ShEEP Request for Particle Matrix ZetaView

ShEEP 请求粒子矩阵 ZetaView

• 批准号: 10741098
• 负责人: Lynn PULLIAM
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10741098
• 关键词: 8 year old; Affect; Amyotrophic Lateral Sclerosis; Antibodies; Area; Biological; Biological Markers; Carbon Nanotubes; Cardiovascular Diseases; Charge; Communities; Computer software; Diabetes Mellitus; Disease; Dyes; Equipment; Europe; Explosion; Fluorescence; Frontotemporal Dementia; Funding; Future; Goals; HIV; Health; Homeostasis; Inflammation; Label; Lasers; Lighting; Lipids; Liposomes; Malignant Neoplasms; Measures; Methods; Nerve Degeneration; Particle Size; Pathologic; Performance; Polymers; Process; Research; Sampling; San Francisco; Sheep; Surface; Suspensions; System; Techniques; Technology; Thinking; Time; Vesicle; Veterans; Virus; exosome; extracellular vesicles; instrument; nanoparticle; new technology; particle; programs; protein aggregation; user-friendly

Extracellular vesicle (EV) research is a fast-moving area of research. Biological nanoparticles such as EVs, exosomes, viruses, lipids are part of normal homeostasis and now we believe a major part of pathological conditions. The San Francisco VA was the first in the UCSF community to purchase a NanoSight nanoparticle tracking system, 8 years ago. The field has moved very quickly and the new Particle Matrix ZetaView, we believe has replaced the NanoSight technology. The ZetaView can rapidly characterize particles as to size, particle concentration and surface charge. It also has the advantage of characterizing particles by fluorescent labelling without any additives and with a very small sample. The ZetaView has 4 lasers to enable the user to use different dyes to discriminate different particles and allows the user to quantify colocalization ratios of biomarkers. This nanoparticle tracking analysis is rapid and user friendly. This basic characterization of EV particles is essential in any project to use EVs as biomarkers and treatments. This new machine will attract new users to the field of EVS and facilitate current users for their funded projects.

细胞外小泡(EV)的研究是一个快速发展的研究领域。生物纳米颗粒 如EVS、外切体、病毒、脂质是正常内稳态的一部分，现在我们认为 病理情况的主要部分。旧金山退伍军人管理局是加州大学旧金山分校社区中第一个 购买NanoSight纳米颗粒跟踪系统，8年前。这一领域已经发生了很大的变化 快速和新的粒子矩阵ZetaView，我们相信已经取代了NanoSight技术。 ZetaView可以快速表征颗粒的大小、浓度和表面 充电。它还具有通过荧光标记来表征颗粒的优点，而不需要任何 添加剂和非常小的样品。ZetaView有4个激光器，用户可以使用 不同的染料可以区分不同的颗粒，并允许用户量化共存比率 生物标记物。这种纳米颗粒跟踪分析是快速和用户友好的。这是基本的 电动汽车颗粒的特性在任何将电动汽车用作生物标志物和 治疗。这台新机器将吸引新用户进入电动汽车领域，并为现有用户提供便利 用于他们资助的项目。