ORGANOCHLORINE COMPOUNDS AND MENSTRUAL CYCLE FUNCTION

有机氯化合物与月经周期功能

• 批准号: 2518710
• 负责人: GAYLE C WINDHAM
• 金额: $ 11.55万
• 依托单位: PUBLIC HEALTH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2518710
• 关键词: biomarker; chlordane; chlorohydrocarbon insecticide; clinical research; dichlorodiphenyltrichloroethane; environmental contamination; epidemiology; estrogen analog; female; food contamination; gas chromatography; gas chromatography mass spectrometry; hexachlorobenzene; hormone regulation /control mechanism; human subject; lifestyle; menstrual cycle; nutrition related tag; pesticide biological effect; pesticide residues; racial /ethnic difference

The purpose of this proposal is to examine whether women who have been exposed to chlorinated pesticides, which have been identified as endocrine disruptors in animals, have alterations in their ovarian function as measured by frequency of menstrual cycle disturbances. Such disturbances may impact the frequency of infertility and sub-fertility, as well as that of other hormone-dependent illnesses such as breast cancer and osteoporosis, which affect large numbers of women. The target exposed population is a group of Laotian immigrants who are frequent consumers of fish caught locally in the San Francisco Bay. Contamination levels above health protective advisories have been documented in Bay fish and shellfish. Immigration from Asia, as well as consumption of contaminated fish, have been shown to be associated with higher organochlorine pesticide body burdens. Exposure will be measured by determining levels of DDT and other pesticide metabolites in a serum sample. Menstrual function will be assessed by measuring urinary metabolites of steroid hormones daily during three menstrual cycles of approximately 50 Laotian women. We are currently studying menstrual function in a group of 400 women, so we have developed algorithms to determine ovulatory status, day of ovulation and menstrual cycle disturbances such as short luteal phase, which will also be applied to the new sample. This previously-collected sample will serve as a baseline comparison group, as well as a source of data on the effects of lifestyle factors (such as smoking and alcohol consumption) on hormone function. The availability of this previously assembled cohort allows for more efficient examination of a small group of exposed women. This will be one of the first studies to examine biomarkers of exposure in relation to patterns of the menstrual cycle assessed directly by daily hormone levels. In addition to serum pesticide levels, questionnaire measures of exposure (e.g. species-specific fish consumption) will be examined in relation to ovarian function defined as continuous measures (e.g. cycle length) and dichotomous "abnormalities" (e.g. short luteal phase or anovulation). If interesting associations are revealed, follow-up in larger populations, with a larger gradient of exposure, will be warranted.

这项提议的目的是审查那些曾经被 接触氯化杀虫剂，已被确认为内分泌 在动物身上的干扰物，会改变它们的卵巢功能 以月经周期紊乱的频率来衡量。这样的骚乱 可能会影响不孕和亚生育的频率，以及 其他激素依赖型疾病，如乳腺癌和 骨质疏松症，这影响了大量女性。暴露的目标 人口是一群老挝移民，他们经常消费 在旧金山湾本地捕捞的鱼。污染水平高于 健康保护建议已被记录在海湾鱼和 贝类。来自亚洲的移民，以及受污染的 鱼，已被证明与较高的有机氯有关 杀虫剂的身体负担。将通过确定以下水平来衡量暴露水平 血清样本中的滴滴涕和其他农药代谢物。月经功能 将通过测量尿液中类固醇激素的代谢物来评估 在大约50名老挝妇女的三个月经周期期间，每天都会发生这种情况。我们 目前正在研究400名女性的月经功能，所以我们 已经开发了算法来确定排卵状态、排卵日期 以及月经周期紊乱，如黄体期过短，这将 也适用于新的样品。之前收集的样本将 作为基准比较组，也是关于 生活方式因素(如吸烟和饮酒)对 荷尔蒙功能。这个先前集结的队列的可用性 允许更有效地检查一小部分接触过病毒的妇女。 这将是首批研究暴露于环境的生物标志物之一。 与每日直接评估的月经周期类型的关系 荷尔蒙水平。除了血清农药水平，问卷调查 暴露的衡量标准(例如特定物种的鱼类消费)将是 与卵巢功能相关的检查，定义为连续测量 (例如周期长度)和二分性“异常”(例如黄体短 阶段或无排卵)。如果发现了有趣的关联，后续行动 在更大的人群中，接触的梯度更大，将是 这是正当的。