Neuroimaging and Electrophysiology Facility (NIEF)

神经影像和电生理学设施 (NIEF)

• 批准号: 10628976
• 负责人: JORGE David MIRANDA
• 金额: $ 43.35万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10628976
• 关键词: Academia; Academic degree; Anesthesia procedures; Animals; Applications Grants; Area; Biological; Biophysics; Brain; Calcium; Caribbean region; Cells; Centers of Research Excellence; Collaborations; Communities; Computer software; Core Facility; Cultured Cells; Data; Data Analyses; Development; Educational workshop; Electrophysiology (science); Equipment; Faculty; Fee-for-Service Plans; Fluorescence Microscopy; Funding; Generations; Goals; Grant; Human Resources; Image; Industry; Institution; Island; Laboratories; Legal patent; Manuscripts; Microscope; Microscopy; Mission; Molecular; Monitor; Neurobiology; Neuronal Plasticity; Oocytes; Outcome; Participant; Peer Review; Phase; Postdoctoral Fellow; Privatization; Procedures; Productivity; Publications; Publishing; Puerto Rico; Research; Research Infrastructure; Research Personnel; Research Project Grants; Scanning; Science; Scientist; Services; Slice; Students; Techniques; Technology; Training; Universities; Visit; data acquisition; environmental enrichment for laboratory animals; experience; experimental study; faculty support; graduate student; innovation; instrument; instrumentation; interest; investigator training; neuroimaging; new technology; research facility; role model; service providers; tool; two-photon; ultra high resolution; undergraduate student; voltage

Project Summary: The Neuroimaging and Electrophysiology Facility (NIEF) was established to increase the competitiveness of scientists in Puerto Rico via the incorporation of microscopy and electrophysiology techniques in their research projects. This aim was achieved by the establishment of two research cores, one at the Molecular Science Research Center (MSRC) and another at the Institute of Neurobiology (INB), from the University of Puerto Rico. Both facilities support a diversified group of investigators on the island. During phases 1 and 2 of COBRE, the NIEF acquired state-of-the-art instruments that are expensive and not accessible in most laboratories, departments and/or institutions. Among the equipment obtained or supervised by the NIEF are confocal, two-photon, and super resolution microscopes. Also available are electrophysiology rigs for oocytes, cultured cells, brain slice, and anesthetized animals to monitor cell electrical activity. As a result of the NIEF research infrastructure and expert technical personnel, 113 peer reviewed manuscripts have been published, leading to 1,816 citations. Moreover, data obtained from the NIEF cores were included in 84 grant proposals that were submitted to federal, state, and private agencies, and from those submitted, 48 were funded. Furthermore, research performed in the NIEF permitted the innovation of new technology by supporting investigators with the development of 7 patents. Finally, over 50 graduate students from eight different departments and three main research institutions have benefitted from the NIEF cores by being able to complete at least one of their theses aims and attain their academic degrees, thus demonstrating the impact of the NIEF in the scientific community of Puerto Rico. These results confirm the beneficial impact of the NIEF cores on the investigators in the island. Therefore, in this COBRE phase 3 proposal, the goal is to maintain, strengthen, and support the NIEF cores by implementing three aims: 1) to provide scientists in Puerto Rico with state-of-the-art instrumentation in microscopy and electrophysiology, 2) to support the investigators in the island with expert staff that will help them with the acquisition and analysis of the data from microscopy and electrophysiology techniques, and 3) to train researchers in several technologies associated with these two approaches (microscopy and electrophysiology) via seminars, workshop, and hands-on training. Thus, the facilities and services will continue to help investigators use the most innovative electrophysiological and microscopy technology to generate interdisciplinary projects to study biological and biophysical phenomena under the guidance of experienced and excellent staff. Fulfillment of these aims will enable a transition of the NIEF to a completely self-sustained facility beyond COBRE phase 3 and a role model for Puerto Rico and the Caribbean.

项目概要： 建立了神经影像和电生理设施（NIEF），以提高竞争力 波多黎各的科学家们通过将显微镜和电生理学技术结合到他们的 研究项目。这一目标是通过建立两个研究核心来实现的，一个在分子生物学中心， 科学研究中心（MSRC）和另一个在神经生物学研究所（INB），从大学 来自波多黎各。这两个设施都支持岛上的各种调查人员。第1阶段期间 和COBRE的2，NIEF获得了最先进的仪器，这些仪器价格昂贵， 大多数实验室、部门和/或机构。在获得或监督的设备中， NIEF是共焦、双光子和超分辨率显微镜。也可利用电生理学 用于卵母细胞、培养细胞、脑切片和麻醉动物的装置，以监测细胞电活动。作为 由于NIEF的研究基础设施和专家技术人员，113同行评审的手稿 已发表，被引用1，816次。此外，从NIEF核心获得的数据是 包括在提交给联邦、州和私人机构的84份赠款提案中， 有48人获得资助。此外，在NIEF进行的研究允许新的创新 通过支持研究人员开发7项专利来支持技术。最后，50多名毕业生 来自八个不同部门和三个主要研究机构的学生受益于 NIEF的核心是能够完成至少一个论文目标并获得学位， 从而证明了NIEF在波多黎各科学界的影响。这些结果 确认NIEF核心对岛上研究人员的有益影响。因此，在这个COBRE 第三阶段的建议，目标是通过实施三个方面来维护、加强和支持国家信息经济框架的核心 目的：1）为波多黎各的科学家提供最先进的显微镜仪器， 电生理学，2）支持岛上的调查人员与专家工作人员，这将有助于他们与 采集和分析来自显微镜和电生理技术的数据，以及3）训练 研究人员在与这两种方法相关的几种技术（显微镜和 电生理学），通过研讨会，讲习班，和动手培训。因此，设施和服务将 继续帮助研究人员使用最具创新性的电生理和显微镜技术， 产生跨学科的项目，研究生物和生物物理现象的指导下， 经验丰富的优秀员工。实现这些目标将使NIEF过渡到一个 COBRE第3阶段之后完全自我维持的设施，是波多黎各和 加勒比海