The Role of Persistent Activity in Visual Working Memory

持续活动在视觉工作记忆中的作用

• 批准号: 10750059
• 负责人: Rye Jaffe
• 金额: $ 4.77万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10750059
• 关键词: Affect; Alzheimer' s Disease; Animals; Area; Behavioral; Brain; Cells; Chronic; Conscious; Cues; Development; Disease; Electric Stimulation; Electrodes; Etiology; Functional Magnetic Resonance Imaging; Human; Implanted Electrodes; Intervention; Judgment; Lateral; Location; Macaca; Macaca mulatta; Maintenance; Manuals; Measures; Mediating; Mediation; Memory; Mental disorders; Metabolic; Microelectrodes; Mind; Modeling; Monkeys; Neurons; Play; Population; Prefrontal Cortex; Reporting; Research; Research Personnel; Role; Short-Term Memory; Site; Stimulus; Stroke; Surface; Synapses; Testing; Time; Training; Traumatic Brain Injury; Visual; cost; experimental study; insight; member; nervous system disorder; neural; neural correlate; neurophysiology; nonhuman primate; novel; novel therapeutics; response; sensory cortex; simulation; visual performance; visual stimulus

PROJECT SUMMARY The proposed study will investigate the role of persistent activity of the lateral prefrontal cortex in object working memory using a non-human primate model. Persistent activity of the prefrontal cortex has long been speculated as the neural correlate of working memory. In recent years alternative models have been proposed, particularly for object working memory. We hypothesize that persistent activity plays a causal role in reflecting the features of remembered stimuli, rather merely representing parameters of the task that subjects perform or highlighting the spatial location of remembered objects. Rhesus macaques will thus be trained in a feature working memory task that will require them to remember and make judgments about visual stimuli. We will record neural activity with a chronic array of microelectrodes, distributed across the surface of the lateral prefrontal cortex, both before and after training. We will test whether the peak of population activity can explain what stimulus the monkeys ultimately remember, and whether drift of this peak of activity over time predicts errors. We will additionally determine how neuronal responses are affected by training by using familiar and novel stimuli in the context of the same working memory task. Moreover, we will directly test the causality of this persistent activity by applying microsimulation on carefully selected electrodes, to cause the animals to erroneously recall not the actual stimulus presented, but the preferred stimulus of the site being stimulated. As a result, the proposed experiments will determine the specific, causal mechanisms in the prefrontal cortex that are ultimately responsible for mediating visual working memory.

项目摘要 本研究旨在探讨外侧前额叶皮层的持续性活动在客体加工中的作用 使用非人类灵长类动物模型的记忆。长期以来，人们一直推测前额皮质的持续活动 作为工作记忆的神经相关物。近年来，已经提出了替代模型，特别是 对象工作记忆。我们假设持续的活动在反映这些特征方面起着因果作用 记忆的刺激，而不仅仅是代表参数的任务，受试者执行或突出显示 记忆物体的空间位置。因此，恒河猴将接受特征工作记忆的训练 这项任务要求他们记住并对视觉刺激做出判断。我们会记录神经活动 与慢性微电极阵列，分布在外侧前额叶皮层的表面，无论是之前， 在训练之后。我们将测试种群活动的峰值是否能解释猴子受到什么刺激 最终记住，以及活动峰值随时间的漂移是否预示着错误。我们还将 在以下背景下使用熟悉和新颖的刺激来确定训练如何影响神经元反应 相同的工作记忆任务。此外，我们将直接测试这种持续活动的因果关系， 在精心选择的电极上进行微模拟，使动物错误地回忆起实际的记忆， 刺激呈现，但被刺激的部位的优选刺激。因此，所提出的实验 将决定特定的，因果机制，在前额叶皮层，最终负责 调节视觉工作记忆。