SARS CoV2 Studies in the Helminth Immunology Section/LPD

为了应对SARS-CoV-2大流行，我们利用我们在人类T细胞克隆方面的专业知识，从未感染（SARS-CoV 2-naive）个体的幼稚CD 4+和CD 8 + T细胞中产生SARS-CoV 2-specificT细胞系。为此，我们产生了由定义的综合肽库驱动的S特异性、M特异性、CMV特异性（对照）、CEF特异性和0 c43特异性人TCL（来自6个个体的总共96个系）。我们的数据清楚地表明，大多数CD 4 + SARS CoV 2特异性细胞具有效应记忆表型。更重要的是，对S特异性和M特异性人TCL的scRNAseq分析表明，刺突蛋白驱动有利于抗病毒活性的1型和III型干扰素途径，而M特异性人T细胞抑制有利于病毒复制的这些（和其他途径）。

In response to the SARS-CoV-2 pandemic, we leveraged our expertise in human T cell cloning to generate SARS-CoV2-specificT cell lines from naive CD4+ and CD8+ T cells of uninfected (SARS Cov2-naive) individuals. To this end, we generated S-specific, M-specific, CMV-specific (controls), CEF-specific and Oc43-specific human TCLs (a total of 96 lines from 6 individuals) driven by defined comprehensive peptide pools. Our data demonstrate clearly that the majority of the CD4+ SARS CoV2-specific cells have an effector memory phenotype. More importantly, scRNAseq analysis on the S-specific and the M-specific human TCLs indicates that the Spike protein drives Type 1 and Type III interferon pathways that favor anti-viral activity whereas the M-specific human T cells suppresses these (and other pathways) that favor viral replication.