SEQUENCE SPECIFICITY IN A PROTEIN/DNA COMPLEX

蛋白质/DNA 复合物的序列特异性

• 批准号: 2546131
• 负责人: MARTHA P BROWN
• 金额: $ 2.86万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2546131
• 关键词: DNA; DNA binding protein; acidity /alkalinity; intermolecular interaction; nucleic acid sequence; salts; stoichiometry; temperature; thermodynamics; water solution; xylose

The broad, long-term objective of this research proposal is to understand the origin of specificity in protein-DNA interactions. Specifically, the research outlined in this proposal involves thermodynamically characterizing E. coil tryptophan repressor protein-DNA interactions and examining the role of water in these binding events. Ultimately, the results of this research will contribute to the information base regarding transcriptional regulation, which will assist in the understanding and treatment of disease states involving transcriptional regulation.

这项研究计划的广泛和长期目标是了解 蛋白质-DNA相互作用中特异性的起源。具体而言是 这项提案中概述的研究涉及到化学上的 表征E.螺旋色氨酸阻遏蛋白-DNA相互作用， 研究水在这些结合事件中的作用。最终 这项研究的结果将有助于信息库 关于转录调控，这将有助于 理解和治疗涉及转录的疾病状态 调控