Development of tools for site-directed analysis of gene function

基因功能定点分析工具的开发

基本信息

  • 批准号:
    10580007
  • 负责人:
  • 金额:
    $ 76.81万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-06-01 至 2025-02-28
  • 项目状态:
    未结题

项目摘要

The goals of this application are to apply tools and refine methodologies for genome engineering in zebrafish that allow the creation of mutagenic and conditional alleles that provide spatial and temporal control of gene expression. In doing so we will be able to define genes that aid in the development and implementation of methodology to improve our understanding of genetic manipulations that can promote or restore health. The ability to make site-specific, untagged mutant alleles in zebrafish and other models has been greatly advanced by custom nucleases that include TALENs and CRISPR/Cas9 systems. These systems operate on the same principle: they are designed to bind to specific sequences in the genome and create a double strand break. During the first granting cycle, we have leveraged this ability to develop reliable methods for site-specific gene integration directed by short regions of homologous sequence. In our renewal application, we utilize the tools, vectors and methodologies generated to create both revertible alleles and Cre/Cre-ER lines for the zebrafish community in Aim 1. In Aim 2, we will examine methodologies to improve site-specific integration and the role of microhomology at CRISPR/Cas9 cut sites and new ways to present the vector with microhomology to the genomic cut site. In Aim 3, we will continue to develop and implement targeted integration resources by hosting workshops and onsite visits at both the Mayo Clinic and Iowa State University. The tools, techniques and zebrafish lines produced will have direct implications for providing precise gene editing techniques and critical lines to examine genes with the potential to restore human health. We anticipate these methodologies will enhance the efficiency of gene editing and will be readily adaptable for use in other model organisms and large animals. In our opinion, this will have important implications for modeling human disease in animal systems through the ability to utilize conditional alleles. The methods, cargos and zebrafish lines are designed to significantly enhance identification of genes that promote health through leveraging genome modification to produce conditional and revertible alleles and alleles that mirror mutations in humans.
这个应用程序的目标是应用工具和改进方法

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KARL J CLARK其他文献

KARL J CLARK的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KARL J CLARK', 18)}}的其他基金

Development of tools for site-directed analysis of gene function
基因功能定点分析工具的开发
  • 批准号:
    10187374
  • 财政年份:
    2020
  • 资助金额:
    $ 76.81万
  • 项目类别:
Development and genetics of rapid neuroendocrine stress response
快速神经内分泌应激反应的发育和遗传学
  • 批准号:
    9796476
  • 财政年份:
    2019
  • 资助金额:
    $ 76.81万
  • 项目类别:
Development and genetics of rapid neuroendocrine stress response
快速神经内分泌应激反应的发育和遗传学
  • 批准号:
    10397544
  • 财政年份:
    2019
  • 资助金额:
    $ 76.81万
  • 项目类别:
Development and genetics of rapid neuroendocrine stress response
快速神经内分泌应激反应的发育和遗传学
  • 批准号:
    10292709
  • 财政年份:
    2019
  • 资助金额:
    $ 76.81万
  • 项目类别:
Development and genetics of rapid neuroendocrine stress response
快速神经内分泌应激反应的发育和遗传学
  • 批准号:
    10389006
  • 财政年份:
    2019
  • 资助金额:
    $ 76.81万
  • 项目类别:
Development and genetics of rapid neuroendocrine stress response
快速神经内分泌应激反应的发育和遗传学
  • 批准号:
    10601205
  • 财政年份:
    2019
  • 资助金额:
    $ 76.81万
  • 项目类别:
Building the mitochondrial genome editing repertoire
构建线粒体基因组编辑库
  • 批准号:
    10447041
  • 财政年份:
    2018
  • 资助金额:
    $ 76.81万
  • 项目类别:
Building the mitochondrial genome editing repertoire
构建线粒体基因组编辑库
  • 批准号:
    10220697
  • 财政年份:
    2018
  • 资助金额:
    $ 76.81万
  • 项目类别:
Building the mitochondrial genome editing repertoire
构建线粒体基因组编辑库
  • 批准号:
    9767023
  • 财政年份:
    2018
  • 资助金额:
    $ 76.81万
  • 项目类别:
Development of tools for site-directed analysis of gene function
基因功能定点分析工具的开发
  • 批准号:
    10185650
  • 财政年份:
    2016
  • 资助金额:
    $ 76.81万
  • 项目类别:

相似海外基金

Quantification of Neurovasculature Changes in a Post-Hemorrhagic Stroke Animal-Model
出血性中风后动物模型中神经血管变化的量化
  • 批准号:
    495434
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
Bioactive Injectable Cell Scaffold for Meniscus Injury Repair in a Large Animal Model
用于大型动物模型半月板损伤修复的生物活性可注射细胞支架
  • 批准号:
    10586596
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
A Comparison of Treatment Strategies for Recovery of Swallow and Swallow-Respiratory Coupling Following a Prolonged Liquid Diet in a Young Animal Model
幼年动物模型中长期流质饮食后吞咽恢复和吞咽呼吸耦合治疗策略的比较
  • 批准号:
    10590479
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
Small animal model for evaluating the impacts of cleft lip repairing scar on craniofacial growth and development
评价唇裂修复疤痕对颅面生长发育影响的小动物模型
  • 批准号:
    10642519
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
Diurnal grass rats as a novel animal model of seasonal affective disorder
昼夜草鼠作为季节性情感障碍的新型动物模型
  • 批准号:
    23K06011
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Longitudinal Ocular Changes in Naturally Occurring Glaucoma Animal Model
自然发生的青光眼动物模型的纵向眼部变化
  • 批准号:
    10682117
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
A whole animal model for investigation of ingested nanoplastic mixtures and effects on genomic integrity and health
用于研究摄入的纳米塑料混合物及其对基因组完整性和健康影响的整体动物模型
  • 批准号:
    10708517
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
A Novel Large Animal Model for Studying the Developmental Potential and Function of LGR5 Stem Cells in Vivo and in Vitro
用于研究 LGR5 干细胞体内外发育潜力和功能的新型大型动物模型
  • 批准号:
    10575566
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
Elucidating the pathogenesis of a novel animal model mimicking chronic entrapment neuropathy
阐明模拟慢性卡压性神经病的新型动物模型的发病机制
  • 批准号:
    23K15696
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
The effect of anti-oxidant on swallowing function in an animal model of dysphagia
抗氧化剂对吞咽困难动物模型吞咽功能的影响
  • 批准号:
    23K15867
  • 财政年份:
    2023
  • 资助金额:
    $ 76.81万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了