MoTrPAC: UC Preclinical Animal Study Site - Supplement

MoTrPAC：UC 临床前动物研究网站 - 补充材料

• 批准号: 10746582
• 负责人: Sue C Bodine
• 金额: $ 35.66万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-15 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10746582
• 关键词: Achievement; Acute; Aerobic Exercise; Age; Animal Experiments; Animal Model; Animals; Body Composition; California; Chemicals; Chronic; Chronic Disease; Clinical Trials; Collection; Complement; Data Analyses; Development; Discipline; Disease; Dose; Environment; Epigenetic Process; Evolution; Exercise; Exercise Physiology; Exercise Test; Funding; Future; Genetic; Goals; Health; Health Benefit; Human; Indirect Calorimetry; Individual; Institution; Insulin; Knowledge; Leadership; Longevity; Maintenance; Maps; Measurement; Metabolic; Metabolism; Modeling; Modification; Molecular; Molecular Target; Morbidity - disease rate; Muscle; Muscle function; Obesity; Organ; Pathway interactions; Phase; Phenotype; Physical Fitness; Physical activity; Physiological; Physiological Adaptation; Proteomics; Protocols documentation; Quality of life; Rattus; Research Personnel; Site; Technology; Testing; Time; Tissue Sample; Tissues; Training; Transducers; Universities; active lifestyle; adverse outcome; animal data; animal imaging; animal model development; blood glucose regulation; body system; cognitive function; endurance exercise; exercise capacity; exercise intervention; exercise program; exercise training; experience; genetic technology; global health; heart function; human model; improved; member; metabolomics; model development; mortality risk; novel; physical inactivity; pre-clinical; prevent; resistance exercise; response; skills; success; trait; transcriptomics

PROJECT SUMMARY/ABSTRACT The University of California Preclinical Animal Study Site (UC PASS) is a collaborative effort between UC Davis and UC San Diego that brings together the scientific expertise, state of the art technologies and facilities, and institutional support to achieve the primary objectives goals of the PASS, which are to: 1) develop animal models that best complement the endurance and resistance training protocols used in the human trials, and 2) provide organ and tissue samples collected at multiple time points after both acute and chronic exercise for analysis by the Chemical Analysis Sites. The animal studies to be undertaken by the PASS will begin to elucidate the molecular pathways responsible for the acute and chronic effects of both endurance and resistance exercise on individual organs, as well as identify the interactions between organs; the goal being to understand the mechanisms by which different modes of physical activity impart their health benefits. In order to reach this goal, experts in model development and phenotyping of the resulting muscle and global health adaptations will be essential. The UC PASS team has exactly these traits. The team acknowledges that the ultimate animal model and exercise tests to be utilized in the PASS will be determined by the Molecular Transducers of Physical Activity Common (MoTrPAC) Steering Committee. In this application we provide a strong rationale and justification for the use of the rat as the animal model and propose specific endurance and resistance exercise programs to be used in Phase I of the PASS, highlighting the major advantages and limitations of each paradigm. In Phase II of the PASS we build on the models established in Phase I and propose three specific aims to begin to produce a standard curve of molecular transducers versus adaptive response to exercise. In Phase II we propose to exercise animals of increasing age or increasing adiposity, determine the adaptive response to 12 weeks of training, and relate this with the acute molecular transducer response to exercise. With this secondary screen, we will be able to identify molecular transducers that directly correlate with the local or global health benefits of exercise. We will then use this information to test the genetic requirement for these molecular transducers for the health benefits of exercise. The UC PASS is composed of an exceptional team of primary investigators and key collaborators/advisors with expertise in the development of animal models of exercise, the molecular response to exercise, and applied human exercise interventions. This team will provide leadership and the necessary skill sets to successfully model human exercise in animals, which is essential to the success of the MoTrPAC Common Fund and achievement of its goal: to define and understand the cellular and molecular mechanisms of the human health benefits of physical activity.

项目摘要/摘要 加利福尼亚大学临床前动物研究网站（UC Pass）是UC之间的合作努力 戴维斯（Davis）和加州大学圣地亚哥（UC San Diego）汇集了科学专业知识，最先进的技术和设施， 和机构支持以实现通行证的主要目标目标，这是：1）发展动物 最能补充人类试验中使用的耐力和抵抗训练方案的模型，以及2） 提供急性和慢性运动后多个时间点收集的器官和组织样品 化学分析位点的分析。通行证要进行的动物研究将开始 阐明负责耐力的急性和慢性作用的分子途径 对个别器官的抵抗运动，并确定器官之间的相互作用；目标是 了解不同的体育活动方式赋予其健康益处的机制。为了 为了实现这一目标，在产生的肌肉和全球健康的模型开发和表型方面的专家 适应至关重要。 UC通行证团队完全具有这些特征。团队承认 最终的动物模型和在通过中使用的运动测试将由分子确定 体育活动的传感器共同（MOTRPAC）指导委员会。在此应用程序中，我们提供了 强烈的理由和使用大鼠作为动物模型的理由，并提出特定的耐力和 抵抗运动计划将在通行证的第一阶段中使用，强调了主要优势和 每个范式的局限性。在通行证的第二阶段中，我们建立在第一阶段和 提出三个特定的目的，以开始产生分子传感器与自适应的标准曲线 对运动的反应。在第二阶段，我们建议行使年龄增加或肥胖增加的动物， 确定对12周训练的自适应反应，并将其与急性分子传感器相关联 对运动的反应。使用此二级屏幕，我们将能够识别直接的分子传感器 与锻炼的本地或全球健康益处相关。然后，我们将使用此信息测试遗传 这些分子换能器对运动的健康益处的要求。 UC通行证由 一支由主要研究人员和主要合作者/顾问组成的杰出团队，具有专业知识 动物模型的运动，分子对运动的反应以及应用人类运动干预措施。 该团队将提供领导和必要的技能，以成功地模拟人类的锻炼 动物，这对于Motrpac共同基金的成功及其目标的成功至关重要： 定义并了解体育活动的人类健康益处的细胞和分子机制。