Mechanism of nerve growth factor driven axon plasticity

项目总结/摘要 轴突的可塑性，以轴突分支的产生形式，是轴突再生的基础。 神经元回路的发育和修复。在这个项目中，我们将解决的主要假设， 轴突内糖酵解途径是轴突分支形成和维持所必需的， 体外和体外。实时成像方法、分子操作、药理学和光遗传学 糖酵解酶的消融将用于确定糖酵解途径的作用。项目 还将讨论糖酵解途径和细胞骨架重塑之间的联系， 轴突分支

PROJECT SUMMARY/ABSTRACT Axon plasticity, in the form of the generation of axon branches, is fundamental to the development and repair of neuronal circuitry. In this project we will address the main hypothesis that the intra-axonal glycolytic pathway is required for the formation and maintenance of axon branches in vitro and in vitro. Live imaging approaches, molecular manipulations, pharmacology and optogenetic ablation of glycolytic enzymes will be used to determine the role of the glycolytic pathway. The project will also address the link between the glycolytic pathway and the cytoskeletal remodeling underlying axon branching.