Role of Cerebrovascular cells in tau processing following traumatic brain injury

脑血管细胞在脑外伤后 tau 蛋白加工中的作用

• 批准号: 10614367
• 负责人: Corbin Bachmeier
• 金额: --
• 依托单位: BAY PINES VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10614367
• 关键词: Affect; Alzheimer' s Disease; Animals; Biological; Biological Process; Blood Vessels; Brain; Brain Injuries; Cell Density; Cells; Cerebrovascular system; Chronic; Clinical; Craniocerebral Trauma; Cytoplasmic Protein; Disease; Disease Progression; Drainage procedure; Environment; Evolution; Extracellular Space; Hippocampus; Human; Injury; Investigation; Liquid substance; Mediator; Metabolism; Microglia; Modeling; Movement; Mus; Nerve Degeneration; Neurodegenerative Disorders; Neurofibrillary Tangles; Neuroglia; Neurons; Outcome; Pathogenesis; Pathologic; Pathology; Pathway interactions; Pericytes; Phagocytes; Play; Prevalence; Process; Public Health; Reporting; Risk; Role; Smooth Muscle Myocytes; Specimen; Tauopathies; Testing; Transgenic Organisms; Trauma; Traumatic Brain Injury; Veterans; Wild Type Mouse; Work; brain cell; cerebrovascular; chronic traumatic encephalopathy; combat; extracellular; glymphatic system; hyperphosphorylated tau; improved; mild traumatic brain injury; military veteran; monomer; mouse model; neurodegenerative phenotype; novel strategies; novel therapeutic intervention; prevent; solute; tau Proteins; tau aggregation; tau-1; uptake; vascular contributions; welfare; wound

One of the prominent pathological features of traumatic brain injury (TBI) is the accumulation of hyperphosphorylated and aggregated tau species in the brain. Tau is a cytoplasmic protein which is believed to be restricted to the intracellular compartment of neurons. However, several recent studies have investigated the existence and role for tau in the extracellular compartments of the brain, and have indicated that increased levels of monomeric tau in the extracellular environment play a major role in the pathogenesis of neurodegenerative tauopathies. In fact, it has been reported that extracellular tau levels in the brain correlate with clinical outcome in TBI. Despite the prevalence and potential importance of extracellular tau in neurodegenerative disease, there is little understanding of how extracellular tau is processed and eliminated from the brain. Our studies demonstrate that brain vascular mural cells (pericytes and smooth muscle cells) are involved in the processing and elimination of extracellular tau. Consistent with other neurodegenerative disorders, we observed a progressive decline in cerebrovascular mural cell expression following repetitive mild TBI (r-mTBI) in mice. Moreover, isolated brain vessels from r-mTBI animals were less able to internalize and process tau than non-injured animals. To our knowledge, these are the first studies to observe perturbations in mural cell expression and tau processing in the context of brain trauma. Importantly, alongside the mural cell disruptions in our mouse r-mTBI model, we observed an accumulation of total tau, phosphorylated tau, and oligomeric tau species in the brain. Additionally, we showed that tau elimination from the brain is reduced in transgenic mural-cell depleted animals compared to wild-type mice. We propose that brain vascular mural cells serve as a pathway for processing and eliminating tau from extracellular brain fluids and that disruption of these cells in TBI leads to tau pathology and neurodegeneration. The objective of this project is to determine the contribution of brain vascular mural cells to the tau pathology observed in TBI. The specific aims of this proposal are, 1) examine mural cell status and tau pathology in mural-cell depleted animals and human TBI brains, 2) evaluate the role of mural cells in tau degradation and elimination and the impact of r-mTBI on these processes, and 3) determine the role of mural cells in paravascular (glymphatic) tau clearance and perivascular tau drainage from the brain following r-mTBI. To determine mural cell status following TBI, tau uptake and various mural cell markers will be examined in isolated cerebrovasculature from mural-cell depleted animals and human TBI brain specimens. To understand the influence of mural cells on tau disposition, tau degradation will be evaluated in isolated brain vasculature from r-mTBI animals and tau elimination from the brain will be examined in mural-cell depleted animals and r-mTBI mice. Lastly, to understand the impact of mural cells on extracellular tau movement in brain fluids following r-TBI, we will examine both paravascular (glymphatic) tau clearance and perivascular tau drainage from the brain in mural-cell depleted animals and r- mTBI mice. The proposed studies will advance our understanding of tau processing and elimination from extracellular brain fluids and determine the contribution of brain vascular mural cells to tau pathology and the neurodegenerative phenotype following TBI.

外伤性脑损伤（TBI）的一个显著病理特征是脑内脂质积累

项目成果

Influence of traumatic brain injury on extracellular tau elimination at the blood-brain barrier.

• DOI: 10.1186/s12987-021-00283-y
• 发表时间: 2021-10-26
• 期刊: Fluids and barriers of the CNS
• 影响因子: 7.3
• 作者: Eisenbaum M;Pearson A;Gratkowski A;Mouzon B;Mullan M;Crawford F;Ojo J;Bachmeier C
• 通讯作者: Bachmeier C

Mural cell dysfunction leads to altered cerebrovascular tau uptake following repetitive head trauma.

• DOI: 10.1016/j.nbd.2020.105237
• 发表时间: 2021-03
• 期刊: Neurobiology of disease
• 影响因子: 6.1
• 作者: Ojo J;Eisenbaum M;Shackleton B;Lynch C;Joshi U;Saltiel N;Pearson A;Ringland C;Paris D;Mouzon B;Mullan M;Crawford F;Bachmeier C
• 通讯作者: Bachmeier C