P2T-PURINERGIC RECEPTORS

P2T-嘌呤能受体

• 批准号: 2460149
• 负责人: JOSE L BOYER
• 金额: $ 9.76万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2460149
• 关键词: G protein; Xenopus oocyte; adenosine diphosphate; adenosine triphosphate; adenylate cyclase; biological signal transduction; complementary DNA; enzyme activity; enzyme inhibitors; human tissue; inhibitor /antagonist; isozymes; molecular cloning; phospholipase C; platelets; purinergic receptor; receptor coupling; receptor expression; second messengers; stimulant /agonist; tissue /cell culture

DESCRIPTION (Adapted from investigator's abstract): Platelet P2T- purinergic receptors are unique among P2-purinergic receptors since ADP is the preferred agonist and ATP an antagonist. Preliminary studies indicate that at least two cell lines that express P2T-purinergic receptors have been identified. The specific aims are: 1) To identify a model cell line for the study of the P2T-purinergic receptors. 2) To determine the signalling mechanism(s) and the G-protein(s) and phospholipase C involved in the activation of the P2T-purinergic receptors. 3) To clone the cDNA encoding the receptor from a human megakaryocytic cell line. 4) To stably express the cloned P2T- purinergic receptor(s) to establish its signalling mechanisms and to provide a model for eventual development of more potent and specific agonists and antagonists.

描述（根据研究者的摘要改编）：Platlet P2T- 自ADP以来 是首选的激动剂和ATP。初步研究 表明至少两种表达P2T-吡啶能的细胞系 已经确定了受体。具体目的是：1）确定 用于研究P2T-吡啶能受体的模型细胞系。 2）到 确定信号机制和G蛋白和G蛋白 磷脂酶C参与P2T-Purinagic的激活 受体。 3）克隆从人类中编码受体的cDNA 巨细胞细胞系。 4）稳定表达克隆的P2T- 嘌呤能受体（S）建立其信号传导机制和至 为最终开发更有效和具体的开发提供了模型 激动剂和对手。