Immunopathology Core

免疫病理学核心

• 批准号: 10628215
• 负责人: Rudragouda Channappanavar
• 金额: $ 13.01万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-05 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10628215
• 关键词: Anatomy; Animals; Area; Awareness; Biomedical Research; Cell Separation; Cells; Centers of Research Excellence; Collaborations; Communicable Diseases; Communities; Comparative Study; Complex; Core Facility; Data; Development; Elements; Extramural Activities; Fee-for-Service Plans; Fees; Flow Cytometry; Fluorescence-Activated Cell Sorting; Foundations; Funding; Goals; Grant; Growth; Histologic; Histopathology; Human Resources; Immune System Diseases; Immunoassay; Immunofluorescence Immunologic; Immunohistochemistry; Immunologics; Immunology; Immunology procedure; In Vitro; Incentives; Infrastructure; Institution; International; Laboratories; Lesion; Oklahoma; Pathogenesis; Pathologic; Pathology; Phase; Pilot Projects; Research; Research Design; Research Personnel; Research Project Grants; Research Support; Resources; Respiratory Center; Respiratory Disease; Scientist; Services; System; Technology; Tissue Model; Tissues; Training; assay development; cell analyzer; data curation; experience; immunopathology; in vivo; instrumentation; outreach; outreach program; programs; recruit; service programs; success; symposium; tissue processing; user-friendly; voucher

Immunopathology Core Project Summary Biomedical researchers are often limited in their ability to perform complex immunopathologic analyses, either by a lack of technology/instrumentation available or by inexperience in delineating complex mechanisms associated with immune dysfunction and disease pathogenesis. The Immunopathology Core Laboratory (IPC) program in the Oklahoma Center of Respiratory and Infectious Diseases (OCRID) bridges this gap and provides a comprehensive solution by providing (1) access to cutting-edge assays for immunological data curation, (2) tissue processing for histopathology and downstream immunologic assays, and (3) expert support in the analyses of immunopathologic data and gross/histologic lesion interpretation. At its core, the IPC functions as a critical resource for pilot projects and OCRID investigators by providing flow cytometry, fluorescent-assorted cell sorting (FACS), immunofluorescent immunohistochemistry (IFA-IHC), and multiplex immunoassay (MIA) analyses. These functions are augmented through integration of our histopathology unit, which provides essential support in processing of research tissues for pathologic interpretation and downstream immunologic analyses. The primary function of the IPC in Phase III of this will be to provide continued immunology and pathology support for COBRE pilot projects and OCRID Center Investigators through implementation of a voucher program. The immediate impacts of the IPC in Phase III will be to (1) sustain OCRID pilot projects and investigators by facilitating access to immunopathologic services, and (2) continue development of OCRID by providing the means to incentivize growth of OCRID programs and projects beyond Phase III. The long-term goal of this Phase III project will be to broaden and diversify the IPC to recruit investigators from wider research fields from supporting institutions, thereby instituting a long-term solution for researchers in the region. To achieve this, the IPC will capitalize on the momentum gained by our fee-for-service program to expand services and capabilities to serve a larger group of scientists in the regional research community through (1) outreach seminars to increase awareness across the region, (2) soliciting collaborative extramural grant collaborations to recruit projects that utilize core services, and (3) acquisition of a user-friendly, high throughput cell analyzer to facilitate usage by wider clientele. We have already taken steps to increase usage by OCRID investigators and researchers at other institutions and by implementing outreach programs to increase our profile and build intra- and extramural clientele within the region, we will expand the IPC into a self-sustaining entity, able to support current and long-term state-of-the-art research projects at local and regional institutions.

免疫病理学核心 项目摘要 生物医学研究人员进行复杂的免疫病理学分析的能力往往有限， 由于缺乏可用的技术/仪器或由于在描述复杂机制方面缺乏经验， 与免疫功能障碍和疾病发病机制相关。免疫病理学核心实验室（IPC） 俄克拉荷马州呼吸道和传染病中心（OCRID）的一个项目填补了这一空白， 全面的解决方案，提供（1）获得用于免疫学数据管理的尖端检测方法，（2） 用于组织病理学和下游免疫学测定的组织处理，以及（3）在 免疫病理学数据分析和大体/组织学病变解释。IPC的核心功能是 通过提供流式细胞术、荧光分类细胞仪和荧光标记细胞， 分选（FACS）、免疫荧光免疫组织化学（IFA-IHC）和多重免疫测定（MIA） 分析。这些功能通过我们的组织病理学单元的整合得到增强， 为病理学解释和下游免疫学研究提供研究组织处理的必要支持 分析。IPC在III期的主要功能是提供持续的免疫学和 为COBRE试点项目和OCRID中心研究人员提供病理学支持， 代金券计划。IPC在第三阶段的直接影响将是（1）维持OCRID试点项目， 研究者通过促进获得免疫病理学服务，和（2）继续发展OCRID， 提供激励OCRID计划和项目在第三阶段之后发展的手段。长期 第三阶段项目的目标是扩大IPC的范围，使其多样化，以便从更广泛的研究中招募研究人员 从支持机构获得的研究领域，从而为该地区的研究人员制定了长期解决方案。到 为了实现这一目标，IPC将利用我们的收费服务计划所获得的势头来扩大服务 以及通过以下方式为区域研究界更多科学家提供服务的能力：（1）外联 研讨会，以提高整个地区的认识，（2）征求合作校外赠款合作， 招募利用核心服务的项目，以及（3）获得用户友好的高通量细胞分析仪， 方便广大客户使用。我们已经采取措施增加OCRID调查员的使用， 研究人员在其他机构，并通过实施外展计划，以提高我们的形象和建立内部， 和该地区的校外客户，我们将把IPC扩展为一个自我维持的实体，能够支持 在地方和区域机构开展当前和长期的最先进的研究项目。