ORGANOCHLORINE COMPOUNDS AND MENSTRUAL CYCLE FUNCTION

这项建议的目的是审查妇女是否 暴露于氯化农药，这已被确定为内分泌 在动物中的干扰物，有卵巢功能的改变， 以月经周期紊乱的频率来衡量。这样的扰动 可能会影响不孕症和生育能力低下的频率， 其他依赖性疾病，如乳腺癌， 骨质疏松症，影响到大量妇女。暴露的目标 人口是一群老挝移民，他们经常消费 在旧金山弗朗西斯科湾当地捕获的鱼。污染水平高于 在海湾鱼类中已经记录了健康保护性寄生虫， 贝类。来自亚洲的移民，以及消费受污染的 鱼类，已被证明与较高的有机氯 农药的身体负担。将通过测定以下物质的水平来测量暴露： 血清样本中的滴滴涕和其他农药代谢物。月经功能 将通过测量类固醇激素的尿代谢产物进行评估 在大约50名老挝妇女的三个月经周期中每天进行一次。我们 目前正在研究一组400名妇女的月经功能，所以我们 已经开发出算法来确定排卵状态，排卵日 和月经周期紊乱，如黄体期短，这将 也适用于新样品。这份先前采集的样本将 作为一个基线比较组，以及关于 生活方式因素（如吸烟和饮酒）对 激素功能这个先前聚集的队列的可用性 可以更有效地检查一小群暴露的妇女。 这将是第一个研究暴露在环境中的生物标志物的研究。 与月经周期模式的关系， 荷尔蒙水平除了血清农药水平，问卷调查 暴露的测量（例如，特定物种的鱼类消费量）将 与卵巢功能相关的检查定义为连续测量 (e.g.周期长度）和二分“异常”（如黄体短 阶段或无排卵）。如果发现了有趣的关联， 在更大的人群中，有更大的暴露梯度， 有正当理由

The purpose of this proposal is to examine whether women who have been exposed to chlorinated pesticides, which have been identified as endocrine disruptors in animals, have alterations in their ovarian function as measured by frequency of menstrual cycle disturbances. Such disturbances may impact the frequency of infertility and sub-fertility, as well as that of other hormone-dependent illnesses such as breast cancer and osteoporosis, which affect large numbers of women. The target exposed population is a group of Laotian immigrants who are frequent consumers of fish caught locally in the San Francisco Bay. Contamination levels above health protective advisories have been documented in Bay fish and shellfish. Immigration from Asia, as well as consumption of contaminated fish, have been shown to be associated with higher organochlorine pesticide body burdens. Exposure will be measured by determining levels of DDT and other pesticide metabolites in a serum sample. Menstrual function will be assessed by measuring urinary metabolites of steroid hormones daily during three menstrual cycles of approximately 50 Laotian women. We are currently studying menstrual function in a group of 400 women, so we have developed algorithms to determine ovulatory status, day of ovulation and menstrual cycle disturbances such as short luteal phase, which will also be applied to the new sample. This previously-collected sample will serve as a baseline comparison group, as well as a source of data on the effects of lifestyle factors (such as smoking and alcohol consumption) on hormone function. The availability of this previously assembled cohort allows for more efficient examination of a small group of exposed women. This will be one of the first studies to examine biomarkers of exposure in relation to patterns of the menstrual cycle assessed directly by daily hormone levels. In addition to serum pesticide levels, questionnaire measures of exposure (e.g. species-specific fish consumption) will be examined in relation to ovarian function defined as continuous measures (e.g. cycle length) and dichotomous "abnormalities" (e.g. short luteal phase or anovulation). If interesting associations are revealed, follow-up in larger populations, with a larger gradient of exposure, will be warranted.