Targeting Kruppel-like Transcription Factor for White and Grey Matter Protection in Vascular Cognitive Impairment and Dementia

针对血管认知障碍和痴呆症中白质和灰质保护的 Kruppel 样转录因子

• 批准号: 10625096
• 负责人: Kejie Yin
• 金额: $ 60.95万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10625096
• 关键词: Alzheimer' s Disease; Attenuated; Binding Sites; Blood - brain barrier anatomy; Blood Preservation; Blood Vessels; Brain; Brain Injuries; Cardiovascular system; Cells; Cerebrovascular Circulation; Cerebrovascular Disorders; Cerebrovascular Trauma; Cerebrovascular system; Cerebrum; Chronic; Cognition Disorders; Cognitive; Dementia; Development; Diabetes Mellitus; Differentiation and Growth; Diffuse; Economics; Endothelium; Extravasation; Family; Family member; Genetic; Immune; Impaired cognition; Inflammation; Inflammatory Response; Injury; Intravenous; Investigation; Kruppel-like transcription factors; Lesion; Mediating; Mediator; Molecular; Mus; Outcome; Pathogenesis; Pathologic; Peripheral; Permeability; Play; Process; Promoter Regions; Proteins; Regulation; Reporting; Research; Role; Societies; Tight Junctions; Time; Transactivation; Transgenic Organisms; Vascular Endothelium; Zinc Fingers; acute stroke; aged; blood-brain barrier disruption; blood-brain barrier permeabilization; brain endothelial cell; cell growth; cerebral hypoperfusion; cerebrovascular; cerebrovascular pathology; cognitive function; effective therapy; gray matter; hemodynamics; immune cell infiltrate; improved; member; migration; mouse model; neuron loss; novel; overexpression; preservation; prevent; social; therapeutically effective; transcription factor; transcription factor KLF13; vascular cognitive impairment and dementia; vascular contributions; white matter; white matter injury

Vascular cognitive impairment and dementia (VCID), a type of cognitive disorder mainly induced by cerebrovascular pathology and dysfunction, is widely recognized as the second most common cause of dementia after Alzheimer’s disease, and results in tremendous economic and social burdens on our society. Despite recent progress in VCID research, our understanding of cerebrovascular contributions to the pathogenesis of VCID is still limited, and the effective therapeutic approaches for VCID are unavailable. Krüppel-like factors (KLFs) are members of the zinc finger family of transcription factors and consist of 18 members that have been shown to play key roles in cellular growth and differentiation. Cumulative studies have documented that several KLFs (KLF2, KLF4, KLF5, KLF6, KLF8, KLF11, KLF13, KLF14, and KLF15) are implicated in developmental and pathological vascular processes. It is becoming apparent that KLFs are also implicated in regulating the pathogenesis of cerebrovascular diseases and warrant further investigation. KLF11 is a unique diabetes-associated KLF transcription factor among 18 KLF family members and is highly expressed in vascular endothelium. We reported for the first time that KLF11 functions as an important mediator in acute stroke-induced brain injury. However, the function and mechanisms of KLF11 in regulating cerebrovascular pathogenesis and progression of cognitive decline are totally unknown in VCID. In our recent preliminary studies, we have shown that KLF11 expression is significantly decreased in the mouse cerebral vasculature after VCID. Of note, EC-selective KLF11 transgenic overexpression displays reduced cognitive impairments in the experimental VCID mouse model, whereas KLF11 genetic deficiency results in increased cognitive impairments, white matter injury and neuronal loss. Mechanistically, we have documented that KLF11 genetic deficiency increased BBB permeability in mice after VCID. We further found several KLF11 binding sites in the promoter region of major endothelial tight junctions, and genetic deletion of KLF11 in VCID mice significantly reduced cerebral expression of claudin-5. These findings have provided the basis for our Central Hypothesis that vascular KLF11 attenuates BBB disruption and subsequent pathological cascades after chronic cerebral hypoperfusion, thereby contributing to increased BBB stabilization, reduced white matter/neuronal loss, and improved long-term cognitive outcomes in VCID. Three specific aims will be performed in this proposal. Aim 1: Define the role of vascular KLF11 in long-term cognitive disorders, brain white matter injury, and neuronal loss in experimental VCID; Aim 2: Elucidate the mechanisms of vascular KLF11-mediated brain protection in VCID; Aim 3: Explore systematic delivery of TAT- KLF11 protein as a potential therapy in VCID. Elucidating KLF11 cerebrovascular protection may help us to discover vascular contribution to brain white and grey matter injury and dementia, and lead us to develop novel and effective treatment against VCID.

血管性认知障碍与痴呆（Vascular cognitive impairment and dementia， VCID）是一种主要由