Bone Cells Analysis

骨细胞分析

• 批准号: 10626813
• 负责人: PAOLA DIVIETI PAJEVIC
• 金额: $ 24.14万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-19 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626813
• 关键词: 3-Dimensional; Ablation; Animal Model; Architecture; Biological Assay; Biological Markers; Biology; Blood; Boston; Cartilage; Cell Line; Cell Separation; Cells; Clinical; Collaborations; Communities; Consultations; Cost efficiency; Cultured Cells; Cyclic AMP; DNA Sequencing Facility; Data; Education; Educational process of instructing; Educational workshop; Endocrine; Fees; Fostering; Funding; Genes; Genetic; Genetically Modified Animals; Goals; Human; In Situ Hybridization; In Vitro; Informatics; Leadership; Libraries; Measurement; Mentors; Methodology; Methods; Mission; Morphology; Mus; Pediatric Hospitals; Performance; Population; Process; Property; Protocols documentation; RNA; RNA analysis; Reagent; Recovery; Research; Research Design; Research Personnel; Resources; Route; Sampling; Scientific Advances and Accomplishments; Series; Services; Signal Pathway; Signal Transduction; Signal Transduction Pathway; Signaling Molecule; Specimen; Structure; System; Techniques; Technology; Tissues; Total Hip Replacement; Training; Training and Education; Universities; Urine; Work; bone; bone cell; cell preparation; cost; cost effective; design; established cell line; experimental study; genetic analysis; genetic manipulation; human subject; in vivo; innovation; instrumentation; interest; investigator training; molecular dynamics; novel; operation; pre-clinical; programs; quality assurance; scaffold; single-cell RNA sequencing; skeletal; skeletal tissue; skills; tissue preparation; tool; trafficking; transcriptome sequencing; transcriptomics

The proposed Bone Cells Analysis Core (BCAC) will enable participating investigators to pursue new lines of research in skeletal biology by using state-of-the-art technologies for bone cell isolation, functional assessment, and RNA sequencing. The proposed Core will bring together the expertise of its leadership (Director Dr. Pajevic Divieti and the Associate Directors Drs. Gardella, Gerstenfeld and Warman) and powerful new research approaches and assay systems to make for a synergistic Core Resource that will greatly enrich and catalyze the scientific advances of participating investigators. The BCAC will provide Center investigators (CIs) with 1) Preclinical and clinical bone cells and genetic tools, 2) Cell Signaling and Biomarker Assay services, and 3) RNA sequencing services. The Bone cell Core (Drs Divieti Pajevic and Gerstenfeld, Boston University), will provide mouse- derived bone cells and cells lines from both wild type or genetically modified animals. The Core will also provide, in collaboration with Dr. Gerstenfeld (Associate Director), highly annotated human specimens from total hip replacements. The BCAC will offer a wide array of in-house and commercially available signaling assays under the Signaling Assay service (Dr. Gardella, Associate Director, Endocrine Unit, MGH). The Core will help with the study design and will train investigators in all the aspects of these techniques. The RNA sequencing core (Dr. Warman, Associate Director, Boston Children’s Hospital) will provide services to help CIs and their trainees design, perform, and analyze bulk and single cell RNA sequencing experiments that involve skeletal cells and tissues. The Core will provide help with designing bulk and single cell RNA sequencing experiments and with assisting with tissue and cell preparation for RNA recovery. The core will provide micro-pulverizers and optimized methods for recovering RNA from bone and cartilage for bulk RNA sequencing and living cells for single cell RNA sequencing. The Core will generate sequencing libraries using kits designed for very low amounts of starting RNA. The Core will assist in the informatics analysis of the sequencing data. Each service provided by the Core will be rigorously controlled for quality performance and reliability. The Core will conduct hands-on training sessions by which investigators can be mentored and taught how to perform assays, cell manipulations on their own and RNA sequencing. It will also establish a seminar series in which new methodologies, approaches, and related scientific progress will be presented and discussed. !

拟议的骨细胞分析核心（BCAC）将使参与的研究人员能够追求 通过使用最先进的骨细胞技术， 分离、功能评估和RNA测序。拟议的核心将汇集 领导层的专业知识（主任Pajevic Divieti博士和副主任Gardella博士， Gerstenfeld和沃曼）和强大的新的研究方法和分析系统，使 一个协同核心资源，将大大丰富和催化科学进步， 参与调查者。 BCAC将为中心研究者（CI）提供1）临床前和临床骨细胞， 遗传工具，2）细胞信号和生物标志物分析服务，以及3）RNA测序服务。 骨细胞核心（Drs Divieti Pajevic和Gerstenfeld，波士顿大学）将提供小鼠- 来源于野生型或遗传修饰动物的骨细胞和细胞系。芯会 还提供，与Gerstenfeld博士（副主任）合作，高度注释人类 全髋关节置换术的标本。BCAC将提供广泛的内部和 在SignalingAssay服务下的市售信号测定（Gardella博士， 副主任，内分泌单位，MGH）。核心将帮助研究设计，并将培训 研究人员在这些技术的各个方面。RNA测序核心（沃曼博士， 波士顿儿童医院副院长）将提供服务，帮助CI及其学员 设计、执行和分析批量和单细胞RNA测序实验，包括 骨骼细胞和组织。核心将为设计批量和单细胞RNA提供帮助 测序实验以及协助组织和细胞制备以回收RNA。的 核心将提供微粉碎机和优化的方法，从骨骼中回收RNA， 软骨用于批量RNA测序，活细胞用于单细胞RNA测序。芯会 使用设计用于非常低量的起始RNA的试剂盒产生测序文库。核心 将有助于测序数据的信息学分析。 由核心提供的每项服务都将严格控制质量性能， 可靠性核心小组将举办实践培训班， 指导和教导如何进行分析，细胞操作自己和RNA测序。 它还将举办一系列研讨会， 将介绍和讨论科学进展。 !