Regulation and Physiological Roles of Translational Fidelity

翻译保真度的调节和生理作用

• 批准号: 10617051
• 负责人: JIQIANG LING
• 金额: $ 6.48万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10617051
• 关键词: Affect; African American population; Antibiotics; Bacteria; Bacteria sigma factor KatF protein; Biochemical; Biochemistry; Biomedical Research; Candidate Disease Gene; Cell Culture Techniques; Cells; Cellular biology; Communicable Diseases; Critical Pathways; Development; Ensure; Evolution; Experimental Designs; Five-Year Plans; Fluorescence; Future; Genes; Genetic; Goals; Hospitalization; Impairment; Infection; International; Knock-out; Knowledge; Libraries; Mass Spectrum Analysis; Microbiology; Minority Groups; Modeling; Molecular; Molecular Biology; Mutation; Nutrient; Osmolalities; Outcome; Pathway interactions; Physiological; Population; Protein Biosynthesis; Quality Control; Reading; Regulation; Reporter; Research; Resolution; Ribosomes; Role; Salmonella; Salmonella enterica; Scientist; Stress; Students; System; Systems Biology; Temperature; Terminator Codon; Testing; Thinking; Training; Translations; United States; Western Blotting; Work; Writing; bacterial genetics; biological adaptation to stress; career development; career networking; cell motility; cost effective; doctoral student; empowered; environmental stressor; global health; high throughput screening; interest; lambda Spi-1; macrophage; meetings; microbial; new technology; novel; parent grant; pathogen; response; single cell analysis; skills; student mentoring; therapy development; transmission process; underrepresented minority student

PROJECT SUMMARY The goal of this project is to enhance the diversity of the biomedical research workforce by training a Ph.D. student from an underrepresented minority group (African Americans) and facilitate her development towards an independent scientist in the field of microbiology and pathogen-host interactions. The student will receive rigorous training to study pathogen-host interactions, protein synthesis, bacterial genetics, and microbial stress responses. Translational fidelity is critically maintained throughout evolution to ensure accurate protein synthesis. However, the role of translational fidelity in pathogen-host interactions remains largely unknown. The proposed training aims at studying how translational fidelity affects Salmonella-macrophage interactions. During the training, the student will (a) develop important research skills covering bacterial genetics, single-cell analyses, cell culture, molecular biology, and biochemistry, (b) enhance her skills of critical reading, thinking, presentation, and writing, and (c) strengthen her ability of proper experimental design and development of hypothesis-driven research. In addition, the student will have the opportunities to further career development by engaging in presenting research at local/national/international meetings, mentoring students, and taking professional training courses. The outcomes of the proposed training will help elucidate the role of translational fidelity in pathogen-host interactions using Salmonella as a model, further the student’s development as an independent microbiologist, expand the student’s professional network, and promote diversity in the workforce of microbiology and infectious diseases.

项目概要 该项目的目标是通过以下方式增强生物医学研究人员的多样性 培养博士学位来自代表性不足的少数群体的学生 （非裔美国人）并促进她成为该领域的独立科学家 微生物学和病原体-宿主相互作用。学生将接受严格的训练 研究病原体-宿主相互作用、蛋白质合成、细菌遗传学和微生物应激 回应。在整个进化过程中严格保持翻译保真度，以确保准确 蛋白质合成。然而，翻译保真度在病原体-宿主相互作用中的作用 仍然很大程度上未知。拟议的培训旨在研究翻译保真度如何 影响沙门氏菌-巨噬细胞的相互作用。在培训期间，学生将 (a) 发展 重要的研究技能，涵盖细菌遗传学、单细胞分析、细胞培养、分子生物学 生物学和生物化学，(b) 提高​​她的批判性阅读、思考、表达和表达的技能 写作，以及（c）加强她正确的实验设计和开发的能力 假设驱动的研究。此外，学生将有机会进一步发展职业生涯 通过在地方/国家/国际会议上展示研究成果来促进发展， 指导学生并参加专业培训课程。拟议的结果 培训将有助于阐明翻译保真度在病原体-宿主相互作用中的作用 沙门氏菌作为模型，进一步促进学生作为独立微生物学家的发展， 扩大学生的专业网络，促进劳动力的多样性 微生物学和传染病。