A SARS-CoV-2 NFC ePAD Biosensor

SARS-CoV-2 NFC ePAD 生物传感器

• 批准号: 10635462
• 负责人: DAVID S DANDY
• 金额: $ 5.81万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-13 至 2023-09-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10635462
• 关键词: 2019-nCoV; Antibodies; Biosensor; COVID-19 detection; COVID-19 outbreak; COVID-19 pandemic; Carbon; Chemistry; Communicable Diseases; Communication; Development; Devices; Diagnostic; Electrodes; Future; Gold; Individual; Location; Microfluidic Microchips; Nucleocapsid Proteins; Paper; Reaction; SARS-CoV-2 antigen; Salts; Surface; Testing; Virus Diseases; Work; base; chromatin immunoprecipitation; cost; cost effective; crosslink; diagnostic assay; diagnostic platform; improved; lateral flow assay; novel diagnostics; prevent; rapid test; sensor

Proposed here is the development of an improved electrochemical sensor for detection of the SARS-CoV-2 nucleocapsid protein using thermoplastic electrodes (TPEs). The need for faster testing amid the COVID-19 pandemic brought about several alternatives to RT-qPCR testing for viral infections. However, current rapid tests are lacking in sensitivity (lateral flow assays and other paper based tests) or expensive (gold based electrochemical tests). TPEs solve both the sensitivity and cost issues and have been shown to be more robust than stencil printed carbon electrodes (SPCE). The TPE surfaces will be covalently modified using electroreduction of aryldiazonium salts followed by “click chemistry” to provide a platform for simple antibody crosslinking reactions. The fully characterized and functional sensor will then be the basis for future work integrating it into a microfluidic device for a full on-chip assay.

提出了一种改进的电化学传感器的研制，用于检测空气中的 使用热塑性电极(TPE)检测SARS-CoV-2核衣壳蛋白。对更快的需求 新冠肺炎大流行期间的检测带来了RT-qPCR检测的几个替代方案 病毒感染。然而，目前的快速检测缺乏敏感性(侧向流动分析和 其他纸基测试)或昂贵的(金基电化学测试)。TPE既解决了 敏感性和成本问题，并已被证明比模版印刷碳更坚固 电极(SPCE)。热塑性弹性体表面将通过电还原进行共价修饰 芳基重氮盐后接“点击化学”为简单抗体提供平台 交联化反应。然后，充分表征和功能的传感器将成为 未来的工作是将其集成到微流控设备中进行全面的芯片分析。

项目成果

Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.

• DOI: 10.1021/acsmeasuresciau.2c00037
• 发表时间: 2022-12-21
• 期刊: ACS MEASUREMENT SCIENCE AU
• 作者: Clark, Kaylee M.;Schenkel, Melissa S.;Pittman, Trey W.;Samper, Isabelle C.;Anderson, Loran B. R.;Khamcharoen, Wisarut;Elmegerhi, Suad;Perera, Rushika;Siangproh, Weena;Kennan, Alan J.;Geiss, Brian J.;Dandy, David S.;Henry, Charles S.
• 通讯作者: Henry, Charles S.

Characterization of Factors Affecting Stripping Voltammetry on Thermoplastic Electrodes.

影响热塑性电极溶出伏安法的因素的表征。

• DOI: 10.1149/1945-7111/acfa68
• 发表时间: 2023
• 期刊: Journal of the Electrochemical Society
• 影响因子: 3.9
• 作者: McMahon,CatherineJ;Martinez,Brandaise;Henry,CharlesS
• 通讯作者: Henry,CharlesS