Eradication of clonally expanded CD4+ T cells

项目摘要 克隆扩增是一个过程，其中潜伏感染的CD4+ T细胞增殖， 对其同源抗原的反应。据认为，这一过程对 艾滋病毒储存库的大小，因此破坏这种机制可能会产生一种 对潜伏感染细胞的数量有巨大的影响。我们观察到 在一个精英抑制者中， 我们假设我们可以在体外重现这一点， 通过用同源抗原的组合刺激CD4+ T细胞以诱导 增殖，以及化疗或抗增殖剂， 选择性杀死分裂的抗原特异性CD4+ T细胞。如果成功，两步 用同源抗原对个体进行疫苗接种和短期治疗的过程 化疗剂或抗增殖剂可以是减少肿瘤大小的策略的一部分， 的水库。

Project Summary Clonal expansion is a process where latently infected CD4+ T cells proliferate in response to their cognate antigen. It is thought that this process contributes significantly to the size of the HIV reservoir and therefore disrupting this mechanism could have a huge impact on the number of latently infected cells. We observed a significant decline in the number of expanded CD4+ T cell clones in an elite suppressor who received chemoradiation for lung cancer and we hypothesize that we can recapitulate this in vitro by stimulating CD4+ T cells with a combination of cognate antigen to induce proliferation, and either chemotherapeutic or antiproliferative agents that would selectively kill the dividing antigen-specific CD4+ T cells. If successful, a two-step process of vaccination of individuals with cognate antigen and short term treatment with chemotherapeutic or antiproliferative agents may be part of a strategy to reduce the size of the reservoir.