Human Tissues, Lipidomics, and Proteomics Core

摘要-人体组织、脂蛋白、脂质组学和蛋白质组学核心(C2) 人类组织、脂蛋白、脂质组学和蛋白质组学核心(核心2，C2)将集中在 为所有三个项目提供人体组织和分析能力，以提高可译性 在P1-P3中提出的小鼠研究在人类临床实践中的应用。同时，酷睿2将 提供我们的机构目前没有提供的脂蛋白、脂组和蛋白质组分析 代谢组学核心。心血管研究界已经证明了许多重要的 高血压患者血浆致动脉粥样硬化颗粒水平与临床结局的相关性 不同的密度，由超速离心法测定。更详细的分析现在 发现这些颗粒中存在心血管疾病(CVD)的额外风险，例如 载脂蛋白C3和神经酰胺含量。对于此PPG，酷睿2将提供更细粒度的分析 离心法和尺寸排阻层析法分离脂蛋白 并对这些颗粒进行无偏见的脂组和蛋白质组分析。此外，临床样本 由核心主任获得的(血清、肝脏、脂肪)将在所有三个月提供给调查人员 项目。这些服务将避免PPG调查人员维护所需的 在自己的实验室里使用仪器，或者使用昂贵的商业服务。通过集中管理 和标准化程序，Core 2提供了一组通用的分析工具，以提供 对高血压病病理生理过程分子机制的统一认识 动脉粥样硬化，巨噬细胞生物学，富含甘油三酯的脂蛋白的调节，以及肥胖。通过 与核心1提供的服务相辅相成和整合，核心2员工还提供 生物信息学支持分析和解释蛋白质组和脂肪组数据集，使核心 PPG调查人员在这些方面几乎没有背景或专业知识的情况下可以使用2种能力 分析技术。一个关键的长期目标是整合脂肪组学的定量分析 为糖尿病相关心血管疾病提供系统生物学观点的功能研究数据 疾病。CORE 2还将开发适合特定研究目标的新分析方法 PPG调查员。具体地说，我们将获得并使用标准来量化和发现 候选脂类在动脉粥样硬化中的作用正在研究中。这些方法将是 被PPG调查人员广泛使用。核心2将以最佳效率提供这些服务 和成本效益，避免了个人在 PPG。

SUMMARY – HUMAN TISSUES, LIPOPROTEINS, LIPIDOMICS, & PROTEOMICS CORE (C2) The Human Tissues, Lipoproteins, Lipidomics, and Proteomics Core (Core 2, C2) will focus on providing human tissues and analytical capabilities to all three projects to enhance translatability of mouse studies proposed in P1–P3 to clinical practice in humans. In parallel, Core 2 will provide lipoprotein, lipidomic, and proteomic analyses currently not provided by our institutional Metabolomics Core. The cardiovascular research community has shown many important correlations between clinical outcomes and the plasma levels of atherogenic particles of different densities, as determined by ultracentrifugation. More detailed analyses are now uncovering additional risk for cardiovascular disease (CVD) within these particles, such as the content of apoC3 and ceramides. For this PPG, Core 2 will provide a more granular analysis of lipoproteins isolated by centrifugation and size exclusion chromatography through both directed and unbiased lipidomic and proteomic analyses of these particles. In addition, clinical samples (sera, liver, adipose) obtained by the core director will be provided to investigators in all three projects. These services will avoid the need for PPG investigators to maintain the required instrumentation in their own laboratories or use expensive commercial services. By centralizing and standardizing procedures, Core 2 provides a common set of analytical tools to provide a unified understanding of molecular mechanisms involved in pathophysiologic processes of atherosclerosis, macrophage biology, regulation of triglyceride-rich lipoproteins, and obesity. By complementing and integrating with the service provided by Core 1, Core 2 staff also provide bioinformatics support to analyze and interpret proteomic and lipidomic data sets, making Core 2 capabilities available to PPG investigators with little background or expertise in these analytical technologies. A key long-term goal is to integrate quantitative analyses of lipidomic data with functional studies to provide a systems biology view of diabetes-related cardiovascular disease. Core 2 will also develop new analytical methods tailored to specific research objectives of PPG investigators. Specifically, we will obtain and use standards to quantify and discover candidate lipids whose role in atherosclerosis is being investigated. These methods will be extensively used by PPG investigators. Core 2 will offer these services with optimal efficiency and cost-effectiveness, avoiding the need for individuals to pursue such analyses outside the PPG.