Characterization of virus-specific human B cell subsets in lymphoid and non-lymphoid tissues

淋巴和非淋巴组织中病毒特异性人类 B 细胞亚群的表征

基本信息

  • 批准号:
    10592418
  • 负责人:
  • 金额:
    $ 94.58万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-04-01 至 2025-03-31
  • 项目状态:
    未结题

项目摘要

Project 2: Characterization of Virus-Specific Human B Cell Subsets in Lymphoid and Non-Lymphoid Tissues Project Summary Antibodies against influenza virus protect against infection and reduce morbidity and mortality. As a result, vaccines against influenza are designed to elicit antibodies against circulating strains of virus. Given that antibodies are made by B cells, it makes sense to characterize influenza-specific B cells in order to determine how they are selected, how they function and where they are maintained. However, most studies of influenza- specific B cells in humans are limited to B cells from peripheral blood, rather than those in lymphoid organs or the lung. Moreover, most studies characterize influenza-specific antibody-secreting cells (ASCs), which circulate for a short period after infection or vaccination. Consequently, we have minimal understanding of influenza-specific memory B cells or ASCs in human tissues. Thus, there is an urgent need to characterize the phenotypes and functions of influenza-specific B cells in human lymphoid and non-lymphoid tissues. Given this gap in knowledge, we are proposing experiments that will use “B cell tetramers” to identify influenza-specific B cells responding to hemagglutinin (HA), nucleoprotein (NP) and non-structural-1 (NS1) proteins of influenza virus in blood, lymphoid tissues, lung and visceral adipose tissues of normal human donors. We will then determine how the phenotypes, transcriptional programs, functional properties, reactivities and affinities of those B cells differ between tissues and between B cells of different specificities. Importantly, we will use an automated, single-cell cloning and antibody-expression system to rapidly and efficiently generate recombinant, influenza-specific antibodies from sorted memory B cells in order to determine their affinity and cross-reactivity. Therefore, we believe that this project is highly innovative and will significantly advance our understanding of influenza-specific B cell biology in various human tissues.
项目2:淋巴和非淋巴病毒特异性人B细胞亚群的表征

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Frances E. Lund其他文献

Transcription factor T-bet regulates the maintenance and differentiation potential of lymph node and lung effector memory B cell subsets
转录因子 T 细胞结合抑制因子调节淋巴结和肺效应记忆 B 细胞亚群的维持和分化潜能
  • DOI:
    10.1016/j.immuni.2025.05.021
  • 发表时间:
    2025-07-08
  • 期刊:
  • 影响因子:
    26.300
  • 作者:
    Christopher A. Risley;Michael D. Schultz;S. Rameeza Allie;Shanrun Liu;Jessica N. Peel;Anoma Nellore;Christopher F. Fucile;Christopher D. Scharer;Jeremy M. Boss;Troy D. Randall;Alexander F. Rosenberg;Frances E. Lund
  • 通讯作者:
    Frances E. Lund
IgM Memory Cells: First Responders in Malaria
  • DOI:
    10.1016/j.immuni.2016.08.005
  • 发表时间:
    2016-08-16
  • 期刊:
  • 影响因子:
  • 作者:
    Sara L. Stone;Frances E. Lund
  • 通讯作者:
    Frances E. Lund
This information is current as Infection in Mice Pneumocystis Clearance of T Cells for + Early Priming of CD 4 B Lymphocytes Are Required during the Feola
此信息是最新的,因为小鼠肺孢子虫感染在 Feola 期间需要清除 T 细胞以早期启动 CD 4 B 淋巴细胞
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M. Opata;M. Hollifield;Frances E. Lund;Troy D. Randall;Robert Dunn;B. Garvy;D. Feola
  • 通讯作者:
    D. Feola
Signaling through CD38 augments B cell antigen receptor (BCR) responses and is dependent on BCR expression.
通过 CD38 的信号传导可增强 B 细胞抗原受体 (BCR) 反应,并且依赖于 BCR 表达。
  • DOI:
    10.4049/jimmunol.157.4.1455
  • 发表时间:
    1996
  • 期刊:
  • 影响因子:
    4.4
  • 作者:
    Frances E. Lund;N.;K. M. Kim;M. Reth;Maureen Howard
  • 通讯作者:
    Maureen Howard
This information is current as MechanismsStrains of Influenza via Multiple B Cells Promote Resistance to Heterosubtypic and
该信息是最新的,因为机制流感菌株通过多个 B 细胞促进对异亚型和
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J. Rangel;D. Carragher;Ravi S. Misra;Kim L. Kusser;Louise Hartson;A. Moquin;Frances E. Lund;T. Randall
  • 通讯作者:
    T. Randall

Frances E. Lund的其他文献

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{{ truncateString('Frances E. Lund', 18)}}的其他基金

TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10642784
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10431929
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
Tissue and organ specific human B cell immunity
组织和器官特异性人类 B 细胞免疫
  • 批准号:
    10265689
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10032785
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
Identification and characterization of effector memory B cell populations that dominate memory responses to subsequent influenza infection and vaccination
效应记忆 B 细胞群的鉴定和表征,该细胞群主导对随后流感感染和疫苗接种的记忆反应
  • 批准号:
    10227903
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
Identification and characterization of effector memory B cell populations that dominate memory responses to subsequent influenza infection and vaccination
效应记忆 B 细胞群的鉴定和表征,该细胞群主导对随后流感感染和疫苗接种的记忆反应
  • 批准号:
    10455632
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10214491
  • 财政年份:
    2020
  • 资助金额:
    $ 94.58万
  • 项目类别:
Administrative Core
行政核心
  • 批准号:
    10395996
  • 财政年份:
    2019
  • 资助金额:
    $ 94.58万
  • 项目类别:
Tissue and organ specific human B cell immunity
组织和器官特异性人类 B 细胞免疫
  • 批准号:
    10592408
  • 财政年份:
    2019
  • 资助金额:
    $ 94.58万
  • 项目类别:
Administrative Core
行政核心
  • 批准号:
    10592409
  • 财政年份:
    2019
  • 资助金额:
    $ 94.58万
  • 项目类别:

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ADVANCE 合作伙伴关系:利用工业工程和运筹学 (LINEAGE) 领域的交叉性和工程亲和力团体
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