B Cell/T Cell Interactions in Brucellosis

布鲁氏菌病中 B 细胞/T 细胞的相互作用

• 批准号: 10630491
• 负责人: Jerod Skyberg
• 金额: $ 5.01万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-11-18 至 2023-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630491
• 关键词: Affect; Animals; Anti-Inflammatory Agents; Antibiotic Therapy; Antibodies; Antigen Presentation; Antigen-Presenting Cells; Award; B-Lymphocytes; Brucella; Brucella Vaccine; Brucellosis; CD4 Positive T Lymphocytes; Cell Communication; Cell Separation; Cell physiology; Cells; Chronic; Complement; Control Animal; Data; Disease; Fever; Future; Gene Expression Profiling; Genetic Transcription; Goals; Human; Immunity; Impairment; Infection; Knowledge; Laboratories; Ligands; Mediating; Missouri; Modeling; Mus; Pathogenesis; Pathway interactions; Predisposition; Public Health; Relapse; Role; T cell response; T-Lymphocyte; Universities; Vaccinated; Vaccination; Vaccine Design; Vaccines; Virulent; Zoonoses; cytokine; design; improved; neglect; prevent; rational design; receptor; response; transcriptome sequencing; vaccine access; vaccine development

Project Summary/Abstract In humans, Brucella spp. can cause a lifelong, debilitating disease, with relapses of undulating fever and other complications even with antibiotic treatment. No vaccines are currently licensed to prevent human brucellosis. In this focused Supplement, we will employ the BSL3 cell sorting capability of the University of Missouri Regional Biocontainment Laboratory (MURBL) along with RNA-seq to perform detailed transcriptional analyses of B and T cells in our model of vaccine-mediated immunity to Brucella. Our findings from this Supplement will complement the results from our parental proposal and provide us additional information which will enhance our understanding of how B cell antigen presentation inhibits vaccine-mediated immunity against Brucella, which in turn could improve the rational design of vaccines for brucellosis.

项目总结/摘要 在人类中，布鲁氏菌属（Brucella spp.）可能导致终身衰弱的疾病，反复出现波动性发热， 其他并发症，甚至抗生素治疗。目前没有疫苗被许可用于预防人类 布鲁氏菌病在这个重点补充，我们将采用BSL 3细胞分选能力的大学， 密苏里州区域生物防护实验室（MURBL）沿着与RNA-seq一起进行详细的转录 我们的疫苗介导的布鲁氏菌免疫模型中的B和T细胞分析。我们的发现 补充资料将补充我们的父母建议的结果，并为我们提供额外的信息， 将增强我们对B细胞抗原呈递如何抑制疫苗介导的抗 这反过来又可以改善布鲁氏菌病疫苗的合理设计。