Label-free cell viability assays using Phase Imaging with Computational Specificity
使用具有计算特异性的相位成像进行无标记细胞活力测定
基本信息
- 批准号:10620330
- 负责人:
- 金额:$ 64.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-10 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAgitationAgreementBindingBiological AssayBioreactorsBusinessesCell CountCell Culture TechniquesCell CycleCell LineCell SurvivalCellsCellular AssayChemicalsClassificationComputer softwareConsumptionDNADataDetectionDevelopmentDrynessEnsureEscherichia coliFeasibility StudiesFluorescenceFundingGeometryGoalsGrowthHourImageImaging DeviceIncubatedInvestmentsLabelLearningLegal patentLettersMammalian CellMarketingMeasurementMechanicsMembraneMicroscopeMicroscopyModalityModelingMonitorMorphologyOptical InstrumentOpticsOutputPerformancePharmacologic SubstancePhasePhotobleachingPhototoxicityPichiaPopulationProceduresProcessProductionPumpReagentRecombinant ProteinsResearchResearch Project GrantsRouteSaccharomycesSalesSamplingSmall Business Innovation Research GrantSoftware ToolsSpecialistSpecificitySpecimenStainsStimulusStressStructureSuspensionsTechnologyTestingTimeTrainingTrypan BlueTrypsinWorkartificial intelligence algorithmcell growthcell injurycellular imagingcommercial prototypecommercializationdeep learningdeep learning algorithmdeep neural networkdesigndigitalexperiencefeedingfluorescence imagingfluorophorehigh riskimaging modalityimaging systeminstrumentinterestmanufacturemonolayernoveloperationprototypequality assurancequantitative imagingresearch and developmentsoftware developmenttomography
项目摘要
Project Summary/Abstract
Viable cell concentration is one of the most important key performance indicator during upstream technologies
in mammalian cell culture. Customer Discovery discussions with biopharma execs and R&D specialists revealed
there is an unmet need for on-line/in-line monitoring of cell viability in pharma bioreactors driven by compliance
with real-time product quality assurance as described in Process Analytical Technology (PAT) and Quality by
Design initiatives.
Preliminary work equivalent to Phase I effort has resulted in a cell imaging system including a flow cell setup that
simulates an on-line stirring bioreactor sampling route. Proof-of-concept was also established for detection and
classification of live/dead assays using digital staining via PICS (Phase Imaging with Computational Specificity).
This Small Business Innovation Research project proposes to build a commercial prototype instrument for real
time, quantitative and high-throughput cell viability scoring for on-line/in-line bioreactor monitoring that meets the
metrics of interest for pharma R&D applications. During this effort the standard operation of the QPI+PICS
instrument will be refined and the automated software detection and classification of live/dead assays will be
completed.
At the end of this Direct Phase II project we will deliver a commercial prototype -a “push-of-a-button” viability
scanner with the above capabilities built in.
