- 批准号:
10301505 - 财政年份:2021
- 资助金额:
$ 54.31万 - 项目类别:
Impact of regulatory cross-talk on the pathophysiology of emergent acapsular group A streptococcus
监管串扰对新出现的无荚膜 A 族链球菌病理生理学的影响
- 批准号:
10449272 - 财政年份:2021
- 资助金额:
$ 54.31万 - 项目类别:
Project 2: Leveraging Metagenomics of the Microbiome to predict colonization/infection by antimicrobial-resistant pathogens
项目 2:利用微生物组的宏基因组学来预测耐药病原体的定植/感染
- 批准号:
10226288 - 财政年份:2020
- 资助金额:
$ 54.31万 - 项目类别:
Project 2: Leveraging Metagenomics of the Microbiome to predict colonization/infection by antimicrobial-resistant pathogens
项目 2:利用微生物组的宏基因组学来预测耐药病原体的定植/感染
- 批准号:
10024960 - 财政年份:2020
- 资助金额:
$ 54.31万 - 项目类别:
Contribution of catabolite control protein A to group A streptococcal virulence
分解代谢物控制蛋白 A 对 A 组链球菌毒力的贡献
- 批准号:
8300803 - 财政年份:2011
- 资助金额:
$ 54.31万 - 项目类别:
Contribution of catabolite control protein A to group A streptococcal virulence
分解代谢物控制蛋白 A 对 A 组链球菌毒力的贡献
- 批准号:
8107818 - 财政年份:2011
- 资助金额:
$ 54.31万 - 项目类别:
Contribution of catabolite control protein A to group A streptococcal virulence
分解代谢物控制蛋白 A 对 A 组链球菌毒力的贡献
- 批准号:
8479310 - 财政年份:2011
- 资助金额:
$ 54.31万 - 项目类别:
Contribution of catabolite control protein A to group A streptococcal virulence
分解代谢物控制蛋白 A 对 A 组链球菌毒力的贡献
- 批准号:
8692634 - 财政年份:2011
- 资助金额:
$ 54.31万 - 项目类别:
Contribution of catabolite control protein A to group A streptococcal virulence
分解代谢物控制蛋白 A 对 A 组链球菌毒力的贡献
- 批准号:
8871663 - 财政年份:2011
- 资助金额:
$ 54.31万 - 项目类别:
相似海外基金
Quantification of Neurovasculature Changes in a Post-Hemorrhagic Stroke Animal-Model
出血性中风后动物模型中神经血管变化的量化
- 批准号:
495434 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Bioactive Injectable Cell Scaffold for Meniscus Injury Repair in a Large Animal Model
用于大型动物模型半月板损伤修复的生物活性可注射细胞支架
- 批准号:
10586596 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
A Comparison of Treatment Strategies for Recovery of Swallow and Swallow-Respiratory Coupling Following a Prolonged Liquid Diet in a Young Animal Model
幼年动物模型中长期流质饮食后吞咽恢复和吞咽呼吸耦合治疗策略的比较
- 批准号:
10590479 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Small animal model for evaluating the impacts of cleft lip repairing scar on craniofacial growth and development
评价唇裂修复疤痕对颅面生长发育影响的小动物模型
- 批准号:
10642519 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Diurnal grass rats as a novel animal model of seasonal affective disorder
昼夜草鼠作为季节性情感障碍的新型动物模型
- 批准号:
23K06011 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Longitudinal Ocular Changes in Naturally Occurring Glaucoma Animal Model
自然发生的青光眼动物模型的纵向眼部变化
- 批准号:
10682117 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
A whole animal model for investigation of ingested nanoplastic mixtures and effects on genomic integrity and health
用于研究摄入的纳米塑料混合物及其对基因组完整性和健康影响的整体动物模型
- 批准号:
10708517 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
A Novel Large Animal Model for Studying the Developmental Potential and Function of LGR5 Stem Cells in Vivo and in Vitro
用于研究 LGR5 干细胞体内外发育潜力和功能的新型大型动物模型
- 批准号:
10575566 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Elucidating the pathogenesis of a novel animal model mimicking chronic entrapment neuropathy
阐明模拟慢性卡压性神经病的新型动物模型的发病机制
- 批准号:
23K15696 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
The effect of anti-oxidant on swallowing function in an animal model of dysphagia
抗氧化剂对吞咽困难动物模型吞咽功能的影响
- 批准号:
23K15867 - 财政年份:2023
- 资助金额:
$ 54.31万 - 项目类别:
Grant-in-Aid for Early-Career Scientists