Regulation and Physiological Roles of Translational Fidelity

项目总结 这个项目的目标是通过以下方式提高生物医学研究人员的多样性 培养来自少数族裔群体的博士生 (非裔美国人)，并促进她向该领域的独立科学家发展 微生物学和病原体与宿主的相互作用。学生将接受严格的培训，以 研究病原体-宿主相互作用、蛋白质合成、细菌遗传学和微生物应激 回应。在整个进化过程中严格保持翻译保真度，以确保准确 蛋白质合成。然而，翻译保真度在病原体-宿主相互作用中的作用 在很大程度上仍不为人所知。拟议的培训旨在研究翻译忠诚度如何 影响沙门氏菌与巨噬细胞的相互作用。在培训期间，学生将(A)发展 重要的研究技能，包括细菌遗传学、单细胞分析、细胞培养、分子 生物学和生物化学，(B)提高她的批判性阅读、思考、陈述和 写作，以及(C)增强她正确设计和开发实验的能力 假设驱动的研究。此外，学生将有机会进一步发展事业 通过在地方/国家/国际会议上提出研究报告来促进发展， 辅导学生，参加专业培训课程。建议的结果： 培训将有助于阐明翻译保真度在病原体-宿主相互作用中的作用 沙门氏菌作为模型，促进学生作为独立微生物学家的发展， 扩大学生的职业关系网，促进劳动力的多样性 微生物学和传染病。

PROJECT SUMMARY The goal of this project is to enhance the diversity of the biomedical research workforce by training a Ph.D. student from an underrepresented minority group (African Americans) and facilitate her development towards an independent scientist in the field of microbiology and pathogen-host interactions. The student will receive rigorous training to study pathogen-host interactions, protein synthesis, bacterial genetics, and microbial stress responses. Translational fidelity is critically maintained throughout evolution to ensure accurate protein synthesis. However, the role of translational fidelity in pathogen-host interactions remains largely unknown. The proposed training aims at studying how translational fidelity affects Salmonella-macrophage interactions. During the training, the student will (a) develop important research skills covering bacterial genetics, single-cell analyses, cell culture, molecular biology, and biochemistry, (b) enhance her skills of critical reading, thinking, presentation, and writing, and (c) strengthen her ability of proper experimental design and development of hypothesis-driven research. In addition, the student will have the opportunities to further career development by engaging in presenting research at local/national/international meetings, mentoring students, and taking professional training courses. The outcomes of the proposed training will help elucidate the role of translational fidelity in pathogen-host interactions using Salmonella as a model, further the student’s development as an independent microbiologist, expand the student’s professional network, and promote diversity in the workforce of microbiology and infectious diseases.