The electrophysiology of biofilm development and drug resistance
生物膜发育和耐药性的电生理学
基本信息
- 批准号:10740493
- 负责人:
- 金额:$ 13.87万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-07-06 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAdjuvantAminoglycoside resistanceAminoglycosidesAntibioticsBacteriaBasic ScienceBiological AssayBiotechnologyCOVID-19CalciumCell SurvivalCellsCessation of lifeChemical StructureClinicClinicalCombined AntibioticsComplexDNA Sequence AlterationDNA biosynthesisDataDevelopmentDimensionsDrug CombinationsDrug SynergismDrug resistanceElectrophysiology (science)Gene CombinationsGentamicinsGrowthHospitalsInfectionInterventionLife Cycle StagesMachine LearningMeasurementMeasuresMechanical StimulationMechanicsMediatingMetabolicMethodsMicrobial BiofilmsMorphologyOpticsOutcomePharmaceutical PreparationsPhasePhysiologicalPhysiologyPositioning AttributePostdoctoral FellowPredictive ValueProbioticsRefractoryResistanceRoleSignal PathwaySpeedSuspensionsTechniquesTechnologyTestingTherapeuticTrainingUrinary tract infectionWorkantibiotic resistant infectionsantibiotic tolerancebactericidecell motilitycombatcombinatorialcommunedeep learningdeep learning modeldensitydrug resistant pathogenhigh throughput screeningin silicoinnovationinsightinventionknockout genemachine learning modelminimal inhibitory concentrationmulti-electrode arraysnew technologynon-geneticnovel drug combinationnovel therapeutic interventionpre-clinicalpreemptscreeningsensorsoft tissuesynergismtechnology developmenttreatment strategy
项目摘要
Project Summary
Biofilms are intrinsically drug-resistant. While the signaling pathways mediating resistance in biofilms are documented,
only recently has the importance of electrophysiology in modifying gentamicin resistance come to light. However, the role
of electrophysiology in biofilm formation and maturation is unknown. Furthermore, we currently lack high throughput
screening techniques for assessing the bactericidal potential of treatment strategies against clinical biofilms.
During my K99 training, I will investigate how mechanically-stimulated calcium fluctuations modify the c-di-GMP pools
(Aim 1.1) and bacterial swarming (Aim 1.2) leading to biofilm formation. Concomitantly, I will deploy an assay for measur-
ing biofilm viability in high throughput to screen for antibiotic adjuvants against biofilms (Aim 2.1). During this phase, my
training will focus on the culture of biofilms, analysis of non-optical electrophysiology data, and building machine learning
models subsequently applied during my R00 phase.
Following my K99 phase, I will investigate the electrophysiology of mature biofilms using both optical and non-optical
techniques and correlate changes in antibiotic tolerance across the biofilm life-cycle with changes in electrophysiology (Aim
1.3). Concomitantly, I will build and train deep learning models to predict gentamicin adjuvants against slow-growing cells
based on viability data collected in my K99 phase (Aim 2.2).
The product of my project will enrich our understanding of biofilm electrophysiology and enable methods for mitigating
their formation or promoting their retention as is desirable for probiotics. Additionally, this work will deliver new machine
learning technologies for finding antibiotic adjuvants to combat drug-resistant clinical biofilms.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Christian T Meyer其他文献
Christian T Meyer的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似海外基金
Metachronous synergistic effects of preoperative viral therapy and postoperative adjuvant immunotherapy via long-term antitumor immunity
术前病毒治疗和术后辅助免疫治疗通过长期抗肿瘤免疫产生异时协同效应
- 批准号:
23K08213 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Improving the therapeutic immunity of cancer vaccine with multi-adjuvant polymeric nanoparticles
多佐剂聚合物纳米粒子提高癌症疫苗的治疗免疫力
- 批准号:
2881726 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
Studentship
Countering sympathetic vasoconstriction during skeletal muscle exercise as an adjuvant therapy for DMD
骨骼肌运动期间对抗交感血管收缩作为 DMD 的辅助治疗
- 批准号:
10735090 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
Evaluation of the Sensitivity to Endocrine Therapy (SET ER/PR) Assay to predict benefit from extended duration of adjuvant endocrine therapy in the NSABP B-42 trial
NSABP B-42 试验中内分泌治疗敏感性 (SET ER/PR) 测定的评估,用于预测延长辅助内分泌治疗持续时间的益处
- 批准号:
10722146 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
AUGMENTING THE QUALITY AND DURATION OF THE IMMUNE RESPONSE WITH A NOVEL TLR2 AGONIST-ALUMINUM COMBINATION ADJUVANT
使用新型 TLR2 激动剂-铝组合佐剂增强免疫反应的质量和持续时间
- 批准号:
10933287 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
DEVELOPMENT OF SAS A SYNTHETIC AS01-LIKE ADJUVANT SYSTEM FOR INFLUENZA VACCINES
流感疫苗类 AS01 合成佐剂系统 SAS 的开发
- 批准号:
10935776 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
DEVELOPMENT OF SMALL-MOLECULE DUAL ADJUVANT SYSTEM FOR INFLUENZA VIRUS VACCINE
流感病毒疫苗小分子双佐剂体系的研制
- 批准号:
10935796 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
A GLYCOLIPID ADJUVANT 7DW8-5 FOR MALARIA VACCINES
用于疟疾疫苗的糖脂佐剂 7DW8-5
- 批准号:
10935775 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
Adjuvant Photodynamic Therapy to Reduce Bacterial Bioburden in High-Energy Contaminated Open Fractures
辅助光动力疗法可减少高能污染开放性骨折中的细菌生物负载
- 批准号:
10735964 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别:
Adjuvant strategies for universal and multiseasonal influenza vaccine candidates in the context of pre-existing immunity
在已有免疫力的情况下通用和多季节流感候选疫苗的辅助策略
- 批准号:
10649041 - 财政年份:2023
- 资助金额:
$ 13.87万 - 项目类别: