Molecular Immunology Core

分子免疫学核心

• 批准号: 10622125
• 负责人: Leslie S Kean
• 金额: $ 54.76万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-23 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10622125
• 关键词: Address; Allograft Tolerance; Animals; Automobile Driving; Bioinformatics; Biological; Biological Assay; Bone Marrow; Boston; Cells; Clinic; Clone Cells; Collaborations; Complex; Computer Analysis; Computer software; Cytomegalovirus; Dana-Farber Cancer Institute; Data Set; Development; Disparity; Ensure; Flow Cytometry; General Hospitals; Graft Rejection; Hospitals; Human Herpesvirus 4; Immune; Immune Tolerance; Immune response; Immune system; Immunity; Immunogenetics; Immunologic Monitoring; Immunology; Immunology procedure; Information Management; Lead; Link; Lung; Lung Transplantation; Macaca; Macaca fascicularis; Macaca mulatta; Massachusetts; Methodology; Minnesota; Molecular; Molecular Analysis; Molecular Immunology; Monitor; Outcome; Pathway interactions; Pediatric Hospitals; Performance; Physicians; Procedures; Regimen; Reproducibility; Research; Research Personnel; Safety; Sampling; Scientist; Stains; Standardization; System; Systems Analysis; T cell infiltration; T-Lymphocyte; Techniques; Translating; Transplant Recipients; Transplantation; Transplantation Tolerance; Universities; Viral; Virus; Virus Latency; Woman; bioinformatics pipeline; cell type; clinically relevant; data management; experimental study; high dimensionality; lung allograft; medical schools; novel; novel strategies; novel therapeutic intervention; single-cell RNA sequencing; spatiotemporal; synergism; tool; transcriptomics; viral detection; virology

CORE SUMMARY / ABSTRACT The MIC will provide a centralized hub for advanced cellular and systems immunological analysis of all biologic samples derived from Projects 1-2. In addition, the MIC will coordinate and streamline all common assays and procedures to ensure that both Projects utilize uniform Standard Operating Procedures (SOPs) and best practices. The MIC is comprised of five interacting sub-Cores: (1) The MHC Immunogenetics and Typing Core; (2) the Flow Cytometry and CyTOF Core; (3) The Virology and Functional Protective Immunity Core; (4) The Molecular Immunology and Transcriptomics Core; and (5) The Computational Analysis Core. Each Core addresses an essential requirement for the optimal conduct of the complex experiments that are proposed in Projects 1-2 and promotes collaboration between investigators. As such, the MIC will significantly increase the efficiency and the rigor with which our group is able to execute the lung transplant tolerance experiments proposed in this U19.

核心摘要/摘要 MIC将为所有生物制品的先进细胞和系统免疫学分析提供一个集中的中心。 样本来自项目1-2。此外，MIC将协调和简化所有常见的测定， 确保两个项目采用统一的标准操作程序（SOP）和最佳实践的程序。 MIC由五个相互作用的子核心组成：（1）MHC免疫遗传学和分型核心;（2）流动核心;（3）免疫学和免疫学核心。 细胞计数和CyTOF核心;（3）病毒学和功能保护性免疫核心;（4）分子免疫学 和转录组学核心;和（5）计算分析核心。每个核心都满足一个基本要求 项目1-2中提出的复杂实验的最佳实施，并促进合作 调查员之间。因此，MIC将大大提高我们小组的效率和严谨性， 能够执行本U19中提出的肺移植耐受性实验。