Tissue-specific protein interactome mapping in a vertebrate embryo

脊椎动物胚胎中的组织特异性蛋白质相互作用组图谱

• 批准号: 10271281
• 负责人: EDWARD M MARCOTTE
• 金额: $ 19.17万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-25 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10271281
• 关键词: Actomyosin; Acute; Adhesions; Affinity Chromatography; Amphibia; Anencephaly and spina bifida X linked; Animals; Biological; Cadherins; Cells; Cellular biology; Communities; Data; Data Set; Defect; Developmental Biology; Developmental Process; Disease; Dorsal; Embryo; Fractionation; Genetic; In Vitro; Label; Lead; Light; Machine Learning; Mammals; Mass Spectrum Analysis; Mesoderm; Methods; Modeling; Molecular; Movement; Multiprotein Complexes; Neural Tube Closure; Neural Tube Defects; Post-Transcriptional Regulation; Process; Protein Interaction Mapping; Protein-Protein Interaction Map; Proteins; Proteome; Proteomics; Rana; Resources; Sampling; Spectrometry; System; Testing; Tissues; Vertebrates; Work; Xenopus; base; cell motility; convergent extension; data integration; efficacy testing; embryo tissue; experimental study; improved; in vivo; insight; novel; planar cell polarity; protein complex; protein protein interaction; skeletal dysplasia; success; vertebrate embryos

Abstract Proteins rarely act in isolation, but rather function in multi-protein complexes. Accordingly, protein-protein interactomes are exceptionally valuable resources that provide deep mechanistic insights and generate myriad hypotheses. Current methods for interactome mapping, such as affinity purification mass-spectrometry (APMS), are extremely difficult to deploy in vivo, so little comprehensive interactome data yet exists for developing embryos and even less for specific tissues within embryos. This fact poses an especially acute problem for understanding highly dynamic processes in which post-transcriptional controls dominate, for example collective cell movements. Here, we will use tissue engaged in convergent extension, a crucial collective movement that elongates the axis of animal embryos, to test the efficacy of new label-free interactome mapping approaches. Successful completion of the project will therefore be significant both for developing broadly applicable new methods and also for providing systems-level insights into a disease- relevant, vertebrate collective cell movement.

摘要 蛋白质很少单独起作用，而是以多蛋白质复合物的形式起作用。蛋白质-蛋白质 相互作用组是非常有价值的资源，它提供了深刻的机械见解，并产生了无数的 假设目前用于相互作用组作图的方法，例如亲和纯化质谱法 （APMS），在体内部署是非常困难的，所以很少有全面的相互作用组数据还存在， 发育中的胚胎，甚至更少的胚胎内的特定组织。这一事实构成了一个特别尖锐的 理解转录后控制占主导地位的高度动态过程的问题， 例如集体细胞运动。在这里，我们将使用参与会聚延伸的组织，这是一个至关重要的 一种集体运动，延长动物胚胎的轴，以测试新的无标记的功效。 相互作用基因组作图方法。因此，项目的成功完成对以下两方面都具有重要意义： 开发广泛适用的新方法，并为疾病提供系统水平的见解- 相关的脊椎动物集体细胞运动。