B Cell/T Cell Interactions in Brucellosis

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Project Summary/Abstract In humans, Brucella spp. can cause a lifelong, debilitating disease, with relapses of undulating fever and other complications even with antibiotic treatment. No vaccines are currently licensed to prevent human brucellosis. In this focused Supplement, we will employ the BSL3 cell sorting capability of the University of Missouri Regional Biocontainment Laboratory (MURBL) along with RNA-seq to perform detailed transcriptional analyses of B and T cells in our model of vaccine-mediated immunity to Brucella. Our findings from this Supplement will complement the results from our parental proposal and provide us additional information which will enhance our understanding of how B cell antigen presentation inhibits vaccine-mediated immunity against Brucella, which in turn could improve the rational design of vaccines for brucellosis.