Molecular Immunology Core

核心摘要/摘要 MIC将为所有生物的高级细胞和系统免疫分析提供一个集中的中心 来自项目1-2的样本。此外，MIC将协调和简化所有常见的分析和 确保两个项目都采用统一的标准作业程序和最佳做法的程序。 MIC由五个相互作用的子核组成：(1)MHC免疫遗传学和分型核心；(2)Flow 细胞学和细胞因子核心；(3)病毒学和功能保护性免疫核心；(4)分子免疫学 和翻译核心；以及(5)计算分析核心。每个核心都满足一项基本要求 以最佳方式进行项目1-2中提出的复杂实验，并促进协作 调查人员之间。因此，MIC将显著提高我们小组的效率和严谨性 能够执行本U19中提出的肺移植耐受实验。

CORE SUMMARY / ABSTRACT The MIC will provide a centralized hub for advanced cellular and systems immunological analysis of all biologic samples derived from Projects 1-2. In addition, the MIC will coordinate and streamline all common assays and procedures to ensure that both Projects utilize uniform Standard Operating Procedures (SOPs) and best practices. The MIC is comprised of five interacting sub-Cores: (1) The MHC Immunogenetics and Typing Core; (2) the Flow Cytometry and CyTOF Core; (3) The Virology and Functional Protective Immunity Core; (4) The Molecular Immunology and Transcriptomics Core; and (5) The Computational Analysis Core. Each Core addresses an essential requirement for the optimal conduct of the complex experiments that are proposed in Projects 1-2 and promotes collaboration between investigators. As such, the MIC will significantly increase the efficiency and the rigor with which our group is able to execute the lung transplant tolerance experiments proposed in this U19.