Immunopathology Core

免疫病理学核心

• 批准号: 10628215
• 负责人: Rudragouda Channappanavar
• 金额: $ 13.01万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-05 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10628215
• 关键词: Anatomy; Animals; Area; Awareness; Biomedical Research; Cell Separation; Cells; Centers of Research Excellence; Collaborations; Communicable Diseases; Communities; Comparative Study; Complex; Core Facility; Data; Development; Elements; Extramural Activities; Fee-for-Service Plans; Fees; Flow Cytometry; Fluorescence-Activated Cell Sorting; Foundations; Funding; Goals; Grant; Growth; Histologic; Histopathology; Human Resources; Immune System Diseases; Immunoassay; Immunofluorescence Immunologic; Immunohistochemistry; Immunologics; Immunology; Immunology procedure; In Vitro; Incentives; Infrastructure; Institution; International; Laboratories; Lesion; Oklahoma; Pathogenesis; Pathologic; Pathology; Phase; Pilot Projects; Research; Research Design; Research Personnel; Research Project Grants; Research Support; Resources; Respiratory Center; Respiratory Disease; Scientist; Services; System; Technology; Tissue Model; Tissues; Training; assay development; cell analyzer; data curation; experience; immunopathology; in vivo; instrumentation; outreach; outreach program; programs; recruit; service programs; success; symposium; tissue processing; user-friendly; voucher

Immunopathology Core Project Summary Biomedical researchers are often limited in their ability to perform complex immunopathologic analyses, either by a lack of technology/instrumentation available or by inexperience in delineating complex mechanisms associated with immune dysfunction and disease pathogenesis. The Immunopathology Core Laboratory (IPC) program in the Oklahoma Center of Respiratory and Infectious Diseases (OCRID) bridges this gap and provides a comprehensive solution by providing (1) access to cutting-edge assays for immunological data curation, (2) tissue processing for histopathology and downstream immunologic assays, and (3) expert support in the analyses of immunopathologic data and gross/histologic lesion interpretation. At its core, the IPC functions as a critical resource for pilot projects and OCRID investigators by providing flow cytometry, fluorescent-assorted cell sorting (FACS), immunofluorescent immunohistochemistry (IFA-IHC), and multiplex immunoassay (MIA) analyses. These functions are augmented through integration of our histopathology unit, which provides essential support in processing of research tissues for pathologic interpretation and downstream immunologic analyses. The primary function of the IPC in Phase III of this will be to provide continued immunology and pathology support for COBRE pilot projects and OCRID Center Investigators through implementation of a voucher program. The immediate impacts of the IPC in Phase III will be to (1) sustain OCRID pilot projects and investigators by facilitating access to immunopathologic services, and (2) continue development of OCRID by providing the means to incentivize growth of OCRID programs and projects beyond Phase III. The long-term goal of this Phase III project will be to broaden and diversify the IPC to recruit investigators from wider research fields from supporting institutions, thereby instituting a long-term solution for researchers in the region. To achieve this, the IPC will capitalize on the momentum gained by our fee-for-service program to expand services and capabilities to serve a larger group of scientists in the regional research community through (1) outreach seminars to increase awareness across the region, (2) soliciting collaborative extramural grant collaborations to recruit projects that utilize core services, and (3) acquisition of a user-friendly, high throughput cell analyzer to facilitate usage by wider clientele. We have already taken steps to increase usage by OCRID investigators and researchers at other institutions and by implementing outreach programs to increase our profile and build intra- and extramural clientele within the region, we will expand the IPC into a self-sustaining entity, able to support current and long-term state-of-the-art research projects at local and regional institutions.

免疫病理学核心 项目摘要 生物医学研究人员通常在执行复杂的免疫病理分析方面也受到限制 由于缺乏可用的技术/仪器或在描述复杂机制方面缺乏经验 与免疫功能障碍和疾病发病机制有关。免疫病理学核心实验室(IPC) 俄克拉荷马州呼吸和传染病中心(OCRID)的计划弥合了这一差距，并提供了 通过提供(1)用于免疫学数据管理的尖端化验的综合解决方案，(2) 组织病理学和下游免疫学分析的组织处理，以及(3)在 免疫病理资料分析和大体/组织学病变解释。就其核心而言，IPC的功能是 为试点项目和OCRID调查人员提供关键资源，通过提供流式细胞术、荧光分类细胞 分选(FACS)、免疫荧光免疫组织化学(IFA-IHC)和多重免疫分析(MIA) 分析。通过整合我们的组织病理学部门，这些功能得到了增强，这提供了 为病理解释和下游免疫学研究组织的处理提供必要的支持 分析。IPC在第三阶段的主要职能将是提供持续的免疫学和 对Cobre试点项目和OCRID中心调查人员通过实施 代金券计划。IPC在第三阶段的直接影响将是：(1)维持OCRID试点项目和 通过便利获得免疫病理服务，以及(2)通过以下方式继续发展OCRID 提供手段以激励OCRID方案和项目在第三阶段之后的增长。长期 这一第三阶段项目的目标将是扩大IPC并使其多样化，以便从更广泛的研究中招募调查人员 从支助机构获得的研究领域，从而为该地区的研究人员制定了一项长期解决方案。至 实现这一目标，IPC将利用我们的按服务收费计划获得的势头来扩大服务 以及通过(1)外联为区域研究界更大的科学家群体提供服务的能力 举办研讨会，提高整个区域的认识；(2)征集合作的校外赠款合作，以 招募利用核心服务的项目，以及(3)购买用户友好的高通量细胞分析仪，以 方便更广泛的客户使用。我们已经采取措施增加OCRID调查人员和 其他机构的研究人员，并通过实施外联计划来提高我们的形象并建立内部 和区域内的外部客户，我们将把IPC扩大为一个自我维持的实体，能够支持 地方和区域机构的当前和长期最先进的研究项目。