HIPC U19 Adaptive Immunophenotyping Core

HIPC U19 适应性免疫表型核心

• 批准号: 10598125
• 负责人: KAREN M CEROSALETTI
• 金额: $ 29.55万
• 依托单位: BENAROYA RESEARCH INST AT VIRGINIA MASON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-29 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10598125
• 关键词: Acute; Allergens; Antibodies; Antigens; B-Lymphocytes; Bar Codes; Biological Assay; CD8-Positive T-Lymphocytes; CD8B1 gene; Cells; Clinical; Collaborations; Data; Data Analyses; Eragrostis; Flow Cytometry; Immune; Immune response; Immunology; Immunophenotyping; Innate Immune Response; Laboratories; Lymphoid Cell; Methods; Myeloid Cells; Natural Killer Cells; Nose; Pathogenesis; Phenotype; Play; Population; Procedures; Protocols documentation; Regulatory T-Lymphocyte; Research Project Grants; Rheumatoid Arthritis; Role; Sampling; Side; Sputum; Standardization; System; T cell response; T-Lymphocyte; Viral Respiratory Tract Infection; Vulnerable Populations; Work; adaptive immune response; antigen-specific T cells; autoreactive T cell; data management; effector T cell; experimental study; high dimensionality; monocyte; novel; peripheral blood; respiratory infection virus; respiratory virus; response; γδ T cells

SUMMARY/ABSTRACT The Adaptive Phenotyping Core will provide advanced laboratory approaches to examine global and antigen- specific immune responses on samples generated for the HIPC Center entitled “Systems immunology profiling of respiratory viral infections in vulnerable populations”. This core will serve both Research Projects, receiving samples from the Clinical Core and sharing high quality cellular profiles with the Data Management and Analysis Core. The Adaptive Phenotyping Core has two Specific Aims. In Specific Aim 1, the Adaptive Phenotyping Core will use spectral flow cytometry to generate high-dimensional profiling data on myeloid cells, B cells, and T cells in peripheral blood, nasal, and sputum samples. In Specific Aim 2, the Adaptive Phenotyping Core will profile antigen-specific T cells in peripheral blood using a standardized workflow including an activation-induced markers (AIM)-based assay, antibody-based barcoding, and spectral flow cytometry. The antigen-specific T cells to be examined is dependent on the project with Research Project 1 focused on allergen-specific T cells and Research Project 2 focused on cit-antigen-specific T cells, autoreactive T cells implicated in the pathogenesis of rheumatoid arthritis. Importantly, each of these antigen- specific T cell populations will be analyzed side-by-side with respiratory virus-specific T cells. The Adaptive Phenotyping Core will work closely with each of the Project and Core Leads.

摘要/摘要 自适应表型核心将提供先进的实验室方法来检查整体和抗原 为重债穷国中心生成的样本的特异性免疫反应，题为“系统免疫学分析” 易受感染人群的呼吸道病毒感染”。该核心将为这两个研究项目提供服务，接收 来自临床核心的样本，并与数据管理和共享高质量的细胞概况 分析核心。自适应表型核心有两个具体目标。在具体目标 1 中，自适应 表型核心将使用光谱流式细胞术生成骨髓细胞的高维分析数据， 外周血、鼻腔和痰液样本中的 B 细胞和 T 细胞。在具体目标 2 中，自适应 表型核心将使用标准化工作流程分析外周血中的抗原特异性 T 细胞 包括基于激活诱导标记 (AIM) 的检测、基于抗体的条形码和光谱流 细胞计数术。要检查的抗原特异性 T 细胞取决于研究项目 1 的项目 专注于过敏原特异性 T 细胞，研究项目 2 专注于 cit 抗原特异性 T 细胞， 自身反应性 T 细胞参与类风湿性关节炎的发病机制。重要的是，这些抗原中的每一个 特定 T 细胞群将与呼吸道病毒特异性 T 细胞并列分析。适应性 表型核心将与每个项目和核心负责人密切合作。