项目总结/摘要
活细胞浓度是上游技术中最重要的关键性能指标之一
在哺乳动物细胞培养中。与生物制药高管和研发专家的客户发现讨论显示,
存在对药物生物反应器中的细胞活力的在线/在线监测的未满足的需求
实时产品质量保证,如过程分析技术(PAT)和质量
设计倡议。
与第一阶段工作相当的初步工作已经产生了包括流动池装置的细胞成像系统,
模拟在线搅拌生物反应器采样路线。还建立了用于检测的概念验证,
通过PICS(具有计算特异性的相位成像)使用数字染色对活/死测定进行分类。
这个小企业创新研究项目提出建立一个商业原型仪器,用于真实的
用于在线/在线生物反应器监测的时间、定量和高通量细胞活力评分,
制药研发应用的重要指标。在此过程中,QPI+PICS的标准操作
仪器将得到完善,自动软件检测和分类的活/死化验将
完成
在这个直接第二阶段的项目结束时,我们将提供一个商业原型-一个“按下一个按钮”的可行性
扫描仪内置上述功能。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
CATALIN CHIRITESCU其他文献
CATALIN CHIRITESCU的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('CATALIN CHIRITESCU', 18)}}的其他基金
Label-free cell cycle classification using Phase Imaging with Computational Specificity
使用具有计算特异性的相位成像进行无标记细胞周期分类
- 批准号:
10547446 - 财政年份:2022
- 资助金额:
$ 64.99万 - 项目类别:
Label-free cell viability assays using Phase Imaging with Computational Specificity
使用具有计算特异性的相位成像进行无标记细胞活力测定
- 批准号:
10484781 - 财政年份:2022
- 资助金额:
$ 64.99万 - 项目类别:
相似海外基金
Relationship between Biomarkers of Oxidative Stress and Agitation Severity in Moderate-to-severe Alzheimer's Disease
中重度阿尔茨海默病氧化应激生物标志物与躁动严重程度之间的关系
- 批准号:
497994 - 财政年份:2023
- 资助金额:
$ 64.99万 - 项目类别:
Effects of dexmedetomidine on agitation in critically ill TBI patients - DEX-TBI
右美托咪定对危重 TBI 患者躁动的影响 - DEX-TBI
- 批准号:
488402 - 财政年份:2023
- 资助金额:
$ 64.99万 - 项目类别:
Operating Grants
Co-design and evaluation of sensor-instrumented ‘smart socks’ (MPATIX) to improve management of distress and agitation for people with dementia
共同设计和评估传感器仪表“智能袜子”(MPATIX),以改善痴呆症患者的痛苦和躁动管理
- 批准号:
10055596 - 财政年份:2023
- 资助金额:
$ 64.99万 - 项目类别:
Collaborative R&D
Identifying pre-agitation biometric signature in dementia patients: A feasibility study
识别痴呆症患者的躁动前生物识别特征:可行性研究
- 批准号:
486612 - 财政年份:2022
- 资助金额:
$ 64.99万 - 项目类别:
Studentship Programs
Relationship between 4-Hydroxynonenal and Agitation Severity in Alzheimer’s Disease
4-羟基壬烯醛与阿尔茨海默病患者躁动严重程度之间的关系
- 批准号:
486589 - 财政年份:2022
- 资助金额:
$ 64.99万 - 项目类别:
Studentship Programs
Agitation in Alzheimer's Disease: Identification and Prediction Using Digital Behavioral Markers and Indoor Environmental Factors
阿尔茨海默病中的躁动:使用数字行为标记和室内环境因素进行识别和预测
- 批准号:
10404523 - 财政年份:2021
- 资助金额:
$ 64.99万 - 项目类别:
Clinical Decision Support Tool to Assess Risk and Prevent Agitation Events
用于评估风险和预防躁动事件的临床决策支持工具
- 批准号:
10683499 - 财政年份:2021
- 资助金额:
$ 64.99万 - 项目类别:
Development of Memesto, a wearable repetitive message and music therapy device that senses and reduces agitation in persons with AD/ADRD.
开发 Memesto,一种可穿戴式重复信息和音乐治疗设备,可感知并减少 AD/ADRD 患者的躁动。
- 批准号:
10322846 - 财政年份:2021
- 资助金额:
$ 64.99万 - 项目类别:
Agitation in Alzheimer's Disease: Identification and Prediction Using Digital Behavioral Markers and Indoor Environmental Factors
阿尔茨海默病中的躁动:使用数字行为标记和室内环境因素进行识别和预测
- 批准号:
10190522 - 财政年份:2021
- 资助金额:
$ 64.99万 - 项目类别:
Clinical Decision Support Tool to Assess Risk and Prevent Agitation Events
用于评估风险和预防躁动事件的临床决策支持工具
- 批准号:
10365272 - 财政年份:2021
- 资助金额:
$ 64.99万 - 项目类别: