SEQUENCE SPECIFICITY IN A PROTEIN/DNA COMPLEX

这项研究提案的广泛、长期目标是理解 蛋白质-DNA相互作用中特异性的起源。具体地说， 这项提案中概述的研究涉及热力学 大肠杆菌色氨酸抑制蛋白-DNA相互作用的特征 研究水在这些结合事件中的作用。归根结底， 这项研究的成果将有助于信息库的建立 关于转录调控，这将有助于 对涉及转录的疾病状态的理解和治疗 监管。

The broad, long-term objective of this research proposal is to understand the origin of specificity in protein-DNA interactions. Specifically, the research outlined in this proposal involves thermodynamically characterizing E. coil tryptophan repressor protein-DNA interactions and examining the role of water in these binding events. Ultimately, the results of this research will contribute to the information base regarding transcriptional regulation, which will assist in the understanding and treatment of disease states involving transcriptional regulation.