The molecular mechanism of pre-mRNA splicing
The molecular mechanism of pre-mRNA splicing
批准号:
10624937
负责人:
RUI ZHAO
金额:
$82.56万
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-06-01 至 2027-05-31
关键词:
中文摘要
总结
英文摘要
Summary
Pre-mRNA splicing is essential for gene expression in all eukaryotes and errors in splicing cause genetic
disorders and many other diseases. A thorough understanding of the molecular mechanisms of pre-mRNA
splicing has the potential to provide useful approaches for human disease therapy. The splicing of introns is
carried out through two transesterification reactions catalyzed by the spliceosome, a large RNA/protein
complex composed of five snRNPs (U1, U2, U4, U5, U6) and many non-snRNP related protein factors. The
spliceosome undergoes dramatic changes in a splicing cycle, generating the E, A, Pre-B, B, Bact, B*, C, C*, P,
and ILS complexes. Genetic, biochemical, and structural studies in the past four decades have generated
tremendous information on the mechanism of splicing, but significant knowledge gaps remain. Our goal in the
next 5 years is to use a combination of structural, biochemical, and genetic/genomics approaches to
understand the mechanism and modulation of alternative 5’ ss recognition, the mechanism of exon definition,
and the mechanism and function of transcription and splicing coupling. We envision that these projects will fill
several significant knowledge gaps and advance our understanding of the mechanism of splicing.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Selected humanization of yeast U1 snRNP leads to global suppression of pre-mRNA splicing and mitochondrial dysfunction in the budding yeast.
酵母 U1 snRNP 的选择性人源化导致芽殖酵母中前 mRNA 剪接和线粒体功能障碍的整体抑制。
DOI:
10.1101/2023.12.15.571893
发表时间:
2023
期刊:
bioRxiv : the preprint server for biology
影响因子:
--
作者:
[Chalivendra,Subbaiah, Shi,Shasha, Li,Xueni, Kuang,Zhiling, Giovinazzo,Joseph, Zhang,Lingdi, Rossi,John, Saviola,AnthonyJ, Wang,Jingxin, Welty,Robb, Liu,Shiheng, Vaeth,Katherine, Zhou,ZHong, Hansen,KirkC, Taliaferro,JMatthew, Zhao,Rui]
通讯作者:
Zhao,Rui
The molecular mechanism of pre-mRNA splicing
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批准号:10405325
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项目类别:
-
资助金额:$71.16万
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财政年份:2022
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负责人:RUI ZHAO
-
依托单位:
Structure and function of spliceosome
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批准号:10219306
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项目类别:
-
资助金额:$42.97万
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财政年份:2019
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负责人:RUI ZHAO
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依托单位:
Understanding the structure and function of U1 snRNP
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批准号:9751902
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项目类别:
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资助金额:$45.17万
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财政年份:2018
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负责人:RUI ZHAO
-
依托单位:
Understanding the structure and function of U1 snRNP
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批准号:10200085
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项目类别:
-
资助金额:$45.17万
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财政年份:2018
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负责人:RUI ZHAO
-
依托单位:
AKTA Pure Chromatography System
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批准号:9273230
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项目类别:
-
资助金额:$5.0万
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财政年份:2015
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负责人:RUI ZHAO
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依托单位:
Structure and function of U5 snRNP
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批准号:9247839
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项目类别:
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资助金额:$34.34万
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财政年份:2015
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负责人:RUI ZHAO
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依托单位:
Spinal muscular atrophy therapy using recombinant SMN proteins
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批准号:8771212
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项目类别:
-
资助金额:$19.38万
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财政年份:2014
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负责人:RUI ZHAO
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依托单位:
Understanding the structure and function of splicing factor Prp8
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批准号:7934328
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项目类别:
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资助金额:$10.86万
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财政年份:2009
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负责人:RUI ZHAO
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依托单位:
Understanding the structure and function of splicing factor Prp8
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批准号:7678577
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项目类别:
-
资助金额:$27.62万
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财政年份:2008
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负责人:RUI ZHAO
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依托单位:
PRP8, A CRITICAL PRE-MRNA SPLICING FACTOR
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批准号:7726257
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项目类别:
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资助金额:$0.61万
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财政年份:2008
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负责人:RUI ZHAO
-
依托单位:
Understanding the structure and function of splicing factor Prp8
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批准号:7916347
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项目类别:
-
资助金额:$27.26万
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财政年份:2008
-
负责人:RUI ZHAO
-
依托单位:
Understanding the structure and function of splicing factor Prp8
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批准号:8323472
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项目类别:
-
资助金额:$26.99万
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财政年份:2008
-
负责人:RUI ZHAO
-
依托单位:
Understanding the structure and function of splicing factor Prp8
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批准号:7462582
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项目类别:
-
资助金额:$27.71万
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财政年份:2008
-
负责人:RUI ZHAO
-
依托单位:
Understanding the structure and function of splicing factor Prp8
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批准号:8147670
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项目类别:
-
资助金额:$26.99万
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财政年份:2008
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负责人:RUI ZHAO
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依托单位:
PRP8, A CRITICAL PRE-MRNA SPLICING FACTOR
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批准号:7602324
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项目类别:
-
资助金额:$0.48万
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财政年份:2007
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负责人:RUI ZHAO
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依托单位:
国内基金
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