Pre-Clinical Core

临床前核心

• 批准号: 10746570
• 负责人: PAUL W. SANDERS
• 金额: $ 25.2万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10746570
• 关键词: Acceleration; Acute Renal Failure with Renal Papillary Necrosis; Animal Model; Animal Testing; Area; Basic Science; Benchmarking; Biological Assay; Blood Vessels; COVID-19 pandemic; Capital; Cell Line; Cells; Communities; Core Facility; Country; Creatinine; Data; Education; Educational Activities; Environment; Evaluation; Faculty; Feedback; Female; Fostering; Functional disorder; Goals; Human; Human Resources; In Vitro; Individual; Ischemia; Kidney; Kidney Transplantation; Knowledge; Liquid substance; Medicine; Metabolic; Metabolism; Methodology; Methods; Micropuncture; Mission; Nephrons; Operative Surgical Procedures; Oxygen Consumption; Patients; Peer Review; Physiological; Physiology; Preventive; Procedures; Process; Production; Publications; Quality Control; Renal function; Reperfusion Injury; Research; Research Activity; Research Personnel; Research Project Grants; Resources; Rodent; Rodent Model; Sampling; Sepsis; Services; Technical Expertise; Techniques; Technology; Training; Translations; Transplantation; Tubular formation; animal imaging; animal model development; base; career; design; field study; hemodynamics; human disease; human model; in vivo Model; insight; interest; kidney cell; male; meetings; member; mouse model; novel; pre-clinical; preclinical study; renal tubular reabsorption; skills; therapeutic evaluation; tool; web site

Project Summary Small animal models of human disease have provided important insights into renal pathophysiological processes and are an important preclinical resource to test therapeutic and preventive approaches in acute kidney injury (AKI). The specific aims of the Pre-Clinical Core (Core B) are to: Aim 1. Provide the facilities and skills (Animal Models Resource) to study murine models of AKI. Aim 2. Provide unique facilities and requisite skills (Renal Physiology Resource) to determine renal physiological changes in AKI. This Core will specifically share specialized knowledge, tools, technologies and expertise to (i) utilize rodent models of AKI specifically in the setting of ischemia/reperfusion injury, sepsis and kidney transplantation, and (ii) provide expertise and training for studying renal function on the whole kidney and at the single nephron level, including micropuncture techniques and determination of GFR, microanalysis of tubular fluid and tubular reabsorption, renal hemodynamics with assessment of tubuloglomerular feedback, and metabolic assessment of kidney oxygen consumption in rodents. Core B will also provide technical expertise for the isolation of primary tubular and vascular cells from rodents. The mission of Core B is to provide these unique and relevant resources within the O'Brien Kidney Consortium to help investigators overcome barriers to utilization of relevant rodent models for in vivo and in vitro studies (kidney cells and cell lines) to advance understanding of the pathophysiology of AKI. The Core will capitalize on the excellent, established track record of supporting the national kidney research community through provision of state-of-the-art tools to investigate AKI in animal models. Since the inception of the O'Brien Center, Core B has performed more than 20,000 procedures for the community. The hands-on, technically challenging experimental resources, along with the unique expertise of Core B personnel, will integrate into the Consortium to catalyze collaborative activities and advance AKI research for the kidney research community. These resources have been designed to enhance skills and diversity of the workforce by enticing and supporting early-stage investigators as well as established investigators in other fields of study into AKI-related research.

项目摘要 人类疾病的小动物模型为肾脏病理生理过程提供了重要的见解 并且是测试急性肾损伤的治疗和预防方法的重要临床前资源 （阿基）。临床前核心（核心B）的具体目标是： 目标1.提供研究阿基小鼠模型的设施和技能（动物模型资源）。 目标2.提供独特的设施和必要的技能（肾脏生理学资源），以确定肾脏 阿基的生理变化。 该核心将专门分享专门知识、工具、技术和专业知识，以（i）利用啮齿动物 阿基模型，特别是在缺血/再灌注损伤、脓毒症和肾移植的情况下，和（ii） 提供在整个肾脏和单个肾单位水平上研究肾功能的专业知识和培训， 包括微穿刺技术和GFR的测定，肾小管液和肾小管上皮细胞的微量分析， 重吸收、肾血流动力学和肾小球反馈评估以及代谢评估 肾脏耗氧量的变化。核心B还将提供隔离主要 管状和血管细胞。核心B的使命是提供这些独特的相关资源 在奥布莱恩肾脏联盟内，帮助研究人员克服利用相关啮齿动物的障碍， 用于体内和体外研究的模型（肾细胞和细胞系），以促进对 阿基的病理生理学核心将充分利用卓越的，既定的跟踪记录， 国家肾脏研究界通过提供最先进的工具来研究动物阿基 模型自奥布莱恩中心成立以来，核心B已经为 社区动手，技术上具有挑战性的实验资源，沿着独特的专业知识， 核心B人员将融入联盟，以促进合作活动并推进阿基研究 为肾脏研究界服务这些资源的目的是提高技能和多样性， 通过吸引和支持早期调查人员以及其他领域的成熟调查人员， AKI相关的研究。