Human tissue collection, processing and repository Core

人体组织采集、处理和储存核心

• 批准号: 10592410
• 负责人: Matthew F. Tector
• 金额: $ 32.22万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10592410
• 关键词: Age; Alabama; Aliquot; Antigens; B-Lymphocytes; Bacterial Antigens; Bar Codes; Biology; Blood; Blood specimen; Brain Death; Cell Separation; Cell Survival; Cells; Cellular biology; Clinical Data; Clinical Research; Clinical Trials; Collaborations; Collection; Communities; Cryopreservation; Data; Databases; Dissociation; Eligibility Determination; Ensure; Equipment and supply inventories; Evaluation; Flow Cytometry; Flushing; Freezing; Goals; HIV; Haplotypes; Harvest; Health Insurance Portability and Accountability Act; Heart Transplantation; Hepatitis B Virus; Hepatitis C virus; Histocompatibility Antigens; Hospitals; Human; Humoral Immunities; Immunity; Immunoglobulin-Secreting Cells; Immunologics; Individual; Informatics; Infrastructure; Investigation; Ischemia; Kidney Transplantation; Label; Laboratory Research; Leukocytes; Link; Location; Lung Transplantation; Lymphocyte; Methods; Operative Surgical Procedures; Organ; Organ Donations; Organ Donor; Organ Procurements; Organ Transplantation; Organ Weight; Plasma; Procedures; Process; Quality Control; Recovery; Reproducibility; Research; Research Personnel; Research Support; Residual state; Resources; Sampling; Secure; Series; Serum; Solid; Specimen; Standardization; Structure; Suspensions; System; Time; Tissue Banks; Tissue Procurements; Tissue Transplantation; Tissues; Translational Research; Transplantation; Vial device; Viral Antigens; Work; biobank; clinical center; cryogenics; design; experimental study; human tissue; innovation; liver transplantation; novel; prevent; programs; repository; response; searchable database; sex; success

Core B: Human Tissue Collection, Processing, and Repository Core Project Summary Circulating lymphocytes are easy to access and therefore have been intensively studied in human beings. In contrast, much less is known regarding the biology of tissue-resident human lymphocytes. This problem exists because of limited access to sufficient quantities of human tissues. The overarching goal of this U19 Project is to advance understanding of humoral immunity by evaluating B cells in eight different human tissues. Three projects and three scientific cores will collaborate to examine the responses of B cells in these tissues against bacterial, viral, and transplant antigens. Core B's objective is to eliminate the challenges associated with human tissue access in support of the U19 goals. The organizational and operational structure of Core B is innovative because a team with broad expertise (surgical, immunologic, cell biology, and biorepositories) will be aligned with the Alabama Organ Center (AOC). The AOC manages deceased organ donors for the purposes of tissue transplantation and is on the same campus as Core B. This structure will enable the collection of tissues from approximately 19 human donors per year. Further, Core B will isolate, quality control and store leukocytes in a biorepository. The rationale for this approach is that it will not only increase human tissue access, it will standardize the isolation and storage of high-quality leukocytes. The repository component will enable the projects and cores of this U19 to search this collection to find the ideal samples for each experiment. This setup also facilitates the collaborative aspects of this U19 by enabling all projects to understand which samples exist so that sharing can be prioritized to maximize sample value. In Specific Aim 1, we will collect tissues from deceased organ donors via the AOC. The objective of Specific Aim 2 is to isolate and cryopreserve leukocytes from the tissues. Lastly, Specific Aim 3 will establish a searchable biorepository of these samples with which to support the research objectives of the U19. The activities of Core B are significant because they will eliminate barriers that have prevented access to tissue-resident lymphocytes, and they will enable the comparison of antigen-specific B cells in multiple tissues collected from the same individual, as well as comparison to other individuals. Many of these investigations will be entirely novel.

核心B：人体组织采集、处理和储存核心 项目摘要 循环淋巴细胞很容易获得，因此在人类中得到了深入研究。在 相比之下，关于组织驻留的人淋巴细胞的生物学知之甚少。存在这个问题 因为获取足够数量的人体组织的途径有限。U19项目的总体目标 是通过评估八种不同人体组织中的B细胞来增进对体液免疫的理解。三 项目和三个科学核心将合作研究这些组织中的B细胞对 细菌、病毒和移植抗原。核心B的目标是消除与以下方面相关的挑战： 人体组织获取，以支持U19目标。核心B的组织和运营结构是 创新，因为一个拥有广泛专业知识（手术，免疫学，细胞生物学和生物储存库）的团队将 与亚拉巴马器官中心（AOC）保持一致。AOC负责管理已故器官捐赠者， 组织移植的目的，并在同一个校园的核心B。并认为此架构能使本 每年从大约19名人类捐赠者那里收集组织。此外，核心B将进行分离、质量控制 并将白细胞储存在生物储存库中。这种方法的基本原理是，它不仅会增加人类 组织获取，它将标准化高质量白细胞的分离和储存。存储库 组件将使这个U19的项目和核心搜索这个集合，以找到理想的样本， 每个实验。此设置还通过使所有项目能够 了解哪些样本存在，以便可以优先共享，以最大限度地提高样本价值。具体目标 1.我们会透过器官捐赠中心收集已故器官捐赠者的组织。具体目标2的目标是 从组织中分离并冷冻保存白细胞。最后，具体目标3将建立一个搜索 这些样品的生物储存库，以支持U19的研究目标。核心的活动 B很重要，因为它们将消除阻碍获得组织驻留的障碍 淋巴细胞，并且它们将使得能够比较从人淋巴细胞收集的多种组织中的抗原特异性B细胞。 同一个人，以及与其他人的比较。其中许多调查将完全 小说