Physiology Core

生理学核心

• 批准号: 10747627
• 负责人: Marcelo Daniel Carattino
• 金额: $ 28.79万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747627
• 关键词: Address; Animal Model; Applications Grants; Area; Biological Assay; Biological Models; Biological Process; Carrier Proteins; Cell Line; Cells; Chemicals; Collaborations; Communities; Core Facility; Data; Data Analyses; Data Set; Dedications; Development; Disease; Electrophysiology (science); Ensure; Enzymes; Epithelial Cells; Epithelium; Equipment; Experimental Designs; Generations; Genes; Goals; Health; Human; Imaging Device; Imaging technology; Institution; Ion Transport; Ions; Journals; Kidney; Laboratories; Light; Maintenance; Mammals; Measurement; Mediating; Mediator; Membrane; Membrane Potentials; Modeling; Molecular; Mus; Nephrons; Organ; Organoids; Oryctolagus cuniculus; Perfusion; Phenotype; Physiological; Physiological Processes; Physiology; Preparation; Productivity; Protein Analysis; Proteins; Protocols documentation; Publications; Quality Control; Rattus; Reagent; Regulation; Regulatory Pathway; Renal function; Reproducibility; Research; Research Personnel; Resource Development; Resources; Science; Series; Signaling Molecule; Structure; System; Systems Development; Techniques; Technology; Time; Tissues; Training; Transport Process; Tubular formation; Variant; Work; biomedical resource; cell immortalization; hands-on learning; live cell imaging; member; novel; protein expression; quality assurance; sabbatical; sensor; single molecule; solute; tool; variant of unknown significance

Abstract The Physiology Core provides a series of graded technologies and expertise to investigators who wish to address fundamental questions about transport processes that occur in the tubular structures of the nephron and other relevant systems. To gain understanding of biological processes that mediate kidney function in health and disease states, in light of the phenotypic diversity and functional complexity of this organ, the use of strategies that allow specific nephron segments/cells/proteins to be studied in isolation under defined conditions is required. The Core, through its three Subcores, offers expertise and state-of-the-art technologies for studying the function and regulation of transport and other membrane resident proteins with progressive degrees of complexity from single molecules to model systems to native epithelia. The Single Tubule and Microperfusion Subcore provides tools and technologies to study the function and expression of transport proteins and associated regulatory pathways in single nephron segments isolated from small mammals and kidney organoids. The Electrophysiology Subcore provides expertise and a toolbox of electrophysiological approaches to study the function and regulation of transport proteins at the molecular and cellular levels in model systems and native epithelia. The Live-Cell Imaging Subcore offers imaging tools and technologies to assess ion transport, changes in membrane potential, cellular volume, intracellular mediators, and signaling molecules in immortalized cells and native preparations using chemical and genetically encoded fluorescent sensors. The Core will apply a multipronged approach and collaborate with the O’Brien Consortium to share specialized resources and technologies throughout the kidney research community, to lower the barrier to entry for early-stage investigators and researchers new to the kidney field, and to advance kidney research and discovery nationwide.

摘要 生理学核心为研究人员提供了一系列分级技术和专业知识 解决发生在肾单位小管结构中的运输过程的基本问题 以及其他相关制度。了解肾功能调节的生物学过程 健康和疾病状况，鉴于这一器官的表型多样性和功能复杂性，使用 允许分离研究特定肾单位片段/细胞/蛋白质的策略 条件是必需的。核心，通过其三个子核，提供专业知识和最先进的技术 用于研究转运蛋白和其他膜驻留蛋白的功能和调节 从单分子到模型系统再到天然上皮的复杂程度。单管和单管 微灌注亚核心为研究转运的功能和表达提供了工具和技术 从小型哺乳动物分离的单个肾单位节段中的蛋白质及其相关调控通路 肾脏器官。电生理学分核心提供了电生理学的专业知识和工具箱 在分子和细胞水平上研究转运蛋白功能和调控的方法 模型系统和天然上皮细胞。活细胞成像子核心提供成像工具和技术来 评估离子转运、膜电位、细胞体积、细胞内介质和信号转导的变化 使用化学和基因编码荧光的永生化细胞和天然制剂中的分子 传感器。核心将采取多管齐下的方法，并与奥布莱恩财团合作分享 肾脏研究界的专门资源和技术，以降低 早期研究人员和新进入肾脏领域的研究人员的进入，并促进肾脏研究 和全国范围内的发现。